FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences936695
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATATACAGCCGTGTCTTCGGGTCTCAAGTCGTTCATCTCCAGATACA39070.41710482067268423No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36680.39158957825119173No Hit
GTATATCACTGTGCCAAAGATAGATGGGGATTTGCTGGGTACTTTGGCCA35450.3784583028627248No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT32540.347391626943669No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24160.25792814096370753No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT24150.2578213826272159No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23890.25504566587843425No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22420.23935219041416894No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22350.2386048820587278No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22060.23550889030047134No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21010.22429926496885327No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT20450.21832079812532362No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18510.19760968084595307No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18510.19760968084595307No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC17430.18607978050486018No Hit
AGCCAGGACACAGCATGGACATGAGGGTCCCCGCCCAGCTCCTGGGGCTC17000.18148917203572135No Hit
GGACTGGAGTGGGTGGCATCTGCTTCTTATGATACAACGACTAAAAATTA16980.18127565536273813No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT16640.1776458719220237No Hit
GTTCATGGCTGGTTGGCCTGGTATCAGCAGAAGCCAGGAAAAGTCCCTAACCTCCTCATCTACAAGACGTCTACT16380.1748701551732421No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15770.16835789664725445No Hit
GGTTAGGGACTTTTCCTGGCTTCTGCTGATACCAGGCCAACCAGCCATGAACTCTCTGACTGGCCCGGCAAGTGA14800.15800233800756916No Hit
CTCCAGTCCCCTGCCTGGAATCTGGCGGACCCAGTGCATGCCGAAACTGG14730.15725502965212795No Hit
GCACTGAACACAGAGGACTCACCATGGAATCTGGGCTGAACTGGCTTTTC14580.15565365460475394No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14550.15533337959527913No Hit
GCATGGACATGAGGGTCCCCGCCCAGCTCCTGGGGCTCCTGCTGCTCTGG14460.15437255456685472No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC14400.15373200454790514No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14230.1519171128275479No Hit
GTCAGAGAGTTCATGGCTGGTTGGCCTGGTATCAGCAGAAGCCAGGAAAAGTCCCTAACCTCCTCATCTACAAGA14210.15170359615456472No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14200.15159683781807312No Hit
GTGTATCTGGAGATGAACGACTTGAGACCCGAAGACACGGCTGTATATCACTGTGCCAAAGATAGATGGGGATT14140.1509562877991235No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13800.14732650435840908No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13710.14636567932998468No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13540.14455078760962747No Hit
CCTTCCACCCTGTCTGCTTCTATAGGAGACACAGTCACCATCACTTGCCGGGCCAGTCAGAGAGTTCATGGCTGG13420.14326968757172825No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13290.14188182919733744No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13240.14134803751487943No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13070.13953314579452222No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12640.1349425373253834No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12640.1349425373253834No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA12620.1347290206524002No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12070.12885731214536217No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG12020.12832352046290416No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT11980.12789648711693774No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11970.12778972878044614No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG11860.12661538707903855No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11820.12618835373307213No Hit
GGACCAAGGTGGATGTCAGCCGAACTGTGGCTGCACCATCTGTCTTCATC11670.12458697868569812No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11600.1238396703302569No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11600.1238396703302569No Hit
CAGCATGGACATGAGGGTCCCCGCCCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACA11500.1227720869653409No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11450.12223829528288291No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11440.1221315369463913No Hit
GAGTGAATTCTGTGTCAGATCCACTGCCGCTGAATCTTGATGGGACCCCATATTCTAAAGTAGACGTCTTGTAGA11350.1211707119179669No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG11310.1207436785720005No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11260.12020988688954248No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC11020.11764768681374407No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGGGGTTCCCTGG11010.11754092847725246No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10910.11647334511233647No Hit
GAATTCACTCTCACCATCAGCAACCTGCAGCCTGATGATTTTGCAATTTATTACTGTCAACAATATAATGATTA10760.11487197006496246No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG10410.11113542828775642No Hit
CTCCTGTGTGGCCTCTGGATTCACTTTCACCAGTTTCGGCATGCACTGGGTCCGCCAGATTCCAGGCAGGGGA10410.11113542828775642No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGGCTGACATCCACCTTGGTCC10380.11081515327828163No Hit
CCAGATACACCGTGTTCATGGAATCGTCCCTGGAGATGATGAAGCGGCCCTTCACGGACTCTGCATAATTTTTAG10360.11060163660529841No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10330.11028136159582361No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA10260.10953405324038241No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10150.1083597115389748No Hit
GTCCCTAACCTCCTCATCTACAAGACGTCTACTTTAGAATATGGGGTCCCATCAAGATTCAGCGGCAGTGGA10130.1081461948659916No Hit
GCCATGAACTCTCTGACTGGCCCGGCAAGTGATGGTGACTGTGTCTCCTATAGAAGCAGACAGGGTGGAAGGAGA10120.10803943652949999No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT9990.1066515781551092No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9970.10643806148212598No Hit
CCCTGAGACTCTCCTGTGTGGCCTCTGGATTCACTTTCACCAGTTTCGGC9970.10643806148212598No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9910.10579751146317638No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT9900.10569075312668477No Hit
CTCTCTGACTGGCCCGGCAAGTGATGGTGACTGTGTCTCCTATAGAAGCAGACAGGGTGGAAGGAGACTGGGTCA9790.10451641142527716No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9730.10387586140632755No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG9660.10312855305088636No Hit
ATCTTGATGGGACCCCATATTCTAAAGTAGACGTCTTGTAGATGAGGAGGTTAGGGACTTTTCCTGGCTTC9610.10259476136842836No Hit
GAGTACGGGAGCTCTGGGAGAGGAGCCCAGCACTAGAAGTCGGCGGTGTTTCCATTCGGTGATCAGCACTGAACA9590.10238124469544516No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9580.10227448635895355No Hit
GACCCAGCCAGGACACAGCATGGACATGAGGGTCCCCGCCCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGG9560.10206096968597037No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC9550.10195421134947875No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9530.10174069467649555No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAATCTGGGCTGAACTGGCTTTTCCTCGTTGCTCTTTTAAGAGG9450.10088662798456276No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9370.10003256129262994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACCG10650.078.8191970
GTTATTA550.00598890454.50806870
CGGGATT252.814195E-454.18174417
GGTCCAA600.008448265549.96573370
ATGCCGT2700.048.4382944
CGTATGC2550.048.38870241
TCGTATG2350.048.08714340
ATCTCGT2150.047.6458137
GTGGTAT1750.046.4514121
CGCTTCA8400.046.3967570
CGAGACA3150.045.197323
GCCGTCT2700.044.70396846
AATGGTA2054.9112714E-1043.8723570
ACGAGAC3250.043.78321522
GACAGGT2100.043.54821
GTATGCC3000.043.48121342
TATACAC3300.043.094513
GTATCAA17950.042.6451571
CCCACGA3350.042.46491219
TTATACA3300.042.068452
Produced by FastQC (version 0.11.5)