FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394311
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACG14590.370012502821377No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGCGTGTTCTTGGAATTGTCTCTGGA13580.34439820344854694No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCA12680.321573580245035No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT12540.3180230833022665No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC11880.3012850262863577No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC11200.284039755421482No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10690.27110580227282527No Hit
GGATTACCTGTGGGGGAAACAACATTGGAAGTAAAAGTGTGCACTGGTACCATCAGAAGCCAGGCCAGGCCC10400.26375120146280473No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGG9610.24371625442861095No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGT9330.23661526054307389No Hit
GACCTAGGACGGTGACCTTGGTCCCAGCTCCGAAGACATAAGAATCACGA8870.22494934201683442No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT7890.20009586341745478No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC7830.19857422187055393No Hit
CCCCTGTGCTGGTCGTCTATGATGATAGTGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTG7780.19730618724813662No Hit
GCACAGGGGCCTGGCCTGGCTTCTGATGGTACCAGTGCACACTTTTACTT7670.1945165110788185No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAG7490.1899515864381161No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTA7470.18944437258914917No Hit
ACCTAGGACGGTGACCTTGGTCCCAGCTCCGAAGACATAAGAATCACGACTACTATCCCACACCTGACAGTAATA7450.18893715874018224No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCACTATCATCATA7250.18386502025051293No Hit
CCCCAGGACAGACGGCCAGGATTACCTGTGGGGGAAACAACATTGGAAGT7220.18310419947706252No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGCTCCGAAGACATAAGAATCACGACTACTATCCCACAC7130.18082173715671132No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC7120.18056813023222787No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGT7050.1787928817608436No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC6930.175749598667042No Hit
GTATATTACTGTGCGAGACCCTTATGGTTCGGGGAGTTACTAGGGGGCCC6810.17270631557324043No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGACCTCCTATGTGCTGACT6770.17169188787530656No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAACTGGCTTTTT6730.17067746017737268No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT6640.16839499785702147No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGCTCCGAAGACATAAGAATCAC6410.16256203859390175No Hit
CTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTAC6200.15723629317974896No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT6190.1569826862552655No Hit
CTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGAC6090.15444661701043083No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGT6050.153432189312497No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCG6030.15292497546353007No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTG5990.15191054776559618No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG5830.14785283697386073No Hit
GGATAGTAGTCGTGATTCTTATGTCTTCGGAGCTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCC5810.1473456231248938No Hit
CTGCACAGACTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAG5780.1465848023514434No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTG5730.14531676772902608No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGG5700.1445559469555757No Hit
GGCCAGGCCCCTGTGCTGGTCGTCTATGATGATAGTGACCGGCCCTCAGG5600.142019877710741No Hit
CTATTACTGTCAGGTGTGGGATAGTAGTCGTGATTCTTATGTCTTCGGAG5570.1412590569372906No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAAGTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGG5570.1412590569372906No Hit
CCTCTCTCACTGCACAGACTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACA5560.14100545001280715No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAG5280.1339044561272701No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCT5280.1339044561272701No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGACCTCCTA5270.13365084920278664No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTATTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTT5270.13365084920278664No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCT5250.1331436353538197No Hit
ATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGC5230.13263642150485278No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCAGGTGTGGGATAGTAGTC5130.1301003522600181No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG5080.12883231763760078No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAG5060.12832510378863385No Hit
CTATCATCATAGACGACCAGCACAGGGGCCTGGCCTGGCTTCTGATGGTA4950.12553542761931571No Hit
GTGCTGGTCGTCTATGATGATAGTGACCGGCCCTCAGGGATCCCTGAGCG4880.12376017914793144No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG4820.12223853760103065No Hit
GCACAGACTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAG4740.12020968220516294No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC4720.119702468356196No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC4680.11868804065826213No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG4680.11868804065826213No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGGCCGTGTTCCCAGAGTTGGAGCCA4650.11792721988481174No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC4640.11767361296032826No Hit
CTTTTACTTCCAATGTTGTTTCCCCCACAGGTAATCCTGGCCGTCTGTCCTGGGGCCACTGACACCGAGGGTG4550.11539115063997707No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG4530.11488393679101015No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG4500.11412311601755976No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA4410.11184065369720855No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC4330.10981179830134082No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG4310.10930458445237388No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGACCTCCTATGTGCTGACTCAGC4220.1070221221320227No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC4140.10499326673615497No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA4140.10499326673615497No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGACCTC4090.10372523211373763No Hit
GTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCAGGATTACCTGTGGGGGAAACAACATT4080.10347162518925418No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAG3980.10093555594441951No Hit
TGATGATAGTGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGAC3980.10093555594441951No Hit
GCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCAAGTATGCCATGA3970.10068194901993605No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)