FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences860421
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT41800.48580869132668775No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41730.484995136101978No Hit
GTAATAGACGGCCGTGTCCGCAGCGGTCACAGAGGTCACCTTCAGGGAGAACTGGTTCTTGGACGTGTCTACTG38190.44385248616665557No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG27250.31670542676201535No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27030.3141485389129275No Hit
GTCTCTGATGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCC26810.3115916510638397No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA26260.3051994314411201No Hit
GTCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCGACTGCCGCTGAACCTTGATGGGAC25160.29241499219568096No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22890.2660325584800929No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG22630.26301078193117094No Hit
GTCTATTACTGTGCGAGAGTAAACGAGCAGGGGCTGCCCCGTTCGTATTACTACTACATGGACTTCTGGGGCAA22010.25580500708374154No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG21600.2510398979104415No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT21520.2501101205107732No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21490.2497614539858976No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC21190.24627478873714145No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA21000.24406656741292926No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG20620.2396501247645048No Hit
GTCTCCATCCTTCCTATCTGCATCTATAGGAGACAGAATCACCATCACTT20070.23325790514178527No Hit
GCCTGAAGACTCTGCAACTTATTACTGTCAACAACTTAATATTTTCCCTT19940.23174701686732424No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18830.21884635544692654No Hit
GATCTATGGTGCATCTACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTCGATCTGGGACAGAATTC18650.21675435629767287No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGGTGTGACATCC18460.21454613497346067No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18200.2115243584245387No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCC17980.20896747057545084No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17180.19966969657876782No Hit
CCATAGATCAGGAGGTTAGGGGCTCTCCCGGGTGTTTGTTGATACCAGGC17110.1988561413540581No Hit
ACTTATTACTGTCAACAACTTAATATTTTCCCTTGGACGTTCGGCCAAGGGACCAGGGTGGAGATCAAACGA16760.19478836523050924No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGGTGTGACAT16520.19199903303150434No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC16270.18909347865754092No Hit
GTAATAAGTTGCAGAGTCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCGACTGCCG16060.1866528129834116No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC16010.18607170210861892No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15950.1853743690588677No Hit
ACTTAATATTTTCCCTTGGACGTTCGGCCAAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCAT15780.18339859208457257No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTTGGCCGAACGTCCAAGGGAAAATATTAAGTTGTTGAC15530.18049303771060912No Hit
ATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGACT15300.17781992768656274No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC15180.1764252615870603No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTTGGCCGAACGTCCAAGGGAAAAT15080.1752630398374749No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC15010.17444948461276516No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14960.17386837373797245No Hit
GGTTAGGGGCTCTCCCGGGTGTTTGTTGATACCAGGCTAAAGAATTGCTA14660.17038170848921633No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14540.16898704238971388No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14400.16735993194029433No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13430.15608638096931618No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13340.15504038139468934No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13320.15480793704477228No Hit
CACCTGCACTGTCTCTGATGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGG13120.1524834935456015No Hit
GGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG13040.15155371614593321No Hit
GTAGATGCACCATAGATCAGGAGGTTAGGGGCTCTCCCGGGTGTTTGTTGATACCAGGCTAAAGAATTGCTAATG12670.14725349567246732No Hit
GTGCATCTACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTCGA12660.14713727349750877No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12630.14678860697263316No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12590.14632371827279902No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGGTGTGACATCCAGTTGACCCAGTCTCCATCCTTCCT12450.14469660782337948No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12410.14423171912354532No Hit
GGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGA12370.1437668304237112No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGGTGTGACATCCAGTTG12340.14341816389883558No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12270.1426046086741258No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGACTCTGCAACTTATTACTGTCAACAACTTAATATTTTC12140.1410937203996648No Hit
GTGTGACATCCAGTTGACCCAGTCTCCATCCTTCCTATCTGCATCTATAG12020.13969905430016236No Hit
ACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTCGATCTGGGACAGAATTCACTCTCACAATCAGCAGC12000.1394666099502453No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11980.13923416560032822No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11930.13865305472553552No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11870.1379557216757843No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11700.13597994470148916No Hit
CCCTGGTCCCTTGGCCGAACGTCCAAGGGAAAATATTAAGTTGTTGACAGTAATAAGTTGCAGAGTCTTCAGG11470.13330683467744278No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11410.13260950162769156No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT10980.12761194810447443No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10930.12703083722968175No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10930.12703083722968175No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA10850.12610105983001343No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT10810.1256361711301793No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10720.12459017155555245No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10690.12424150503067685No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAA10670.12400906068075977No Hit
GCCCCTAACCTCCTGATCTATGGTGCATCTACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTCGAT10660.12389283850580123No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10640.12366039415588415No Hit
CAGTAATAGACGGCCGTGTCCGCAGCGGTCACAGAGGTCACCTTCAGGGAGAACTGGTTCTTGGACGTGTCT10590.12307928328109147No Hit
TCTCCACCCTGGTCCCTTGGCCGAACGTCCAAGGGAAAATATTAAGTTGTTGACAGTAATAAGTTGCAGAGTCTT10540.12249817240629877No Hit
ATAGGAGACAGAATCACCATCACTTGCCGGGCCAGTCAGGTCATTAGCAATTCTTTAGCCTGGTATCAACAAAC10430.12121972848175486No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10200.1185466184577085No Hit
CCCTTGGACGTTCGGCCAAGGGACCAGGGTGGAGATCAAACGAACTGTGG10020.11645461930845481No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9980.11598973060862067No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT9950.11564106408374504No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGAAATCCCTTTGCCCCAGAAGTCCATGTAGTAGTAAT9860.1145950645091182No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9860.1145950645091182No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC9840.11436262015920114No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT9780.1136652871094499No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC9750.11331662058457428No Hit
ATATTAAGTTGTTGACAGTAATAAGTTGCAGAGTCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC9730.11308417623465722No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9700.11273550970978162No Hit
CACCCGGGAGAGCCCCTAACCTCCTGATCTATGGTGCATCTACTTTACAA9690.11261928753482307No Hit
CCCCTAACCTCCTGATCTATGGTGCATCTACTTTACAAAGTGGGGTCCCA9690.11261928753482307No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9680.11250306535986453No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9660.11227062100994745No Hit
ACCTTGATGGGACCCCACTTTGTAAAGTAGATGCACCATAGATCAGGAGG9640.11203817666003038No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG9610.11168951013515477No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC9590.1114570657852377No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9550.11099217708540354No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC9500.11041106621061086No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG9350.10866773358623279No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACG9350.10866773358623279No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATC9310.10820284488639864No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9260.10762173401160594No Hit
GGTCATTAGCAATTCTTTAGCCTGGTATCAACAAACACCCGGGAGAGCCCCTAACCTCCTGATCTATGGTGCAT9250.1075055118366474No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC9220.1071568453117718No Hit
ATCAGAGACAGTGCAGGTGAGGGACAGGGTCTCCGAAGACTTCACCAGTC9060.1052972905124352No Hit
CCTTCAGGGAGAACTGGTTCTTGGACGTGTCTACTGACATGGTGACTCGA8890.10332151353814004No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCC8850.1028566248383059No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTC8820.10250795831343029No Hit
GATCAGGAGGTTAGGGGCTCTCCCGGGTGTTTGTTGATACCAGGCTAAAG8760.10181062526367905No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT8760.10181062526367905No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC8760.10181062526367905No Hit
CATCAAGGTTCAGCGGCAGTCGATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGACTCTG8750.10169440308872052No Hit
GTTCAGCGGCAGTCGATCTGGGACAGAATTCACTCTCACAATCAGCAGCC8720.10134573656384492No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCAC8710.10122951438888637No Hit
GGAGTGGATTGGCCAAATCTTTTACAATGGGAACACCTACCTCAGCCCCTCCCTCAAAAGTCGAGTCACCATGTC8690.10099707003896928No Hit
GGACTGGAGTGGATTGGCCAAATCTTTTACAATGGGAACACCTACCTCAGCCCCTCCCTCAAAAGTCGAGTCACC8680.10088084786401075No Hit
GTCCCAGATCGACTGCCGCTGAACCTTGATGGGACCCCACTTTGTAAAGTAGATGCACCATAGATCAGGAGGTT8670.10076462568905221No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT8630.10029973698921807No Hit
CTATTACTGTGCGAGAGTAAACGAGCAGGGGCTGCCCCGTTCGTATTACT8620.10018351481425954No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGTT408.700946E-488.6573270
GTGGTAT1350.057.741961
TGGATTG14300.056.21162470
CCCAATT650.00596552854.5583570
TGAGCTT650.00596552854.5583570
CCCGTAT200.00719469652.04521650
CCGTCGT200.00729339951.86618847
CGTATGC2000.041.22157741
ACGAGAC2150.041.03101722
TGAGCGG2609.713403E-1040.91876270
TCTCGCG6700.040.57946870
CTAGCGC2350.040.53041528
ACTAGCG2350.040.5280527
TAGCGCT2400.039.69761329
AGACTAG2400.039.6259225
GAGACTA2400.039.60515624
TCGTATG2000.039.4621640
CGAGACT2500.039.37196423
CACGAGA2350.038.97374721
GTCGTAA350.001438206438.91016830
Produced by FastQC (version 0.11.5)