FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394311
Sequences flagged as poor quality0
Sequence length19-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG15130.3837072767434842No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA14130.3583465842951376No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAG13350.3385652441854272No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAG13260.33628278186507604No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC12630.3203055456226177No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG12260.31092208941672944No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAG10720.2718666230462757No Hit
GACCTAGGACGGTGACCTTGGTCCCAGCTCCGAAGACATAAGAATCACGA10630.26958416072592445No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC10600.26882333995247404No Hit
GGATTACCTGTGGGGGAAACAACATTGGAAGTAAAAGTGTGCACTGGTACCATCAGAAGCCAGGCCAGGCCC10250.25994709759555273No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC9790.2482811790693133No Hit
GCACAGGGGCCTGGCCTGGCTTCTGATGGTACCAGTGCACACTTTTACTTCCAATGTTGTTTCCCCCACAGGTAA9200.23331837052478882No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC8810.22342770046993363No Hit
CCCCAGGACAGACGGCCAGGATTACCTGTGGGGGAAACAACATTGGAAGT8420.21353703041507846No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGCTCCGAAGACATAAGAATCACGACTACTATCCCACAC8320.21100096117024378No Hit
CCCCTGTGCTGGTCGTCTATGATGATAGTGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTC8290.2102401403967934No Hit
GTATATTACTGTGCGAGACCCTTATGGTTCGGGGAGTTACTAGGGGGCCC8010.20313914651125636No Hit
ACCTAGGACGGTGACCTTGGTCCCAGCTCCGAAGACATAAGAATCACGAC7910.20060307726642168No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT7650.19400929722985155No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC7590.19248765568295076No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC7560.19172683490950038No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT7520.19071240721156651No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC7380.18716191026879797No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGACCTCCTATGTGCTGAC7140.1810753440811948No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAACTGGCTTTTT6920.17549599174255853No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG6750.1711846740263396No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTG6680.16940942555495533No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA6540.16585892861218682No Hit
CTGCACAGACTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGT6420.16281564551838523No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCACTATCATCATAGA6410.16256203859390175No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG6410.16256203859390175No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG6330.16053318319803403No Hit
ATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGC6300.15977236242458365No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGC6270.15901154165113324No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG6260.15875793472664976No Hit
GGATAGTAGTCGTGATTCTTATGTCTTCGGAGCTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCC6170.15647547240629858No Hit
CTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGGCCGTG6150.15596825855733165No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG6100.1547002239349143No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT6080.15419301008594738No Hit
CTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTAC6000.15216415469007966No Hit
GGCCAGGCCCCTGTGCTGGTCGTCTATGATGATAGTGACCGGCCCTCAGG5880.14912087159627804No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGT5830.14785283697386073No Hit
CTATTACTGTCAGGTGTGGGATAGTAGTCGTGATTCTTATGTCTTCGGAGCTGGGACCAAGGTCACCGTCCTAGG5830.14785283697386073No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG5790.14683840927592687No Hit
CTATCATCATAGACGACCAGCACAGGGGCCTGGCCTGGCTTCTGATGGTACCAGTGCACACTTTTACTTCCAATG5770.14633119542695994No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTAT5740.14557037465350955No Hit
CTTTTACTTCCAATGTTGTTTCCCCCACAGGTAATCCTGGCCGTCTGTCCTGGGGCCACTGACACCGAGGG5560.14100545001280715No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG5560.14100545001280715No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT5480.13897659461693943No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACT5420.13745495307003863No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAAGTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG5340.13542609767417088No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG5140.13035395918450157No Hit
GCACAGACTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGA5130.1301003522600181No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC5120.12984674533553464No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTATTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA5100.1293395314865677No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGA5010.1270570691662165No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT4970.12604264146828265No Hit
CCTCTCTCACTGCACAGACTCTGTGACCTCCTATGTGCTGACTCAGCCAC4910.12452099992138187No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA4860.12325296529896453No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGT4850.12299935837448105No Hit
TACTTATTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTT4830.12249214452551413No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTG4810.1219849306765472No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA4500.11412311601755976No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG4480.11361590216859281No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC4450.11285508139514241No Hit
GTGCTGGTCGTCTATGATGATAGTGACCGGCCCTCAGGGATCCCTGAGCG4440.11260147447065894No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC4440.11260147447065894No Hit
TGATGATAGTGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGAC4430.11234786754617548No Hit
GACTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTAC4420.11209426062169202No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG4410.11184065369720855No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG4360.11057261907479123No Hit
CTCACTGCACAGACTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG4330.10981179830134082No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG4330.10981179830134082No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT4300.10905097752789042No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG4300.10905097752789042No Hit
CAGTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGCGTGTTCTTGGAATTGTCTC4270.10829015675444002No Hit
CCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTACCTGTGGGGGAAACAACATTGGAAGTAAAAGTG4230.10727572905650615No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG4230.10727572905650615No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC4200.10651490828305575No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA4200.10651490828305575No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTATTGTTGCTCTGTTTGGAGGGTT4060.10296441134028723No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT4030.10220359056683684No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA3990.10118916286890296No Hit
CCCTTATGGTTCGGGGAGTTACTAGGGGGCCCTGGGCAGGGATACTGGGGCCAGGGAACCCTGGTCACCGTCTC3980.10093555594441951No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGCGG100.0025168632204.9317870
TAGTCTA150.008463436136.621270
AGGGGGG1100.083.8357370
TACTCCG400.001533219476.8494270
TCTGGAG4050.073.37063670
ATCAATA500.003714186161.47953870
CGTATGC850.052.477541
TCGTATG850.052.44403540
CAATTTA200.007096313452.22138258
AGTTGGC200.00741399451.64627547
AGACTAT200.00747424551.5406744
TAGACTT200.007695713551.1614535
ACGGGTA200.007707351.14199416
TCTCGCG3700.049.8482770
ATCTCGT900.049.44220737
AGACCGA850.048.1397425
ACGAGAC850.048.13363622
CCGTCTT800.047.3424247
TCTCGTA950.046.87578238
ATGCCGT900.045.8139344
Produced by FastQC (version 0.11.5)