FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1419063
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACACCCGTGTCGGCGGCGGTCACAGAGATCATCTTCAGGGAGAATTGGTTCCTGGGCGCATCCATGGA85190.6003257078790722No Hit
GTCTATTACTGTGCGATTGGGTATTACGATTTTTGGAGTAGTTCTTCAAA59390.41851559796851867No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT32730.23064515106094655No Hit
GTCTCCGGTGGCTCCGTCAGCGCCAGTAATTCCTACCGGGGGTGGGTCCGCCAGGCCCCAGGGAAGGGGCTGGAG31620.22282308819270177No Hit
CATCTATAGTGCATCCAGGAGGGCCACTGACATCCCAGACAAGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA30970.21824260092751344No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30600.21563524663809852No Hit
GTATTACTGTCAGCACTATCATACCTCATCGGTCACTTTCGGCGGAGGTACCAGGGTGGAGATCAGACGAACTG29440.20746083859560852No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT29130.2052762985152879No Hit
GTATGATAGTGCTGACAGTAATACGCTGCAAAATCTTCAGGCTCCAGTCTGCTGATGCTGAGAGTGAAGTCTGTC29080.20492395334104266No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA27310.1924509341727605No Hit
CTATCATACCTCATCGGTCACTTTCGGCGGAGGTACCAGGGTGGAGATCAGACGAACTGTGGCTGCACCA27250.19202811996366617No Hit
CTATAGATGAGGAGCCTGGGAGCCTGGCCAGGTCTTCGTTGGTACCAGGCTAAGTCGTTGTTAGGAACACTCTGA25650.18075307438781787No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC25650.18075307438781787No Hit
CAGTAATACGCTGCAAAATCTTCAGGCTCCAGTCTGCTGATGCTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA24730.17426992318170512No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT24240.17081694047410156No Hit
CACCTGCACTGTCTCCGGTGGCTCCGTCAGCGCCAGTAATTCCTACCGGG23910.16849146232408285No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23550.165954577069517No Hit
ATCATACCTCATCGGTCACTTTCGGCGGAGGTACCAGGGTGGAGATCAGACGAACTGTGGCTGCACCATCTGTCT22780.16052846138614002No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC22470.1583439213058194No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC22140.1560184431558007No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA22060.15545469087700828No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG21900.15432718631942346No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21830.1538339030754801No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT21440.15108561071636706No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA21270.1498876371239332No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG21080.1485487254618012No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG20810.1466460615208768No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA20760.14629371634663155No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC20320.14319307881327326No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT19750.13917634382687732No Hit
AGGTATGATAGTGCTGACAGTAATACGCTGCAAAATCTTCAGGCTCCAGTCTGCTGATGCTGAGAGTGAAG19710.1388944676874811No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19530.13762602506019816No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTCCTAACAACGACTTAGCCTGGTACC19060.13431398042229273No Hit
GTCCCAGACCCACTGCCACTGAACTTGTCTGGGATGTCAGTGGCCCTCCTGGATGCACTATAGATGAGGAGCC18850.13283413069046265No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18740.13205897130712307No Hit
GTCTGGGACAGACTTCACTCTCAGCATCAGCAGACTGGAGCCTGAAGATTTTGCAGCGTATTACTGTCAGCACTA18370.1294516170177082No Hit
CTATTACTGTGCGATTGGGTATTACGATTTTTGGAGTAGTTCTTCAAATTACCCCTTCCCTTTGGACGTCTGGGG18290.12888786473891575No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18250.12860598859951955No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18220.1283945814949724No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG18170.12804223632072714No Hit
CAGTAATAGACACCCGTGTCGGCGGCGGTCACAGAGATCATCTTCAGGGAGAATTGGTTCCTGGGCGCATCCA18050.1271966079025385No Hit
GTCTTTGTCTCCTGGGGAAACAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTCCTAACAACGACTTAGCC17990.12677379369344421No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC17710.12480066071767074No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17690.12465972264797263No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17210.12127720897521815No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG17210.12127720897521815No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC16920.11923360696459566No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCTGGGGAAACAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTCCTAA16890.1190221998600485No Hit
ACCGGAGACAGTGCAGGTGAGGGACAGGGTCTCCGAAGGCTTCACCAGTCCTGGGCCCGACTGTTGCAGCTGCAA16820.11852891661610512No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT16790.11831750951155798No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC16750.11803563337216176No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16740.11796516433731272No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG16550.11662625267518073No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGGGCTTCTTCCCCCAGGAGCCACTCAG16430.1157806242569921No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16050.11310280093272815No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC15780.11120013699180374No Hit
GGGTTGGGAGTAACCATTTAGGTGGGCGCAACTACTACAACCCGTCCCTCAAAAGTCGAGTCTCCATATCCA15770.1111296679569547No Hit
CCTAAATGGTTACTCCCAACCCACTCCAGCCCCTTCCCTGGGGCCTGGCGGACCCACCCCCGGTAGGAATTAC15610.11000216339936987No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC15560.1096498182251246No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACTTGTCTGGGATGTCAGTGGCCCTCCTGGATGCACTATAGA15450.10887465884178503No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT15430.10873372077208694No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG15030.10591495937812487No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCG14820.10443510964629477No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT14560.10260291474021943No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14540.10246197667052133No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT14470.10196869342657797No Hit
GTCCCTCACCTGCACTGTCTCCGGTGGCTCCGTCAGCGCCAGTAATTCCT14470.10196869342657797No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT14470.10196869342657797No Hit
CTCCAGGCACCCTGTCTTTGTCTCCTGGGGAAACAGCCACCCTCTCCTGC14420.1016163482523327No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8400.043.7115251
TGTTAGA10250.038.71992570
CTTATAC2250.037.428831
CGTATGC2400.035.7012741
GACAGGT2850.035.4588931
TATACAC2600.034.9643023
CGTCTGG18600.034.34673369
CATCCAT21950.033.9649466
TTATACA2600.033.6693272
TCGTATG2550.033.5446440
GTATCAA18550.033.231961
ATCTCGT2400.032.629837
GCGTAGA19850.032.62165570
GGCGCAT22550.032.50008862
CGCATCC22850.032.1787364
GCGCATC22850.032.04388463
CCCGTGT22500.031.72175211
TCTATTA13800.031.7174842
ACCCGTG22400.031.71306810
CGTGTCG22550.031.65141713
Produced by FastQC (version 0.11.5)