FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1305045
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCGTCAGATTTGAGGCTCCTCAATTCCATGTAGGCTGTGCTCGTGGATGTGTCTGTGGT47530.36420200069729397No Hit
GTAGTAGTCAGCCTCGTCCTCAGGCTGGAGTCCAGTTATGGTCAGGGAGGCTGTGTTACCTGACCTGGATGCAGA34910.2675003543939098No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29500.22604584516242734No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTC27330.20941806604369964No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT27230.20865180894145413No Hit
GTCCCATGCTGCGCAGTAGTAGTCAGCCTCGTCCTCAGGCTGGAGTCCAGTTATGGTCAGGGAGGCTGTGTTAC25770.1974644552486696No Hit
GACTACTACTGCGCAGCATGGGACAGCAGCCTCAGTGCTCAGGTCTTCGGAACTGGGACCAGGGTCACCGTCCTG25590.19608519246462766No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC25020.19171752698182823No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA24850.19041488990801084No Hit
GTGTATTACTGTGCGAGAGATCCGGTCGTCTTTTCGTACAGTAACTGGGGGGGTGATGCTTTTGATATCTGGGGC24300.1862004758456605No Hit
GTCCTATCCTACAGGAATAACTTCCGGCCCTCTGGGATCTCAGAGAGATTCTCTGCATCCAGGTCAGGTAACACA24190.18535759303319044No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT24060.18436145880027127No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGG23620.18098992755039098No Hit
GCATGGGACAGCAGCCTCAGTGCTCAGGTCTTCGGAACTGGGACCAGGGT23370.1790742847947772No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT23220.17792489914140894No Hit
CACCTGCACTGGGAACAGCAATAATGTTGGCTACCAAGGAGCTGCTTGGCTGCAGCAATACCAGGGCCTCCCTCC22920.17562612783467238No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGT22770.1744767421813041No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGG21450.16436214843166327No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC21190.16236987996582494No Hit
GTGTTGTAGTTGTTCTCCGGCTGCCCGCTGCTCTCCCACTCCACGGCGAT20610.15792558877280094No Hit
CAGTAATACACGGCCGTGTCGTCAGATTTGAGGCTCCTCAATTCCATGTAGGCTGTGCTCGTGGATGTGTCTGTG20490.15700608025010632No Hit
GGCTTGGGACAGACCGCCACACTCACCTGCACTGGGAACAGCAATAATGTTGGCTACCAAGGAGCTGCTTGGCT20200.15478393465359433No Hit
GTGTTACCTGACCTGGATGCAGAGAATCTCTCTGAGATCCCAGAGGGCCGGAAGTTATTCCTGTAGGATAGGAC19630.15041626917079487No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAACCAAAGGACA19570.14995651490944756No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC19570.14995651490944756No Hit
CAGTAAAGGTCTCCTGCAAGACTTCTGGTTACACATTCACCAGTTATGGTCTCAGCTGGGTGCGACAGGCCCCTG19380.14850062641518108No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGAAGCGGTTGTGCAGAGCCTCATGCATCACGGAGCATGA18540.14206406675631875No Hit
GTCTGGAGGTGAAGTGAAGAAGCCTGGGGCCTCAGTAAAGGTCTCCTGCA18380.14083805539272592No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA18320.1403783011313786No Hit
GTATTACTGTGCGAGAGATCCGGTCGTCTTTTCGTACAGTAACTGGGGGG18280.14007179829048041No Hit
GCCTTGGGCTGACCCAGGACGGTGACCCTGGTCCCAGTTCCGAAGACCTGAGCACTGAGGCTGCTGTCCCAT17570.13463137286453725No Hit
GTCTTCCCCCTGGCGCCCTGCTCCAGGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA16540.12673892471140843No Hit
GGGTGGATCAGCGCTTACAACGGTTACACAAACTATGCACAGAAATTTCA16080.12321414204107903No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15870.12160500212636345No Hit
GCAATAATGTTGGCTACCAAGGAGCTGCTTGGCTGCAGCAATACCAGGGCCTCCCTCCCAAGGTCCTATCCTACA15720.12045561647299517No Hit
GTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAG15700.12030236505254609No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCCTGGAGCAGGGCGCCAGGG15420.11815684516625863No Hit
CCCCTGGACAAGGGCTTCAGTGGATGGGGTGGATCAGCGCTTACAACGGT15290.11716071093333946No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGC15190.11639445383109395No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTA15100.11570482243907299No Hit
GTATCAACGCAGAGTACGGGACCCAAAAACCACACCCCTCCTTGGGAGAA14910.1142489339448065No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC14900.11417230823458195No Hit
GTTATGGTCTCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTCAGTGGATGGGGTGGATCAGCGCTTACAACG14760.11309954829143821No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGAACTGCTCCTCCCGCGGCTTTGTCTT14750.11302292258121367No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAAGTGGTACGT14640.1121800397687436No Hit
GGACAATGGTCACCATCTCCTCAGCTTCCACCAAGGGCCCATCGGTCTTCCCCCTGGCGCCCTGCTCCAGGAGCA14630.11210341405851905No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGGACGGTGACCCTGGTCCCAGTTCCGAAGACCTGAGCACTGA14600.1118735369278454No Hit
CCCTTATGATTTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG14560.1115670340869472No Hit
CCTTGGGAGAATCCCCTAGATCACAGCTCCTCACCATGGACTGGACCTGGAGCATCCTTTTCTTGGTGGCAGCA14140.10834875425751603No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14120.10819550283706691No Hit
CCTCAATTCCATGTAGGCTGTGCTCGTGGATGTGTCTGTGGTCATGGTGACTCTGTCCTGAAATTTCTGTGCATA14080.10788899999616872No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTT14060.10773574857571962No Hit
GTCCAAGGGCTTGGGACAGACCGCCACACTCACCTGCACTGGGAACAGCAATAATGTTGGCTACCAAGGAGCT14020.1074292457348214No Hit
GTCCAGGGGCCTGTCGCACCCAGCTGAGACCATAACTGGTGAATGTGTAACCAGAAGTCTTGCAGGAGACCTTTA13810.10582010582010583No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC13720.10513047442808486No Hit
GGATAGGACCTTGGGAGGGAGGCCCTGGTATTGCTGCAGCCAAGCAGCTCCTTGGTAGCCAACATTATTGCTGTT13190.10106931178618361No Hit
GGGGAGGTGTGTCACAAGATTTGGGCTCTGGGCACCGTGGGCATGTGTGAGTTGTGTCACCAAGTGGGGTTTTGA13060.10007317755326445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCGT1053.3087417E-882.3668270
GTCGTTT951.7584262E-675.8641870
GGGCGTT650.002720373266.52705470
CCTTTGT13800.060.58139470
AACGGCG200.00701158352.38718453
TCGGTAG200.0079720950.71343628
TACGGTC305.260018E-447.7327763
GGGCCAA12900.046.9299470
GACAGGT3650.039.777791
GGCATTA9900.039.3114470
CCCGTCA15200.037.9320970
CAGGAAA1901.06374486E-437.9320970
GTGGTAT3050.037.639531
GTATCAA19300.037.088871
AAGTTAT16400.036.914470
CCCACTT8800.036.0354970
GGTATCA8600.035.335331
AATTTCA18000.033.6331270
TAATCGT1300.002719797233.26352770
CCCCGTA1750.003250320732.94672870
Produced by FastQC (version 0.11.5)