FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1338745
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCTTCGGCTCTCAGGCTGTTCAGTTGCAGATACAGTGTATTGTTGGAATTGTCTCTGGA54100.40410981927103373No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50230.37520214828066584No Hit
GTATAATAGATGAGGACTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTACAGTATTAATTCCGATGTTG45480.33972115675502057No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC43760.32687330298152373No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT43230.3229143712955044No Hit
CTATTATACTAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC41510.3100665175220076No Hit
GTCTATTACTGTGCGAGAGATTGGAGGCCGACTACGGTGACGTACTACTACGGTATGGACGTCTGGGGCCAGGG40540.3028209255683495No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC37230.27809627673679455No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA36500.27264340856548486No Hit
GATTATTATTGTGCAGCATGGGATGACAGGCTGAGTGGTTTTGTCTTCGGAACTGGGACCAAGGTCACCGTCCTA33180.24784406290966537No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC32490.24268998203541375No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGT31690.23671423609425246No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGG30830.23029030920750404No Hit
GTATTAATTCCGATGTTGGAGGTGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGACGCTGAG28650.21400640151783948No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT27840.20795595875241366No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT27350.20429581436345232No Hit
GAATTAATACTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAAGTCCTCATCTATTATACTAATCAGC26770.1999633985561104No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAG24920.18614448606717485No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC24550.18338070356938777No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTA24190.18069161789586516No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC23790.17770374492528448No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGT23220.17344602594220707No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCA23210.17337132911794254No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22710.16963648790471672No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACAAAACCACTCA22240.16612573716428444No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCA21600.16134514041135542No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC21250.15873075156209734No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACAAAACCACTCAGC20750.15499591034887153No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACAAAACCACTCAGCCTGTCATCCCAT19970.14916955805623924No Hit
GGGCTGGAGTGGGTGGCACTGATTTGGTTTGATGGAAGCAATCAATTTTA19250.14379138670919406No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG19210.143492599412136No Hit
ATACTAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG19180.14326850893934245No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGG19040.14222275339963922No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT18640.13923488042905857No Hit
GTCTGAGGATGAGGCTGATTATTATTGTGCAGCATGGGATGACAGGCTGAGTGGTTTTGTCTTCGGAACTGGGAC18580.13878669948347147No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCT18460.13789033759229727No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC18090.13512655509451016No Hit
GATTAGTATAATAGATGAGGACTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTACAGTATTAATTCC17790.13288565036657468No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTT17640.13176519800260691No Hit
GCATGGGATGACAGGCTGAGTGGTTTTGTCTTCGGAACTGGGACCAAGGT17620.13161580435407788No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC17540.13101822975996175No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC17420.13012186786878757No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG17210.1285532345592327No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACAAAACCACTCAGCC16860.12593884570997466No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG16810.12556536158865206No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16810.12556536158865206No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16810.12556536158865206No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16650.12437021240041979No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16470.12302566956365851No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA16320.12190521719969076No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTG16210.12108355213278107No Hit
CTCCAGCCCCTTGCCTTGAGTCTGGCGAACCCAGTGCATGTCAAAGGCACTGAAGGTGAATCCAGACGTTGCACA15970.11929082835043268No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC15790.11794628551367138No Hit
CTCTTGTTCTGGAAGCACCTCCAACATCGGAATTAATACTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCC15680.1171246204467617No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT15670.11704992362249718No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC15640.11682583314970363No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTATTGTGCAG15560.1162282585555875No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC15360.11473432207029718No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAG14940.1115970554511875No Hit
CTCCTGTGCAACGTCTGGATTCACCTTCAGTGCCTTTGACATGCACTGGGTTCGCCAGACTCAAGGCAAGGG14750.11017781579016168No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAG14690.10972963484457458No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14530.10853448565634233No Hit
ATAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGA14500.10831039518354878No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAG14450.10793691106222619No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14410.10763812376516813No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG14330.10704054917105199No Hit
CCCCTGAGGGCCGCTGATTAGTATAATAGATGAGGACTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTA14140.10562130951002617No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCT14040.10487434126738103No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTATTGTGCAGCATG13890.10375388890341328No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA13790.10300692066076811No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG13780.10293222383650359No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGATGCCCAAGTCAGGTACTTCTCGC13700.10233464924238746No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC13670.1021105587695939No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTTGT951.2553888E-463.7785470
AATGGTA2054.037065E-851.7228470
CGATTCA9800.047.9152670
CTCTTGT28150.047.8905570
AAGCGCG306.3730445E-445.90993546
GGTATCA11400.044.9343341
CAGTCAT1357.117004E-444.881270
GTGGTAT3100.043.772821
CTCTGGA17000.042.8564969
GTATCAA30700.041.990541
TCCCAGT29450.041.6617970
CTCTTAA7500.040.39307870
AAGCGGT14000.040.03242570
GAGCCAT14900.039.6475770
TATAATT1550.001404504639.09007670
ACGTTCT350.001449458138.85009830
TGGTATC3500.037.7712972
AATAATA18350.037.14622570
ATATAGC558.354167E-636.9733666
ACCAGGT2509.356327E-636.35376770
Produced by FastQC (version 0.11.5)