FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1350611
Sequences flagged as poor quality0
Sequence length18-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTC39180.29009092921648055No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38830.2874995094812644No Hit
GGAATAATCAGCCTCATCCTCAGACTGGAGGCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACCTGGAGCCAGA38750.2869071849703579No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36970.2737279646026872No Hit
GATTATTCCTGTGCAGCATGGGATGACAGCCTGAATGGTCCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTA32000.2369298043626181No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT30480.22567563865539372No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT29970.22189956989836454No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGACCATTCAGGCTGTCATCCCAT28370.21005307968023362No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC28140.20835014671137728No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG27570.20412983457116815No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27250.20176053652754197No Hit
GAAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGCATGTTCTTGGAATTGTCTCTGGA25890.19169101984213072No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCA25390.18798899164896482No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT24160.17888200229377668No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC23740.1757722986115173No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT23530.1742174467703876No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA23320.17266259492925795No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTGACTACTATGCCATGACCTGGGTCCGCCAGGCTCCAGGGAAGGG22750.1684422827890488No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC22680.16792399884200557No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC22530.1668133903840558No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCA22230.16459217346815627No Hit
GTATAAACTGGTACCAGCAGGTCCCAGGAACGGCCCCCAAACTCCTCATCTATACTAATAATCAGCGGCCCTCAG22160.16407388952111304No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC22060.16333348388247987No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG22040.16318540275475324No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA21960.16259307824384667No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTA21900.16214883486066675No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG21540.1594833745615873No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG21180.15681791426250785No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20620.15267164268616204No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG20560.15222739930298212No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGACCATTCAGG19820.1467483975770966No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGG19790.14652627588550665No Hit
GTCTGAGGATGAGGCTGATTATTCCTGTGCAGCATGGGATGACAGCCTGAATGGTCCGGTGTTCGGCGGAGGGAC19670.14563778911914682No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC19330.143120409947794No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT19070.14119535528734772No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA19030.14089919303189447No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18830.1394183817546281No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGG18580.13756736765804514No Hit
TCCTCAGACTGGAGGCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACCTGGAGCCAGAGAATCGGTCAGGGACC18220.13490190735896568No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA17910.13260664987920281No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17870.13231048762374953No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT17700.13105179803807315No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17670.13082967634648318No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG17670.13082967634648318No Hit
GTATATTTCTGTGCGAAAGGGTCTTACGATTTTTGGAGTGGTTATCCCTTTGACTACTGGGGCCAGGGAACCCGG17630.13053351409102992No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAGGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGCCTCCAGT17550.12994118958012335No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC17310.12816421604740372No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCT17300.1280901754835404No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA17280.12794209435581377No Hit
CATCTATACTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAGGTCTGGCACCTCAGCCTC16920.1252766340567343No Hit
CCCCTGAGGGCCGCTGATTATTAGTATAGATGAGGAGTTTGGGGGCCGTT16880.12498047180128104No Hit
GCATGGGATGACAGCCTGAATGGTCCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAG16620.12305541714083479No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTG16590.12283329544924482No Hit
GAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACA16530.12238905206606492No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16240.1202418757140287No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCT15920.11787257767040252No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT15750.1166138880847261No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG15630.11572540131836628No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT15610.11557732019063964No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGACCATTCA15570.11528115793518637No Hit
CTCCTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATAGTATAAACTGGTACCAGCAGGTCCCAGGAACGGCCCC15510.11483691455200645No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCCGGGTTCCCTGGCCCCAGTAGTCAAAGGGATAACCA15480.11461479286041651No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15250.11291185989156019No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCCGGCCCGAGGTCCACCTGCTGCCGCC14920.11046852128407068No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACG14870.1100983184647541No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAG14840.10987619677316415No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGATGCCCAAGTCAGGTACTTCTCGC14780.10943195338998424No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC14770.10935791282612092No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGGTGGTGGAGT14680.10869154775135106No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGAT14600.1080992232404445No Hit
GGGATAACCACTCCAAAAATCGTAAGACCCTTTCGCACAGAAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTT14590.1080251826765812No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGT14570.10787710154885456No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAG14550.10772902042112792No Hit
GTATGTGCTACGACCACTACCACTAATAGCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGGT14530.10758093929340129No Hit
CTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAGG14390.10654437139931483No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAG14310.10595204688840829No Hit
GGCCATCAGTGGCCTCCAGTCTGAGGATGAGGCTGATTATTCCTGTGCAGCATGGGATGACAGCCTGAATGGTCC14300.10587800632454497No Hit
GTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGACCTGCTGGTACCAGTTTATACTATTACTTCCGATGTTGGA14280.10572992519681833No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAGGCTCAGCGGGAAGACCTTGGG14230.10535972237750174No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14220.10528568181363841No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC14210.10521164124977511No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC14200.10513760068591178No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC14040.1039529516640987No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGGT13910.10299042433387555No Hit
GGGCTGGAGTGGGTCTCAGCTATTAGTGGTAGTGGTCGTAGCACATACTA13820.10232405925910569No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13660.1011394102372926No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)