FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences965534
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGAAGCTCACCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGT41530.4301246771216757No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35280.36539365781008226No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA31080.32189441283269155No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATCGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA27390.2836772190311268No Hit
GTAATAAAGAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTGAGTTCTTGGCGTTGTCTCTGGA26830.27787731970080803No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25440.2634811410059097No Hit
GTGTTAGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCAT23090.23914227774475058No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT22970.23789944217396797No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA21850.22629964351333046No Hit
CTTTATTACTGTGCGAGAGATGTCCTTGATTTTTGGAGTACTTATGAGACTCCTGAATGGGCCTCGGTCCCCTA21590.22360683310996815No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC21550.22319255458637396No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20560.2129391611274176No Hit
GTGTATTACTGTCAGCAGTATGGAAGCTCACCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA20490.21221417371112772No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC20020.2073464010588959No Hit
GTATGGAAGCTCACCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT18470.19129310826962073No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17800.18435394299941793No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA17540.18166113259605565No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC17330.1794861703471861No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16950.17555052437304125No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATCGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA16730.17327199249327316No Hit
GTCTGGGACAGACTTCACTCTCACGATCAGCAGACTGGAGCCTGAAGATT16600.17192558729159202No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCTCCCTCTCCTGCAGGGCCAGTCACAGTGTTAGCAGCAGCTACTTAGCCTG16570.17161487839889636No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA16060.16633282722307033No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA15650.16208647235622983No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTGCTGCTAACACTGT15370.15918652269107042No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15230.15773654785849076No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGATGCACCATAGATGAGGAGCCTG15210.15752940859669362No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14830.15359376262254879No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT14330.1484152810776213No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCTCCCTCTCCTGCAGGGCCAGTCACAGTGTTAGCA14030.14530819215066482No Hit
GTATTTAGTACTACCACTACTACTAATGTATGAAACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTT13900.14396178694898368No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCTCCCTCTCCTGCAGGGCCAGTCACAGTGTTAGCAGCA13260.1373333305714765No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13260.1373333305714765No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13260.1373333305714765No Hit
ACTAAATACTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCACTGTATCT13240.1371261913096794No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC13160.136297634262491No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA12930.13391553275182438No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG12810.13267269718104177No Hit
CTTCCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATCGTGAGAGTGAAGTCTGTC12750.1320512793956505No Hit
GTCACAGTGTTAGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATG12600.13049773493217226No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12530.12977274751588241No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12520.12966917788498386No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC12360.12801206379060706No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11890.12314429113837524No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCA11800.1222121644602883No Hit
CTCCTGCAGGGCCAGTCACAGTGTTAGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAG11630.12045148073501295No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA11590.12003720221141875No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG11540.11951935405692601No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGTTATGAAATGAACTGGGTCCGCCAGGCTCCAGGGAAGGG11280.11682654365356372No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG11280.11682654365356372No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT11170.11568727771367968No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11100.11496229029738984No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC10620.10999094801425946No Hit
GTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTT10510.10885168207437543No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT10440.10812669465808558No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC10400.10771241613449138No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10240.1060553020401146No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC10240.1060553020401146No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10230.10595173240921604No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG10140.10501960573112909No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10140.10501960573112909No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10000.10356963089854941No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9940.1029482131131581No Hit
GTAGGGGACCGAGGCCCATTCAGGAGTCTCATAAGTACTCCAAAAATCAAGGACATCTCTCGCACAGTAATAAA9830.10180894717327406No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9820.10170537754237552No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCA9680.10025540270979583No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT200.005260516160.1952270
TACTCAT650.001788755473.9362670
GAGCCAT10100.065.0297470
AATGGTA3000.064.0780970
GTACAAA1152.4461802E-455.72007870
AACAATT900.006493211353.39840770
GAGCAAA18550.044.9064870
CACTCGT1454.6635796E-744.19178870
ATGGCAA1650.001449306638.83520570
CCGTTAA350.001476534438.70297616
GCCGTTA350.001477286638.69896715
GGTATCA7550.037.6874921
GTATCAA15800.037.0897521
CACCGAT400.002814869633.93543627
AGCCATA400.002849429433.851072
TGAGCTT13300.033.7253170
GAACTGT17950.033.301869
ATGCTAG751.4127108E-632.89422262
ATCAACG18750.031.5926973
TCTTGTT3602.1511205E-631.14907370
Produced by FastQC (version 0.11.5)