FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1419063
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACACCCGTGTCGGCGGCGGTCACAGAGATCATCTTCAGGGAGAATTGGTTCCTGGGCGCATCCATG68500.4827128887160049No Hit
GTCTATTACTGTGCGATTGGGTATTACGATTTTTGGAGTAGTTCTTCAAATTACCCCTTCCCTTTGGACGTCTGG54230.3821535759864079No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31580.22254121205330563No Hit
GTCTCCGGTGGCTCCGTCAGCGCCAGTAATTCCTACCGGGGGTGGGTCCG31370.22106136232147552No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA29750.20964537867592914No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29090.2049944223758917No Hit
CATCTATAGTGCATCCAGGAGGGCCACTGACATCCCAGACAAGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA28530.20104815642434481No Hit
GTATGATAGTGCTGACAGTAATACGCTGCAAAATCTTCAGGCTCCAGTCTGCTGATGCTGAGAGTGAAGTCTGTC28170.19851127116977896No Hit
GTATTACTGTCAGCACTATCATACCTCATCGGTCACTTTCGGCGGAGGTACCAGGGTGGAGATCAGACGAACTGT28140.19829986406523178No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC27850.19625626205460925No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG27110.19104155347577945No Hit
CTATCATACCTCATCGGTCACTTTCGGCGGAGGTACCAGGGTGGAGATCAGACGAACTGTGGCTGCACCAT25180.17744102974991244No Hit
CACCTGCACTGTCTCCGGTGGCTCCGTCAGCGCCAGTAATTCCTACCGGGGGTGGGTCCGCCAGGCCCCAGGGAA24680.17391757800745986No Hit
CTATAGATGAGGAGCCTGGGAGCCTGGCCAGGTCTTCGTTGGTACCAGGCTAAGTCGTTGTTAGGAACACTCT24230.17074647143925253No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC23880.1682800552195357No Hit
CAGTAATACGCTGCAAAATCTTCAGGCTCCAGTCTGCTGATGCTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA23780.16757536487104518No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC22320.15728688578308364No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG22300.15714594771338553No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22100.15573656701640448No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA21830.1538339030754801No Hit
ATCATACCTCATCGGTCACTTTCGGCGGAGGTACCAGGGTGGAGATCAGA21660.15263592948304622No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT21500.15150842492546138No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA21010.14805544221785782No Hit
GGGTTGGGAGTAACCATTTAGGTGGGCGCAACTACTACAACCCGTCCCTCAAAAGTCGAGTCTCCATATCCATG20740.14615277827693343No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG20480.1443205833708581No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG20230.1425588574996318No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG20000.1409380696981036No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC19470.13720321085110387No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC19110.134666325596538No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA18250.12860598859951955No Hit
GTCTGGGACAGACTTCACTCTCAGCATCAGCAGACTGGAGCCTGAAGATT18080.12740801500708568No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCT17930.1263509794843499No Hit
GTCTTTGTCTCCTGGGGAAACAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTCCTAACAACGACTTAGCCTG17900.12613957237980275No Hit
AGGTATGATAGTGCTGACAGTAATACGCTGCAAAATCTTCAGGCTCCAGTCTGCTGATGCTGAGAGTGAAGT17860.12585769624040652No Hit
ACTACAACCCGTCCCTCAAAAGTCGAGTCTCCATATCCATGGATGCGCCCAGGAACCAATTCTCCCTGAAGAT17810.12550535106616129No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17710.12480066071767074No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAGCCGGCAAGTCCGTGACATGCCACGT17420.12275705870704824No Hit
GTCCCAGACCCACTGCCACTGAACTTGTCTGGGATGTCAGTGGCCCTCCTGGATGCACTATAGATGAGGAGCCTG17400.12261612063735013No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17350.12226377546310488No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTCCTAACAACGACTTAGCCTGGTACCAACGAAGACCTGGCCAGGCTCCCAG17140.1207839257312748No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17000.11979735924338805No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC16530.11648531460548264No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG16480.11613296943123737No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC16400.11556921715244496No Hit
CTCCATATCCATGGATGCGCCCAGGAACCAATTCTCCCTGAAGATGATCTCTGTGACCGCCGCCGACACGGGTGT16360.11528734101304876No Hit
CTATTACTGTGCGATTGGGTATTACGATTTTTGGAGTAGTTCTTCAAATTACCCCTTCCCTTTGGACGTCTGGG16290.1147940577691054No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC16190.11408936742061486No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16050.11310280093272815No Hit
GGGCTGGAGTGGGTTGGGAGTAACCATTTAGGTGGGCGCAACTACTACAA15970.11253904865393574No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15890.11197529637514332No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA15780.11120013699180374No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGGT15760.11105919892210564No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC15730.1108477918175585No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCTGGGGAAACAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTCCTAA15700.11063638471301135No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCGT15030.10591495937812487No Hit
CAGTAATAGACACCCGTGTCGGCGGCGGTCACAGAGATCATCTTCAGGGAGAATTGGTTCCTGGGCGCATCCATG15030.10591495937812487No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCCGGCCCGAGGTCCACCTGCTGCCGCC14940.1052807380644834No Hit
ACCGGAGACAGTGCAGGTGAGGGACAGGGTCTCCGAAGGCTTCACCAGTCCTGGGCCCGACTGTTGCAGCTGCAA14890.10492839289023813No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14820.10443510964629477No Hit
CCATTTAGGTGGGCGCAACTACTACAACCCGTCCCTCAAAAGTCGAGTCTCCATATCCATGGATGCGCCCAGGAA14790.10422370254174762No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT14790.10422370254174762No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACTTGTCTGGGATGTCAGTGGCCCTCCTGGATGCACTATA14690.10351901219325711No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14530.10239150763567226No Hit
CCTAAATGGTTACTCCCAACCCACTCCAGCCCCTTCCCTGGGGCCTGGCGGACCCACCCCCGGTAGGAATTACTG14480.10203916246142701No Hit
GTCCCTCACCTGCACTGTCTCCGGTGGCTCCGTCAGCGCCAGTAATTCCT14330.10098212693869124No Hit
CTCCAGGCACCCTGTCTTTGTCTCCTGGGGAAACAGCCACCCTCTCCTGC14320.10091165790384218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATTA1051.6892955E-890.7448570
GAGGGTT1551.8053694E-551.2269370
AACTGTA950.00832681450.1484770
CTGGAGT21950.045.57913270
AATGGTA3703.6379788E-1142.9198670
CGTCTGG19050.041.16644769
CTTAGCA1650.001513940538.49781870
CTGTCTT40350.037.3886870
GAGCCAT18750.036.4189370
GGTATCA8450.035.8200871
TAACCGT450.005270777735.2896670
GAGCAAA20600.034.6900870
AGCAGAA2751.3005516E-534.64803370
AAGGACA20950.034.11053570
GACAGGT3750.034.07951
GTATCAA19250.034.067851
AGAACCA12600.034.02931670
AGATCTT2402.3767448E-433.0840670
ACGGTAA450.004315302231.10143553
CAAACGG450.00445027530.90629250
Produced by FastQC (version 0.11.5)