FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences593449
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTGATTTGCAGATATA38300.6453798051728119No Hit
GTCTATTACTGTGCGAGAGGATCTTGTAGTGGTGGTCTCTGCTACGAGTCCAGAGGATCATACTACTTTGACTA21320.35925580799698037No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT16640.280394776973253No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14930.2515801694838141No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGG13790.23237043115752154No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11880.20018569413715417No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT11670.1966470581296792No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10380.17490972265519025No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10210.172045112553901No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT10160.17120258017116888No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9810.16530485349204396No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT9590.1615977110080226No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9500.16008115271910475No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTTAGTGGCAGTGGATCTGGGACAGATTTCA9410.15856459443018692No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATCAGCACCTATTTAAATTGGTATCTGCAGAAA8940.15064479003250492No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTAAGTTATTATACCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG8910.1501392706028656No Hit
TATGTACACTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGACACAACGGCAAGAACTCACTATATC8880.14963375117322636No Hit
ACCTACTACTGTCAACAGAGTTTCATTAGTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA8870.14946524469667993No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8850.14912823174358705No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8760.14761167345466925No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC8500.14323050506446217No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8490.14306199858791574No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8460.14255647915827643No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8450.14238797268173004No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTCCTTGTTGCTATTTTAGAAGG8320.1401973884866265No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG8280.13952336258044076No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8080.13615323304951224No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATCAGCAC8060.13581622009641942No Hit
GTCCATGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTATGATCCTC8010.13497368771368728No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT7960.13413115533095513No Hit
CAGTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTGATTTGCAGATATAGTGAGTTCTTGCCGTTGTGTC7910.13328862294822302No Hit
GTAGTAGGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGA7860.1324460905654909No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA7760.13076102580002663No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7760.13076102580002663No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC7670.1292444675111088No Hit
CCATGGAACTGGGGCTCCGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGTTGG7610.12823342865183024No Hit
GCCTTAGCCCTGGATTCCAAGGCCTATCCACTTGGTGATCAGCACTGAGC7540.12705388331600526No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG7510.12654836388636598No Hit
GGATCATACTACTTTGACTACTGGGGCCAGGGAACCCTGGTCATGGACTC7510.12654836388636598No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7460.12570583150363385No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC7180.12098765016033391No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG7140.12031362425414821No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT7120.11997661130105534No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT7080.11930258539486965No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATG6930.11677498824667326No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGACTAA6850.11542693643430185No Hit
CTACAAGATCCTCTCGCACAGTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTGATTTGCAGATATAGTGAG6830.115089923481209No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC6810.11475291052811613No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC6720.11323635223919831No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC6710.11306784576265189No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC6560.11054024861445551No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC6430.10834966441935195No Hit
CTACTACTGTCAACAGAGTTTCATTAGTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTG6340.10683310613043412No Hit
GCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCAGATACCAATTTAAATAGGTGCTGATGCTCTGACT6320.10649609317734128No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA6300.10615908022424841No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG6300.10615908022424841No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACA6280.10582206727115556No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG6210.10464252193533058No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC6180.10413700250569131No Hit
CTATTACTGTGCGAGAGGATCTTGTAGTGGTGGTCTCTGCTACGAGTCCAGAGGATCATACTACTTTGACTAC6170.10396849602914487No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC6170.10396849602914487No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACCTACTACTGTCAACAGAGTTTCATTAG6160.10379998955259845No Hit
GTCTGGGGGAGGCCTGGTCAAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTAAGTTA6110.10295745716986632No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT6100.10278895069331989No Hit
GTATGATCCTCTGGACTCGTAGCAGAGACCACCACTACAAGATCCTCTCG6090.10262044421677347No Hit
CTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGACACAACGGCAAGAACTCACTATATCTGCAAATCAA6060.1021149247871342No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT6050.10194641831058777No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC6040.10177791183404133No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6040.10177791183404133No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5990.10093537945130922No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)