FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1385187
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATGGGACAGTAGTCTCAGTGCTTATGTCTTCGGAACTGGGACCACGGT41780.3016199256851241No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCGTGGTCCCAG37200.26855579788144124No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAG36650.26458521484824793No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC35780.2583044744139239No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG34380.24819753578397716No Hit
GTAATACATAGCCGTGTCGTCGTCTCTCAGACTGTCCAACTGCAGAAACA34320.24776438127126518No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC33120.23910129101702512No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAG32130.2319542415572771No Hit
GTCCCATGCTGAGCAATAATAGTCAGCCTCGTCCTCAGGCTGGAGTCCAGTAATGGTCAGGGAGGCTGTGTTTC32080.23159327946335045No Hit
TTACTGGACTCCAGCCTGAGGACGAGGCTGACTATTATTGCTCAGCATGGGACAGTAGTCTCAGTGCTTATGTCT31650.22848900545558107No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC30600.22090880148312106No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC29620.21383394444215836No Hit
ATGTATTACTGTGCGAGGGATGATTGCAGTAGTGCCAGATGCTACACGGCGTTTGACTACTGGGGCCAGGGAACC29610.21376175202337303No Hit
GACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACATAAGCACTGAGA28370.20480989209399164No Hit
CACCTGCAGTGGGAACGGCAACAATGTTGGCAACCAAGGAGCAGCTTGGCTGCAGCAGCGCCAGGGCCACCCTCC27410.19787941989059962No Hit
GGACAGTAGTCTCAGTGCTTATGTCTTCGGAACTGGGACCACGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCC27020.1950639155579716No Hit
ACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACATAAGCACTGAGAC25100.18120297115118755No Hit
GCCTTGGGCTGACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACATAAGCACTGAGACTACTGTCCCA24620.1777377350494915No Hit
CTCCTATTCTATAGGAATAACAACCGGCCCTCAGGGATCTCAGAGAGATT23920.17268426573451814No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23450.1692912220516075No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC23080.16662010255655013No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT22620.16329925129242479No Hit
GGCTTGAGACAGACCGCCACACTCACCTGCAGTGGGAACGGCAACAATGTTGGCAACCAAGGAGCAGCTTGGC21760.1570907032768861No Hit
GTCCAGTAATGGTCAGGGAGGCTGTGTTTCCTGACCTGGATGCAGAGAATCTCTCTGAGATCCCTGAGGGCCGGT21060.1520372339619127No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG20760.14987146139835272No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG19910.14373510580159937No Hit
GTCTGAGAGACGACGACACGGCTATGTATTACTGTGCGAGGGATGATTGCAGTAGTGCCAGATGCTACACGGCGT19360.139764522768406No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCGTGGTC19320.13947575309326468No Hit
CAGTAATACATAGCCGTGTCGTCGTCTCTCAGACTGTCCAACTGCAGAAACAGTGAGTTCTTGGTGTTGTCTC19250.13897040616176734No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGC18940.136732441179422No Hit
GTATTACTGTGCGAGGGATGATTGCAGTAGTGCCAGATGCTACACGGCGTTTGACTACTGGGGCCAGGGAACC18640.13456666861586197No Hit
CTACTGTCCCATGCTGAGCAATAATAGTCAGCCTCGTCCTCAGGCTGGAGTCCAGTAATGGTCAGGGAGGCTGTG18490.13348378233408198No Hit
GACTATTATTGCTCAGCATGGGACAGTAGTCTCAGTGCTTATGTCTTCGG18400.13283405056501396No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG18310.13218431879594597No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG18290.1320399339583753No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC18210.13146239460809261No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT17460.1260479631991926No Hit
GAATAGGAGTTTGGGAGGGTGGCCCTGGCGCTGCTGCAGCCAAGCTGCTCCTTGGTTGCCAACATTGTTGCCGT17240.12445972998591526No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17090.12337684370413526No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT16320.11781802745766455No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16280.11752925778252322No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC16230.11716829568859655No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT16200.11695171843224056No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG16140.11651856391952854No Hit
GGGCTGACTCAGCCACCCTCGGTTTCCAAGGGCTTGAGACAGACCGCCAC16050.11586883215046054No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCTGGCTGTGCCCAGCCA15360.11088755525427253No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC15060.10872178269071252No Hit
ATAATAGTCAGCCTCGTCCTCAGGCTGGAGTCCAGTAATGGTCAGGGAGG15030.10850520543435652No Hit
CCGCCACACTCACCTGCAGTGGGAACGGCAACAATGTTGGCAACCAAGGA14670.10590627835808451No Hit
CTATTATTGCTCAGCATGGGACAGTAGTCTCAGTGCTTATGTCTTCGGAACTGGGACCACGGTCACCGTCCTAGG14390.10388489063209516No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA14390.10388489063209516No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACT14370.10374050579452448No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGAA150.008214871138.0274470
GTTGCGA200.001184414182.0514169
AGGGGGG3450.072.0143270
TTGCGTA300.00590919654.70093569
TCTGGAG16850.052.83542670
GGTATCA11950.044.0434461
GTATCAA30350.038.2179071
TCTATAC451.18991695E-437.228183
TGTACGG451.19016986E-437.22683310
CGTATGC2750.036.18457441
TCGTATG2650.034.90230640
ATGGCAA2756.179107E-933.87946370
AATGGTA5000.033.12658770
TCTCGCG12250.032.95757370
ATCTCGT2600.032.70906437
GAGTTTA3900.032.6639371
TATCAAC35850.032.5228582
ATGCCGT3000.032.19323344
CTTATAC3650.032.1458361
TATCTCG2750.032.11485336
Produced by FastQC (version 0.11.5)