FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences593449
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31390.5289418298792314No Hit
GTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTGATTTGCAGATATAGTGAGTTCTTGCCGTTGTGTCTGGA30990.5222015708173744No Hit
GTCTATTACTGTGCGAGAGGATCTTGTAGTGGTGGTCTCTGCTACGAGTCCAGAGGATCATACTACTTTGACTA21460.36161489866863034No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16530.27854120573124225No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA15070.2539392601554641No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13110.22091199075236456No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT10320.1738986837959117No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10210.172045112553901No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTAAGTTATTATACCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGG9630.1622717369142083No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG9390.15822758147709406No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC9100.1533408936572477No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8700.1466006345953907No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC8700.1466006345953907No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTTAGTGGCAGTGGATCTGGGACAGATTTCA8670.14609511516575138No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG8670.14609511516575138No Hit
ACCTACTACTGTCAACAGAGTTTCATTAGTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA8580.14457855687683355No Hit
TATGTACACTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGACACAACGGCAAGAACTCACTATATCT8370.14103992086935863No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8290.13969186905698722No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC8230.13868083019770866No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATCAGCACCTATTTAAATTGGTATCTGCAGAAA8190.13800680429152296No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8180.13783829781497653No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA8170.1376697913384301No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC8150.13733277838533722No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8140.1371642719087908No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8040.13547920714332656No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7830.1319405711358516No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA7710.12991849341729447No Hit
GTCCATGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTATGATCCTC7620.12840193512837667No Hit
GTAGTAGGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGA7410.12486329912090172No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGT7320.12334674083198387No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG7300.12300972787889103No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG7220.12166167606651962No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC7090.11947109187141608No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG7070.11913407891832321No Hit
CCATGGAACTGGGGCTCCGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGTTGGTG6930.11677498824667326No Hit
CAGTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTGATTTGCAGATATAGTGAGTTCTTGCCGTTGTGTCTG6880.11593245586394114No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATCAGCAC6750.11374187166883758No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACCTACTACTGTCAACAGAGTTTCATTAGT6750.11374187166883758No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA6660.11222531337991976No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT6550.11037174213790907No Hit
CTACTACTGTCAACAGAGTTTCATTAGTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGT6540.11020323566136264No Hit
GGATCATACTACTTTGACTACTGGGGCCAGGGAACCCTGGTCATGGACTCCTCAGCATCCCCGACCAGCCCCAAG6530.11003472918481622No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGACTAAT6400.10784414498971268No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGTCCATGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGTAGTAT6400.10784414498971268No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG6380.10750713203661984No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6350.10700161260698055No Hit
GCCTTAGCCCTGGATTCCAAGGCCTATCCACTTGGTGATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGG6330.10666459965388769No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC6320.10649609317734128No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT6310.10632758670079485No Hit
GTCTGGGGGAGGCCTGGTCAAGCCTGGGGGGTCCCTGAGACTCTCCTGTG6270.10565356079460914No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6250.1053165478415163No Hit
CTACAAGATCCTCTCGCACAGTAATAGACAGCCGTGTCCTCGGCTCTCAG6190.10430550898223774No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAGGCTCAGCGGGAAGACCTTGGG6130.10329447012295918No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC6100.10278895069331989No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAG6090.10262044421677347No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTA6010.10127239240440207No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)