FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences936695
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATATACAGCCGTGTCTTCGGGTCTCAAGTCGTTCATCTCCAGATACACCGTGTTCATGGAATCGTCCCTGGA35330.3771772028248256No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA33150.3539038854696566No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32430.34621728524226136No Hit
GTATATCACTGTGCCAAAGATAGATGGGGATTTGCTGGGTACTTTGGCCAGTGGGGCCAGGGAACCCCGGTCACC31800.33949151004329053No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31000.33095084312396245No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26140.27906629158904445No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT22330.23839136538574457No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC22060.23550889030047134No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA21670.23134531517729892No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21550.2300642151393997No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20100.2145842563481176No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA17200.18362433876555334No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17160.18319730541958695No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC17060.18212972205467096No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16690.1781796636044817No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA16230.17326878012586808No Hit
GTTCATGGCTGGTTGGCCTGGTATCAGCAGAAGCCAGGAAAAGTCCCTAA16150.17241471343393527No Hit
GGACTGGAGTGGGTGGCATCTGCTTCTTATGATACAACGACTAAAAATTA15900.16974575502164527No Hit
CTCCAGTCCCCTGCCTGGAATCTGGCGGACCCAGTGCATGCCGAAACTGGTGAAAGTGAATCCAGAGGCCACACA15710.16771734662830484No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15490.16536866322548963No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15250.16280646314969122No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC15220.1624861881402164No Hit
AGCCAGGACACAGCATGGACATGAGGGTCCCCGCCCAGCTCCTGGGGCTC15100.16120508810231718No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA14880.15885640469950196No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC14530.15511986292229596No Hit
GTGTATCTGGAGATGAACGACTTGAGACCCGAAGACACGGCTGTATATCACTGTGCCAAAGATAGATGGGGATTT14410.15383876288439674No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14220.15181035449105632No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13880.1481805710503419No Hit
GGTTAGGGACTTTTCCTGGCTTCTGCTGATACCAGGCCAACCAGCCATGAACTCTCTGACTGGCCCGGCAAGT13820.1475400210313923No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13560.14476430428261067No Hit
GAGTGAATTCTGTGTCAGATCCACTGCCGCTGAATCTTGATGGGACCCCATATTCTAAAGTAGACGTCTTGTA13510.14423051260015265No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG13190.14081424583242144No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13150.14038721248645503No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG13120.14006693747698024No Hit
GTCAGAGAGTTCATGGCTGGTTGGCCTGGTATCAGCAGAAGCCAGGAAAAGTCCCTAACCTCCTCATCTACAA13100.13985342080399704No Hit
CCTTCCACCCTGTCTGCTTCTATAGGAGACACAGTCACCATCACTTGCCG12980.13857232076609782No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT12950.13825204575662303No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12720.1357966040173162No Hit
GCATGGACATGAGGGTCCCCGCCCAGCTCCTGGGGCTCCTGCTGCTCTGG12650.13504929566187499No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12210.13035192885624455No Hit
GCACTGAACACAGAGGACTCACCATGGAATCTGGGCTGAACTGGCTTTTC12160.12981813717378657No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11870.12672214541553012No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA11760.12554780371412252No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG11540.1231991203113073No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11390.1215977452639333No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11260.12020988688954248No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11200.11956933687059289No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11140.11892878685164328No Hit
CTCCTGTGTGGCCTCTGGATTCACTTTCACCAGTTTCGGCATGCACTGGGTCCGCCAGATTCCAGGCAGGG11080.11828823683269368No Hit
GGACCAAGGTGGATGTCAGCCGAACTGTGGCTGCACCATCTGTCTTCATC10970.11711389513128607No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC10870.11604631176637006No Hit
GAATTCACTCTCACCATCAGCAACCTGCAGCCTGATGATTTTGCAATTTATTACTGTCAACAATATAATGATTA10830.11561927842040365No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10640.11359087002706324No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT10600.11316383668109685No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10590.11305707834460524No Hit
GTCCCTAACCTCCTCATCTACAAGACGTCTACTTTAGAATATGGGGTCCCATCAAGATTCAGCGGCAGTG10530.11241652832565563No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10340.1103881199323152No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10220.109107019894416No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG10190.10878674488494121No Hit
CCAGATACACCGTGTTCATGGAATCGTCCCTGGAGATGATGAAGCGGCCC10170.10857322821195799No Hit
GCCATGAACTCTCTGACTGGCCCGGCAAGTGATGGTGACTGTGTCTCCTATAGAAGCAGACAGGGTGGAAGGA10150.1083597115389748No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGGGGTTCCCTGG10130.1081461948659916No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCT10080.1076124031835336No Hit
ATCTTGATGGGACCCCATATTCTAAAGTAGACGTCTTGTAGATGAGGAGGTTAGGGACTTTTCCTGGCTTCT9970.10643806148212598No Hit
CAGCATGGACATGAGGGTCCCCGCCCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT9730.10387586140632755No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9690.10344882806036115No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9590.10238124469544516No Hit
TCATTATATTGTTGACAGTAATAAATTGCAAAATCATCAGGCTGCAGGTT9530.10174069467649555No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9470.10110014465754594No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGGCTGACATCCACCTTGGTCC9380.10013931962912154No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGAGT200.0068130884146.924570
TCGGTTT455.853968E-497.9496870
GATCAGT600.001835125473.4622570
AATGGTA2900.055.7299970
TCTACTT11000.049.42006370
CTCGTAT1801.2041401E-648.9748470
GAGCAAA21550.046.3613370
CGCTTCA9300.044.23533670
TAGGCAA1358.265099E-443.53318870
GTGGTAT2000.042.250711
ACTCAGT10650.037.24846370
TTTTAGT8350.036.95107370
GTATCAA17800.036.838821
GAGCCAT11250.036.56787570
TATAAGA853.6598067E-935.7544442
TTGTCAA1650.002216336335.6180670
CAGCAAA10100.034.91275870
TTAAGAC400.002883875533.7680854
TATCAAC21050.031.4420192
ACTTCCA3751.6368722E-731.34389570
Produced by FastQC (version 0.11.5)