FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1338745
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCTTCGGCTCTCAGGCTGTTCAGTTGCAGATACA66680.4980784241957953No Hit
GTATAATAGATGAGGACTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTACAGTATTAATTCCGATGT47800.35705081998438837No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAG45520.3400199440520786No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC42470.31723741265140115No Hit
CTATTATACTAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC40510.3025968350955559No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAG39600.29579942408748494No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAG36230.2706265943103429No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG35290.26360509282947836No Hit
GATTATTATTGTGCAGCATGGGATGACAGGCTGAGTGGTTTTGTCTTCGG35200.2629328214110977No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC34060.2544173834449428No Hit
GTATTAATTCCGATGTTGGAGGTGCTTCCAGAACAAGAGATGGTGACCCT33120.24739588196407827No Hit
GTCTATTACTGTGCGAGAGATTGGAGGCCGACTACGGTGACGTACTACTA32720.24440800899349766No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTG31380.23439863454205243No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC28510.21296064597813624No Hit
ATAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG27020.2018308191627233No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACAAAACCACTCAGC26670.19921643031346523No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26430.19742370653111682No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCA26270.19622855734288458No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC25600.19122387011716196No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT25570.1909997796443684No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC25360.1894311463348136No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG24770.1850240337032071No Hit
GAATTAATACTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAA24490.18293252262380066No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACAAAACCACTCAGCCTGTCATCCCA24310.18158797978703936No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC24200.18076631472012966No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG23400.17479056877896837No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23120.1726990576995619No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACAAAACCACTCAGCC21120.15775969284665864No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG20970.15663924048269087No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG20480.15297909609372956No Hit
ATACTAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCA20250.15126106913564571No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG20240.15118637231138118No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT20170.15066349454152958No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT19590.1463310787341876No Hit
GCATGGGATGACAGGCTGAGTGGTTTTGTCTTCGGAACTGGGACCAAGGT19430.14513592954595533No Hit
GTCTGAGGATGAGGCTGATTATTATTGTGCAGCATGGGATGACAGGCTGAGTGGTTTTGTCTTCGGAACTGGGAC19420.14506123272169083No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACT19160.14311911529081342No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC19140.1429697216422844No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT19110.14274563116949082No Hit
GGGCTGGAGTGGGTGGCACTGATTTGGTTTGATGGAAGCAATCAATTTTA19010.14199866292684565No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG18980.14177457245405212No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAG18880.14102760421140695No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGC18780.1402806359687618No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC18580.13878669948347147No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC18150.13557473604009726No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18120.1353506455673037No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG18090.13512655509451016No Hit
GATTAGTATAATAGATGAGGACTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTACAGTATTAATTCCGA17950.13408079955480692No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA17280.12907611232908434No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC17150.1281050536136456No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAG16460.122950972739394No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16420.12265218544233593No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA16370.12227870132101334No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC16360.12220400449674883No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC16050.1198884029445488No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG15770.11779689186514235No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15770.11779689186514235No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA15590.11645234902838104No Hit
CCCCTGAGGGCCGCTGATTAGTATAATAGATGAGGACTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTA15580.11637765220411653No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15420.11518250301588429No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT15420.11518250301588429No Hit
CTCCTGTGCAACGTCTGGATTCACCTTCAGTGCCTTTGACATGCACTGGGTTCGCCAGACTCAAGGCAAGGGG15250.1139126570033875No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT15110.11286690146368426No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC15080.11264281099089071No Hit
CCGTAGTAGTACGTCACCGTAGTCGGCCTCCAATCTCTCGCACAGTAATAGACAGCCGTGTCTTCGGCTCTCAGG14760.1102525126144262No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT14730.11002842214163265No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTATTGTGCAGCATGGGATGACAGGCTGAGTGGTTT14690.10972963484457458No Hit
CAGTAATAGACAGCCGTGTCTTCGGCTCTCAGGCTGTTCAGTTGCAGATACAGTGTATTGTTGGAATTGTCTC14370.10733933646811006No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG14350.10718994281958102No Hit
CTCCAGCCCCTTGCCTTGAGTCTGGCGAACCCAGTGCATGTCAAAGGCAC14340.10711524599531651No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14330.10704054917105199No Hit
ATCCCATGCTGCACAATAATAATCAGCCTCATCCTCAGACTGGAGCCCAC14300.10681645869825844No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA14280.1066670650497294No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA14230.10629358092840682No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG14200.10606949045561327No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC14070.10509843174017458No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG13930.10405267620047134No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTATTGTGCAGCA13920.10397797937620683No Hit
CTACTACGGTATGGACGTCTGGGGCCAGGGGACCACGGTCATCGTCTCCTCAGCATCCCCGACCAGCCCCAAGG13710.10240934606665197No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13540.1011395000541552No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTT13490.10076601593283262No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)