FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1333364
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAG51280.3845911544034487No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAG40180.3013430691094105No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC38340.2875433865021104No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC30730.23046969919691848No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACTGAGGACGGCAACCTTAGTCCCA28370.21277010628755538No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC27310.20482028913334996No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC26530.19897042368025533No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24890.1866707065737488No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24380.18284579454672542No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT24120.1808958393956939No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG24080.1805958462955352No Hit
GATTATTATTGTTCTTCACTTTCAACCAGTAACACCCTTCCTTATATTTTTGGAAGTGGGACTAAGGTTGCCGTC23990.17992086182017814No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG22350.1676211447136716No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT22330.16747114816359224No Hit
GAAATATATCGCCGTGTCATCGGACTTAAGGCCGGCGAGGTCCATGTAAACTGTACTTGCGGATGTGTCGCGGG21850.16387123096168787No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21710.16282125511113246No Hit
TCTCTGGACTCCAGGCTGAAGACGACGCTGATTATTATTGTTCTTCACTTTCAACCAGTAACACCCTTCCTTATA21140.15854635343387102No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTGCCACTGCTCGGGCGTCAGGCTCAGGTAG21120.15839635688379167No Hit
GGACTAAGGTTGCCGTCCTCAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAG20970.15727138275819655No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACT20060.15044653972958622No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACTGAGGACGGCAACCTTAGTCCCACTTCCAAAAATATAAGGAAGGG19840.1487965776787134No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19510.14632163460240416No Hit
GTCTGGGGCTCAGGTGAAGAAGCCGGGGGCCTCAGTGAGGATCTCCTGTAAGACCTCCGGATACAGCTTCATAAG19450.14587164495216612No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCC19000.14249672257538076No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTGCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGC18910.14182173810002371No Hit
GCCTTGGGCTGACTGAGGACGGCAACCTTAGTCCCACTTCCAAAAATATAAGGAAGGGTGTTACTGGTTGAAAG18860.14144674672482532No Hit
GTCATTCCCTTTCGACTCCTGGGGCCAGGGATCCCTGGTCACTGTCTCCGCAGCATCCCCGACCAGCCCCAAGG18640.1397967846739525No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT18540.13904680192355576No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18340.13754683642276228No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC18330.1374718381477226No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG18280.13709684677252423No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17780.13334693302054051No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG17650.13237195544502475No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG17450.13087198994423127No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT17450.13087198994423127No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA16610.12457213484089866No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCATTGGAACCAGTAATGATATT16360.12269717796490681No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTGCCACTGCTCGGGCGTCAGGCTCAGGTAGC16310.12232218658970843No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG16190.12142220728923235No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC16080.12059722626379593No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA15840.11879726766284375No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG15840.11879726766284375No Hit
GTAACACCCTTCCTTATATTTTTGGAAGTGGGACTAAGGTTGCCGTCCTCAGTCAGCCCAAGGCCAACCCCA15730.11797228663740734No Hit
CCTTAGTCCCACTTCCAAAAATATAAGGAAGGGTGTTACTGGTTGAAAGTGAAGAACAATAATAATCAGCGTCG15480.1160973297614155No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG15360.11519735046093939No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG15300.11474736081070135No Hit
GTGTTACTGGTTGAAAGTGAAGAACAATAATAATCAGCGTCGTCTTCAGC15300.11474736081070135No Hit
GGGAATGACCGTGCACAGAAATATATCGCCGTGTCATCGGACTTAAGGCCGGCGAGGTCCATGTAAACTGTACT15110.11332239358494754No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC15080.11309739875982852No Hit
ATATATTTCTGTGCACGGTCATTCCCTTTCGACTCCTGGGGCCAGGGATCCCTGGTCACTGTCTCCGCAGCATCC15060.11294740220974916No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC14550.10912249018272581No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG14520.10889749535760676No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC14480.10859750225744808No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC14470.10852250398240841No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT14350.10762252468193231No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG14230.10672254538145623No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGCAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCG14130.10597256263105949No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA14070.10552257298082145No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC13700.10274763680435349No Hit
ACTCCTTACCATGGACTGGACCTGGAGGATCCTCTTTTTGGTGGCAGCAG13690.10267263852931383No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13520.10139766785363938No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCATTGGAACCAGTAATGATAT13480.10109767475348067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2550.080.76517570
GTTTATA500.002976643465.03722470
CGTATGG252.6395306E-454.890941
GTGGTAT3350.050.591311
TGGTATC3650.046.408742
CGCGTAT451.9635172E-645.64534439
ATCGCGT405.300903E-542.70350637
GGTATCA10050.038.4493941
ACGAGAC2950.037.91064522
TATACAC2950.037.9006923
CTCTTAT1100.036.9776461
TGGGTAG4150.036.56711270
GTATCAA23500.036.348241
GACAGGT2250.036.155921
TCGTATG1051.8189894E-1135.9063840
CACGAGA3050.035.55386421
CGAGACT3300.034.9207123
TACACAT3300.034.9089245
TTATACA3300.034.9049952
GCGTATG601.4044292E-534.27427340
Produced by FastQC (version 0.11.5)