FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1330732
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGTCAGCAGTATGGTACCGAACCTCGCACTTTCGGCGGAGGGACCAAGGTGGAGACCAAACGAACTG58160.43705268979779544No Hit
GTATGGTACCGAACCTCGCACTTTCGGCGGAGGGACCAAGGTGGAGACCAAACGAACTGTGGCTGCACCA42490.3192979503010373No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT38390.2884878397754018No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35830.2692503073496391No Hit
GTACCATACTGCTGACAATAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC35810.26910001412756285No Hit
CAATAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA32390.24339987315252057No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGCTTTGT25740.19342737681216052No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCATCCTCCTGCTACTCTGGCTCCCAGATCCCA25090.18854284709468172No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24110.18117847921294447No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23680.17794717493830464No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT23320.17524189694093176No Hit
GAGTATTAGTAGCAACTACTTAGCCTGGTACCAGCAGAGACCTGGCCAGG22930.17231117911044447No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT22650.1702070740013767No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG21750.1634438790079445No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21160.15901022895669453No Hit
GTAATAAACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA20880.15690612384762673No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20630.15502745857167333No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20460.15374996618402503No Hit
GTTCGGTACCATACTGCTGACAATAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAG20440.15359967296194876No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20220.15194644751910977No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC19450.14616015846917335No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19420.14593471863605895No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19390.14570927880294454No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19340.14533354574775387No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18920.14217738808415217No Hit
GTACCGAACCTCGCACTTTCGGCGGAGGGACCAAGGTGGAGACCAAACGAACTGTGGCTGCACCATCTGTCTTCA18870.1418016550289615No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC18790.14120048214065642No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGTAGCAACTACTTAGCCTGGTACC18690.14044901603027507No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18220.1369171253114827No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17910.1345875803693005No Hit
GTATTAGTAGCAACTACTTAGCCTGGTACCAGCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCTCTGGTGCA17760.1334603812037285No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17730.1332349413706141No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA17710.1330846481485378No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG17630.1324834752602327No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17500.13150656931673693No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17440.13105568965050815No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT17360.13045451676220307No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGTAGCAACTACTTAGCC16940.12729835909860138No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT16690.12541969382264798No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16480.12384161499084714No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTATTGTCAGCAGTA16300.1224889759921607No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC15520.11662754033118614No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG15470.11625180727599548No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT15380.11557548777665223No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGATGCACCAGAGATGAGGAGCC15220.11437314200004207No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC15100.11347138266758446No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCATCCTCCTGCTACTCTGGCTCCCAGATCC14900.11196845044682176No Hit
TCTCTGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCAC14770.110991544503326No Hit
CTCCTGTGTAGCCTCTGGATTCGACTTTTATAATTATGCCATGAGCTGGG14500.10896258600529635No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT14480.10881229278322006No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG14140.10625730800792345No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT14010.1052804020644277No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCATCCTCCTGCTACTCTGGCTCCCAGA13910.10452893595404635No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13740.10325144356639804No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGATGCACCAGAGA13580.10204909778978788No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA150.009115163133.3078270
AATGGTA2700.048.1389370
ATCCCGA306.825077E-445.2758646
CGCGTAT1000.044.50689739
GGTATCA12000.043.8659361
TATACAC7450.043.2991143
TGTTAGA8350.043.10552270
GACCGAT6950.042.5503726
CTTATAC7300.041.869391
ACCGATC7100.041.66708827
GTATCGC1400.041.4420635
CCGATCA7350.041.1937328
ACGAGAC7150.040.8540122
AGACCGA7250.040.7758625
TATCTCG4950.040.70792836
CGATCAG7450.040.656129
AGTATCG1700.040.13025334
CACGAGA7300.040.0100321
ATCAGTA7700.039.8110631
TTATACA7850.039.7951242
Produced by FastQC (version 0.11.5)