FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences860421
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39680.46116959023547777No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT36270.42153782857461636No Hit
GTAATAGACGGCCGTGTCCGCAGCGGTCACAGAGGTCACCTTCAGGGAGAACTGGTTCTTGGACGTGTCTACTG34890.4054991684303381No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA25850.3004343222678201No Hit
GTCTCTGATGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCC25690.29857476746848344No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25200.2928798808955151No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG24440.2840469955986662No Hit
GTCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCGACTGCCGCTGAACCTTGATGGGAC24310.28253610732420525No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24140.2805603303499101No Hit
GTCTATTACTGTGCGAGAGTAAACGAGCAGGGGCTGCCCCGTTCGTATTACTACTACATGGACTTCTGGGGCAA22860.26568389195521724No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG21680.25196967531010983No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC21480.24964523181093906No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21020.24429901176284632No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20830.2420907904386341No Hit
GCCTGAAGACTCTGCAACTTATTACTGTCAACAACTTAATATTTTCCCTT20200.23476879341624624No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT19780.22988746206798766No Hit
GATCTATGGTGCATCTACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTCGATCTGGGACAGAATTC19690.22884146249336082No Hit
GTCTCCATCCTTCCTATCTGCATCTATAGGAGACAGAATCACCATCACTT19480.2264007968192315No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA19300.22430879766997783No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18920.21989235502155338No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG18480.21477857932337774No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCC17990.2090836927504094No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGGTGTGACATCC16400.1906043669320019No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG16270.18909347865754092No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16190.1881637012578726No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16100.18711770168324576No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC16070.18676903515837012No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC15550.1807254820605262No Hit
CCATAGATCAGGAGGTTAGGGGCTCTCCCGGGTGTTTGTTGATACCAGGC15530.18049303771060912No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15410.17909837161110667No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGGTGTGACAT15300.17781992768656274No Hit
GGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG15270.17747126116168713No Hit
ACTTATTACTGTCAACAACTTAATATTTTCCCTTGGACGTTCGGCCAAGGGACCAGGGTGGAGATCAAACGA15230.17700637246185297No Hit
ATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGACT15220.17689015028689445No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC15070.17514681766251636No Hit
ACTTAATATTTTCCCTTGGACGTTCGGCCAAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCAT15000.1743332624378066No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTTGGCCGAACGTCCAAGGGAAAAT14910.17328726286317978No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14740.17131148588888462No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC14450.16794104281508704No Hit
GTAATAAGTTGCAGAGTCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCGACTGCCG14270.16584904366583336No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTTGGCCGAACGTCCAAGGGAAAATATTAAGTTGTTGAC14190.16491926626616504No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13250.1539943818200625No Hit
CACCTGCACTGTCTCTGATGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGG13230.15376193747014544No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGACTCTGCAACTTATTACTGTCAACAACTTAATATTTTC13210.15352949312022834No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12850.149345494821721No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12840.14922927264676247No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12590.14632371827279902No Hit
GTGCATCTACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTCGA12520.14551016304808925No Hit
GTGTGACATCCAGTTGACCCAGTCTCCATCCTTCCTATCTGCATCTATAG12450.14469660782337948No Hit
GGTTAGGGGCTCTCCCGGGTGTTTGTTGATACCAGGCTAAAGAATTGCTA12340.14341816389883558No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12190.1416748312744575No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12090.14051260952487213No Hit
GTAGATGCACCATAGATCAGGAGGTTAGGGGCTCTCCCGGGTGTTTGTTGATACCAGGCTAAAGAATTGCTAATG11810.13725838862603307No Hit
GGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGA11770.13679349992619894No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT11730.13632861122636475No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGGTGTGACATCCAGTTG11650.13539883382669649No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11550.13423661207711107No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11380.13226083510281594No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC11250.13074994682835495No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11230.13051750247843788No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGGTGTGACATCCAGTTGACCCAGTCTCCATCCTTCCT11080.12877416985405982No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC10860.12621728200497198No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10840.12598483765505492No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAA10830.12586861548009637No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10800.12551994895522076No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT10780.1252875046053037No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10750.12493883808042806No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA10480.12180083935654756No Hit
ACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTCGATCTGGGACAGAATTCACTCTCACAATCAGCAGC10450.12145217283167194No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10420.12110350630679632No Hit
GCCCCTAACCTCCTGATCTATGGTGCATCTACTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTCGAT10360.12040617325704508No Hit
GTCCCAGATCGACTGCCGCTGAACCTTGATGGGACCCCACTTTGTAAAGTAGATGCACCATAGATCAGGAGGTT10220.11877906280762557No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACG10160.11808172975787433No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10130.11773306323299873No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTCGATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTG10110.11750061888308166No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10050.11680328583333043No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9950.11564106408374504No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG9910.11517617538391091No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9860.1145950645091182No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9820.11413017580928406No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC9820.11413017580928406No Hit
CCCTGGTCCCTTGGCCGAACGTCCAAGGGAAAATATTAAGTTGTTGACAGTAATAAGTTGCAGAGTCTTCAGG9740.11320039840961575No Hit
GGTCATTAGCAATTCTTTAGCCTGGTATCAACAAACACCCGGGAGAGCCCCTAACCTCCTGATCTATGGTGCAT9680.11250306535986453No Hit
TCTCCACCCTGGTCCCTTGGCCGAACGTCCAAGGGAAAATATTAAGTTGTTGACAGTAATAAGTTGCAGAGTCTT9640.11203817666003038No Hit
ATAGGAGACAGAATCACCATCACTTGCCGGGCCAGTCAGGTCATTAGCAATTCTTTAGCCTGGTATCAACAAAC9580.11134084361027914No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9560.11110839926036208No Hit
CCCCTAACCTCCTGATCTATGGTGCATCTACTTTACAAAGTGGGGTCCCA9410.10936506663598401No Hit
CACCCGGGAGAGCCCCTAACCTCCTGATCTATGGTGCATCTACTTTACAA9370.10890017793614985No Hit
ATATTAAGTTGTTGACAGTAATAAGTTGCAGAGTCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC9280.10785417836152301No Hit
CAGTAATAGACGGCCGTGTCCGCAGCGGTCACAGAGGTCACCTTCAGGGAGAACTGGTTCTTGGACGTGTCT9250.1075055118366474No Hit
GGACTGGAGTGGATTGGCCAAATCTTTTACAATGGGAACACCTACCTCAGCCCCTCCCTCAAAAGTCGAGTCACC9180.10669195661193763No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCC9110.10587840138722789No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9050.10518106833747665No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC9040.10506484616251811No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT8980.10436751311276689No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATC8950.10401884658789128No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8810.10239173613847176No Hit
CCCTTGGACGTTCGGCCAAGGGACCAGGGTGGAGATCAAACGAACTGTGG8800.10227551396351323No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8780.10204306961359613No Hit
ACCTTGATGGGACCCCACTTTGTAAAGTAGATGCACCATAGATCAGGAGG8700.10111329221392784No Hit
GGAGTGGATTGGCCAAATCTTTTACAATGGGAACACCTACCTCAGCCCCTCCCTCAAAAGTCGAGTCACCATGTC8680.10088084786401075No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC8630.10029973698921807No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)