FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1305045
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCGTCAGATTTGAGGCTCCTCAATTCCATGTAGGCTGTGCTCGTGGATGTGTCTGTGG54630.4186062549567256No Hit
GTAGTAGTCAGCCTCGTCCTCAGGCTGGAGTCCAGTTATGGTCAGGGAGG41190.3156213004149282No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC33010.252941469451245No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT27840.21332597726515176No Hit
GACTACTACTGCGCAGCATGGGACAGCAGCCTCAGTGCTCAGGTCTTCGGAACTGGGACCAGGGTCACCGTCC27280.20903493749257687No Hit
GCATGGGACAGCAGCCTCAGTGCTCAGGTCTTCGGAACTGGGACCAGGGT27220.2085751832312296No Hit
GTCCCATGCTGCGCAGTAGTAGTCAGCCTCGTCCTCAGGCTGGAGTCCAGTTATGGTCAGGGAGGCTGTGTTAC26520.20321138351551093No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTTCTCGATGGGGGCTGGGAGGCCTTTG26450.20267500354393908No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGGACGGTGACCCTGGTCCCAG26100.19999310368607978No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAG25800.19769433237934322No Hit
CAGTAATACACGGCCGTGTCGTCAGATTTGAGGCTCCTCAATTCCATGTAGGCTGTGCTCGTGGATGTGTCTG24140.18497446448206767No Hit
GTGTATTACTGTGCGAGAGATCCGGTCGTCTTTTCGTACAGTAACTGGGGGGGTGATGCTTTTGATATCTGGGGC23930.18336532456735208No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAG23730.18183281036286106No Hit
CACCTGCACTGGGAACAGCAATAATGTTGGCTACCAAGGAGCTGCTTGGCTGCAGCAATACCAGGGCCTCCCTCC23690.18152630752196283No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC23580.18068342470949278No Hit
GTCCTATCCTACAGGAATAACTTCCGGCCCTCTGGGATCTCAGAGAGATT23370.1790742847947772No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG23090.17692876490848974No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCT22800.17470661931197776No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC21360.16367251703964233No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGAAGCGGTTGTGCAG21330.16344263990896868No Hit
CAGTAAAGGTCTCCTGCAAGACTTCTGGTTACACATTCACCAGTTATGGTCTCAGCTGGGTGCGACAGGCCCC21200.16244650567604949No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC21190.16236987996582494No Hit
GGCTTGGGACAGACCGCCACACTCACCTGCACTGGGAACAGCAATAATGTTGGCTACCAAGGAGCTGCTTGGC20550.15746583451145363No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGACAG19920.15263841476730686No Hit
GTGTTACCTGACCTGGATGCAGAGAATCTCTCTGAGATCCCAGAGGGCCGGAAGTTATTCCTGTAGGATAGGAC19780.15156565482416315No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA19440.1489603806765284No Hit
GTGTTGTAGTTGTTCTCCGGCTGCCCGCTGCTCTCCCACTCCACGGCGAT19240.14742786647203737No Hit
GTATTACTGTGCGAGAGATCCGGTCGTCTTTTCGTACAGTAACTGGGGGG19140.14666160936979183No Hit
GTCTGGAGGTGAAGTGAAGAAGCCTGGGGCCTCAGTAAAGGTCTCCTGCAAGACTTCTGGTTACACATTCACCAG18680.14313682669946245No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCCTGGAGCAGGGCGCCAGGGGGAAGACCGATGGGCCCTTGG18660.14298357527901337No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC18450.14137443536429778No Hit
GCCTTGGGCTGACCCAGGACGGTGACCCTGGTCCCAGTTCCGAAGACCTGAGCACTGAGGCTGCTGTCCCA18400.14099130681317504No Hit
GTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC17270.1323326015578007No Hit
GTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA17260.13225597584757615No Hit
GTTATGGTCTCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTCAGTGG16690.1278883103647767No Hit
GCAATAATGTTGGCTACCAAGGAGCTGCTTGGCTGCAGCAATACCAGGGC16610.1272753046829803No Hit
CCCCTGGACAAGGGCTTCAGTGGATGGGGTGGATCAGCGCTTACAACGGT16440.1259726676091629No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAAGTGGTACGT15650.11991923650142332No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGGACGGTGACCCTGGTC15580.11938285652985146No Hit
GGACAATGGTCACCATCTCCTCAGCTTCCACCAAGGGCCCATCGGTCTTCCCCCTGGCGCCCTGCTCCAGGAGCA15290.11716071093333946No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15210.11654770525154304No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA15090.11562819672884843No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT15080.11555157101862387No Hit
CCTTGGGAGAATCCCCTAGATCACAGCTCCTCACCATGGACTGGACCTGGAGCATCCTTTTCTTGGTGGCAGCAG14850.11378917968345918No Hit
GTCCAAGGGCTTGGGACAGACCGCCACACTCACCTGCACTGGGAACAGCAATAATGTTGGCTACCAAGGAGC14810.11348267684256098No Hit
CCCTTATGATTTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGT14710.11271641974031547No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC14680.11248654260964182No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG14370.1101111455926807No Hit
GTATCAACGCAGAGTACGGGACCCAAAAACCACACCCCTCCTTGGGAGAATCCCCTAGATCACAGCTCCTCACCA14230.109038385649537No Hit
GGATAGGACCTTGGGAGGGAGGCCCTGGTATTGCTGCAGCCAAGCAGCTCCTTGGTAGCCAACATTATTGCTGT14010.10735262002459685No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC13940.106816240053025No Hit
GTCCTGCACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTC13870.10627986008145314No Hit
CCTCAATTCCATGTAGGCTGTGCTCGTGGATGTGTCTGTGGTCATGGTGA13810.10582010582010583No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG13670.10474734587696209No Hit
GGGTGGATCAGCGCTTACAACGGTTACACAAACTATGCACAGAAATTTCA13570.10398108877471658No Hit
CCCAAGGTCCTATCCTACAGGAATAACTTCCGGCCCTCTGGGATCTCAGA13560.10390446306449203No Hit
CCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAAC13520.10359796022359383No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG13510.10352133451336928No Hit
GTCCAGGGGCCTGTCGCACCCAGCTGAGACCATAACTGGTGAATGTGTAACCAGAAGTCTTGCAGGAGACCTTTA13500.10344470880314471No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTTCAACAGCACGTTCCGTG13460.10313820596224652No Hit
GTAACTGGGGGGGTGATGCTTTTGATATCTGGGGCCAAGGGACAATGGTC13260.10160569175775547No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTC13240.10145244033730638No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)