FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1385187
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC37330.2694942993256506No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT36490.26343013614768257No Hit
GCATGGGACAGTAGTCTCAGTGCTTATGTCTTCGGAACTGGGACCACGGT36320.2622028650283319No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35000.25267346574866784No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA34310.24769218885247984No Hit
GTAATACATAGCCGTGTCGTCGTCTCTCAGACTGTCCAACTGCAGAAACAGTGAGTTCTTGGTGTTGTCTCTGGA31060.2242296527472464No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGG30990.2237243058157491No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC30860.22278580437153972No Hit
GTCCCATGCTGAGCAATAATAGTCAGCCTCGTCCTCAGGCTGGAGTCCAGTAATGGTCAGGGAGGCTGTGTTTC30590.22083660906433572No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAG29590.21361736718580235No Hit
ATGTATTACTGTGCGAGGGATGATTGCAGTAGTGCCAGATGCTACACGGCGTTTGACTACTGGGGCCAGGGAACC29390.21217351881009566No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGT28950.208997052383541No Hit
TTACTGGACTCCAGCCTGAGGACGAGGCTGACTATTATTGCTCAGCATGG28680.207047857076337No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC28020.20228315743650496No Hit
GGACAGTAGTCTCAGTGCTTATGTCTTCGGAACTGGGACCACGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCC26590.19195964155020226No Hit
CTCCTATTCTATAGGAATAACAACCGGCCCTCAGGGATCTCAGAGAGATTCTCTGCATCCAGGTCAGGAAACACA25700.18553451627830755No Hit
CACCTGCAGTGGGAACGGCAACAATGTTGGCAACCAAGGAGCAGCTTGGCTGCAGCAGCGCCAGGGCCACCCTCC25420.1835131285523182No Hit
GACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACATAAGCACTGAGA24110.1740559216914395No Hit
ACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACATAAGCACTGAGACTACTGTCCCATGCTGAGCAATAATA24080.1738393444350835No Hit
GCCTTGGGCTGACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACATAAGCACTGAGACTACTGTCCCAT22740.16416556031784876No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22560.16286609677971275No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC22030.1590398985840901No Hit
GTCCAGTAATGGTCAGGGAGGCTGTGTTTCCTGACCTGGATGCAGAGAAT21680.15651316392660342No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTA21650.1562965866702474No Hit
GGCTTGAGACAGACCGCCACACTCACCTGCAGTGGGAACGGCAACAATGTTGGCAACCAAGGAGCAGCTTGGCT20850.1505211931674207No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20730.14965488414199674No Hit
GTCTGAGAGACGACGACACGGCTATGTATTACTGTGCGAGGGATGATTGC19310.13940356067447934No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGT19190.1385372516490553No Hit
GGGCTGACTCAGCCACCCTCGGTTTCCAAGGGCTTGAGACAGACCGCCAC18160.13110143251416595No Hit
CTACTGTCCCATGCTGAGCAATAATAGTCAGCCTCGTCCTCAGGCTGGAG18140.13095704767659527No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG18030.13016293106995663No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTG17900.1292244296257473No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACATAAGCACTGA17820.1286468902754646No Hit
GACTATTATTGCTCAGCATGGGACAGTAGTCTCAGTGCTTATGTCTTCGGAACTGGGACCACGGTCACCGTCCTA17700.1277805812500406No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC17650.12741961915611394No Hit
GTATTACTGTGCGAGGGATGATTGCAGTAGTGCCAGATGCTACACGGCGTTTGACTACTGGGGCCAGGGAACC17580.1269142722246166No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGG17390.12554261626769528No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16920.12214957258478458No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAG16820.12142764839693124No Hit
GAATAGGAGTTTGGGAGGGTGGCCCTGGCGCTGCTGCAGCCAAGCTGCTCCTTGGTTGCCAACATTGTTGCCGTT16750.12092230146543391No Hit
CAGTAATACATAGCCGTGTCGTCGTCTCTCAGACTGTCCAACTGCAGAAACAGTGAGTTCTTGGTGTTGTCTCTG16720.12070572420907792No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCT16000.11550787005653387No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTT15600.11262017330512053No Hit
GTGCTTATGTCTTCGGAACTGGGACCACGGTCACCGTCCTAGGTCAGCCC15160.10944370687856586No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAG14870.10735012673379117No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGCAGGGCACAGTCACATCCTGGCTGG14860.10727793431500583No Hit
CTCCTGTGTAGCCTCTGGATTCACCTTCAGTGATTATTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGG14710.10619504803322584No Hit
GTGGGAATCTGCACCATGCCCTGGGCTCTGCTCCTCCTGACCCTCCTCACTCACTCTGCAGTGTCAGTGGTCCAG13950.10070842420554046No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)