FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1333364
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT46490.34866698065944485No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC39900.2992431174082996No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37390.2804185503733414No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGT36580.2743436900951278No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC28130.21097014768660322No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC26360.19769545300458088No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC26130.1959704926786684No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACTGAGGACGGCAACCTTAGTCCCA25520.19139559790124827No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23890.1791708790697814No Hit
GATTATTATTGTTCTTCACTTTCAACCAGTAACACCCTTCCTTATATTTTTGGAAGTGGGACTAAGGTTGCCGTC23200.17399599809204389No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGG22500.1687461188392667No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGG22040.1652961981874417No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTGCCACTGCTCGGGCGTCAGGCTCAGGTA21970.16477121026216396No Hit
TCTCTGGACTCCAGGCTGAAGACGACGCTGATTATTATTGTTCTTCACTT21710.16282125511113246No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGT21650.1623712654608944No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC21560.16169628098553734No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC20710.15532142760716502No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20670.15502143450700634No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20370.1527714862558161No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG19970.14977155525422917No Hit
GTCTGGGGCTCAGGTGAAGAAGCCGGGGGCCTCAGTGAGGATCTCCTGTA19590.14692162080272153No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC19410.1455716518520074No Hit
GGACTAAGGTTGCCGTCCTCAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGA19200.14399668807617425No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCT19020.14264671912546012No Hit
GAAATATATCGCCGTGTCATCGGACTTAAGGCCGGCGAGGTCCATGTAAACTGTACTTGCGGATGTGTCGCGGGT18980.1423467260253014No Hit
GCCTTGGGCTGACTGAGGACGGCAACCTTAGTCCCACTTCCAAAAATATAAGGAAGGGTGTTACTGGTTGAAAGT17960.13469690197125467No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTGCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTG17750.1331219381954215No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17630.13222195889494542No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17110.12832204859288238No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG16890.12667208654200954No Hit
GTCATTCCCTTTCGACTCCTGGGGCCAGGGATCCCTGGTCACTGTCTCCGCAGCATCCCCGACCAGCCCCAAGGT16690.12517212104121606No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTT16560.12419714346570028No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACTGAGGACGGCAACCTTAGTC16460.12344716071530355No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC16380.12284717451498615No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTGCCACTGCTCGGGCGTCAGGCTCAGGTAGCT16330.12247218313978779No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16330.12247218313978779No Hit
GGGTGTTACTGGTTGAAAGTGAAGAACAATAATAATCAGCGTCGTCTTCAGCCTGGAGTCCAGAGATTGTCAGG16250.12187219693947039No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCATTGGAACCAGTAATGATATT16060.12044722971371659No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG15680.11759729526220897No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14900.11174742980911438No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAG14860.1114474367089557No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC14780.1108474505086383No Hit
GGGAATGACCGTGCACAGAAATATATCGCCGTGTCATCGGACTTAAGGCCGGCGAGGTCCATGTAAACTGTACTT14490.10867250053248774No Hit
GTAACACCCTTCCTTATATTTTTGGAAGTGGGACTAAGGTTGCCGTCCTCAGTCAGCCCAAGGCCAACCCCA14440.10829750915728939No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC14320.10739752985681329No Hit
ATATATTTCTGTGCACGGTCATTCCCTTTCGACTCCTGGGGCCAGGGATCCCTGGTCACTGTCTCCGCAGCATCC14170.10627255573121819No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA14150.10612255918113883No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCT14060.10544757470578178No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG14040.10529757815570243No Hit
GTGTTACTGGTTGAAAGTGAAGAACAATAATAATCAGCGTCGTCTTCAGCCTGGAGTCCAGAGATTGTCAGGGAG14020.10514758160562307No Hit
GGGTGATGTTGGTACCAGGAGACAAGGTCATAATATCCAATATCATTACTGGTTCCAATGCAGGAGATGGT13910.10432260058018665No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTTCAGGGCCAGCTCCTCCGACGGCGGC13720.10289763335443285No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGCAGTCCCACAGAAGCTACAGCTGC13650.10237264542915514No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG13610.10207265232899643No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC13490.10117267302852036No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC13490.10117267302852036No Hit
CCTTAGTCCCACTTCCAAAAATATAAGGAAGGGTGTTACTGGTTGAAAGTGAAGAACAATAATAATCAGCGTCGT13350.1001226971779649No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCGT754.6234905E-578.0310770
AATGGTA4250.058.52330870
CCGTACG250.00856059549.80718268
GGTATCA7900.043.2647551
GTAGATA1705.0575953E-543.0318470
GTATCAA21150.040.3208961
GAGCCAT16500.039.90225270
TTACTTT2603.4364894E-739.39068670
TGGTATC2150.039.318722
GTGTATA702.5956979E-838.6751481
GTGGTAT2150.037.7757231
CTCGTAT1951.6985723E-837.51494270
GTAGTTT1951.1353185E-437.51494270
TCTTGTT3602.917659E-936.57706570
CTTATAT22750.035.3712370
CGGCAAA1700.002621046334.42547270
GAAGGGT17300.033.828570
CATAAGT17850.032.78616370
GGCGTCA28400.032.45570870
ATCAACG26350.032.2088053
Produced by FastQC (version 0.11.5)