FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1206364
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCCTCAAATCTCAGGCTGTTCAGTTCCATGTAGG44840.37169544184010794No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36210.30015816121833877No Hit
GTCTATTACTGTGCGATAGGTTGGGGTGGCAACTGGGCCGGGGACTATTGGGGCCAGGGAACTCTGGTCACCGT31450.2607007503539562No Hit
GTATGGTAGTACACCTCAGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT30620.25382057156878024No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA30500.2528258469251404No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29740.24652592418208769No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGACACTTTTAGCAC26830.2224038515738202No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA26550.22008282740532706No Hit
CTACCATACTGCTGACAGTAATAAACAGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC25890.21461184186530763No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT23110.19156738762098338No Hit
GTCTTAGTAGTACCTACTTAGCCTGGTACCACCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCAT21260.1762320493648683No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20880.173082087993342No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT20680.17142421358727547No Hit
GTGTACTACCATACTGCTGACAGTAATAAACAGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGT20570.17051238266393892No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20560.17042948894363558No Hit
GTAGTACACCTCAGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA20550.17034659522333226No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20520.17009791406242228No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC20190.16736242129241258No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20150.16703084641119928No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19650.16288616039603304No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC19390.1607309236681466No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC19280.15981909274481004No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG18820.15600598161085708No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAGCTACAGGTGTCCAGTCCCAGGTGCA18700.15501125696721718No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTTCT18690.15492836324691386No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTCCTGGATGCACCATAGATGAGGAGCCTG18670.1547625758063072No Hit
CATCTATGGTGCATCCAGGAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA17980.14904290910537782No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG17640.14622452261506477No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC17390.14415217960748167No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17120.1419140491592919No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTTCTGCAGGGCCAGTCAGAGTCTTAGTAGTA16900.14009038731261875No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGGTGGTACCAGGCTAAGTAGGTACTACTAAGACTCT16790.13917855638928217No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA16720.1385983003471589No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC16660.13810093802533893No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTTCTGCAGGGCCAGTCAGAGTCTTAGTAGTACCTACTTAGCCTG16220.13445361433199265No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG16170.13403914573047604No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16160.13395625201017272No Hit
GTTCAGTTCCATGTAGGCTGTGCTCGTGTATTCGTCCGCGGTAATCGTGAGTCTGCCCTGGAACTTCTGTGCGTA15740.13047471575743308No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15650.12972867227470317No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15590.1292313099528832No Hit
GTACTACCATACTGCTGACAGTAATAAACAGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGT15500.1284852664701533No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC15450.12807079786863665No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCTGAGGTGTACTACCATACT15080.12500373021741365No Hit
CTTTTAGCACCTCTGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGACTTGAGTGGATGGGAGGGGTCATCC14770.12243402488801057No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA14680.12168798140528067No Hit
GTTTATTACTGTCAGCAGTATGGTAGTACACCTCAGCTCACTTTCGGCGG14510.12027878816012413No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT14420.11953274467739422No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13900.11522227122162133No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTCCTGGATGCACCATA13880.11505648378101467No Hit
GAACTGAACAGCCTGAGATTTGAGGACACGGCCGTCTATTACTGTGCGATAGGTTGGGGTGGCAACTGGGCCGGG13800.11439333401858809No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCA13610.11281835333282492No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13460.11157494752827506No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG13350.11066311660493848No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAGCCGGCAAGTCCGTGACATGCCACGT13300.11024864800342185No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCT13090.10850787987705204No Hit
CTTCTGCAGGGCCAGTCAGAGTCTTAGTAGTACCTACTTAGCCTGGTACCACCAGAAACCTGGCCAGGCTCCCAG13060.10825919871614206No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC13050.10817630499583873No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA13010.10784473011462543No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCCGGCCCGAGGTCCACCTGCTGCCGCC12980.10759604895371547No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGGT12980.10759604895371547No Hit
GTAATAGACGGCCGTGTCCTCAAATCTCAGGCTGTTCAGTTCCATGTAGGCTGTGCTCGTGTATTCGTCCGCGGT12870.10668421803037889No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCTGTTTATTACTGTCAGCAGTATGGTAGTACA12820.10626974942886226No Hit
GCCTACATGGAACTGAACAGCCTGAGATTTGAGGACACGGCCGTCTATTACTGTGCGATAGGTTGGGGTGGCAA12760.10577238710704233No Hit
GTAATAAACAGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA12680.10510923734461572No Hit
CCCCTGGACAAGGACTTGAGTGGATGGGAGGGGTCATCCCTATCTTTGGT12500.10361715037915588No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12160.10079876388884285No Hit
ATCCAGGAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCA12140.10063297644823618No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12120.10046718900762953No Hit
GTCCCAGGTGCAGCTGGGGCAGTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAG12070.1000527204061129No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)