FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences750580
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG26860.357856590903035No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG23250.30976045191718404No Hit
CTATTACTGTTATTCTGCGGCTGACAACAATTGGATGTTCGGCGGAGGCACCAAACTGACCGTCCTAGGTCAGCC20280.2701910522529244No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTGCCTCCGCCGAACATCCAATTGTTGTCAGCCGCAGAATAACAG19750.2631298462522316No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19680.26219723413893253No Hit
GTTCGGGCCTCAGGGATCCCTGAGCGATTCTCCGGCTCCAACTCAGGAAC17340.23102134349436437No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT17160.22862319806016682No Hit
GTGTATGTAGTAGCCGCTATAGGTGTATCCAGAGGCTGTGCAGGAGACCCTCACTGAGGCCCCAGGCTTCATCAC16540.22036291934237523No Hit
GTCTACATGGAGTTGACCAGCCTGAAATCAGACGACTCGGCCGTCTATTA15890.21170294971888406No Hit
CTACATACACTGGGTGCGACGGGCCCCTGGACAAGGCCTTGAGTGGGTGGGCCGCATCAATCCTAGTAGTGGCGG15670.20877188307708705No Hit
GTATTGGTGATTTATAAAGACACTGTTCGGGCCTCAGGGATCCCTGAGCG15650.20850542247328732No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15310.20397559220869194No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTGCCTC15110.20131098617069465No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15100.2011777558687948No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15050.20051160435929546No Hit
GACCTAGGACGGTCAGTTTGGTGCCTCCGCCGAACATCCAATTGTTGTCAGCCGCAGAATAACAGTAATAGTCAC14960.1993125316421967No Hit
CTTATGACCTGACACAGCCATCTTCTGTGTCAGTGTTTCCGGGACAGACAGCCACCATCACCTGCTCAGGAAATA14120.18812118628260813No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14080.18758826507500867No Hit
CAGTAATAGTCACCCTCATCCTCAACTTGGGCCCCGCTGATGGTCAAGGT14050.18718857416930906No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13540.180393828772416No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13520.18012736816861627No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG13510.1799941378667164No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13000.17319939246982333No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12780.1702683258280263No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12470.16613818646913053No Hit
CTGTTATTCTGCGGCTGACAACAATTGGATGTTCGGCGGAGGCACCAAACTGACCGTCCTAGGTCAGCCCAAGG12360.16467265314823204No Hit
GCCATCTTCTGTGTCAGTGTTTCCGGGACAGACAGCCACCATCACCTGCTCAGGAAATATATTAGAAAGAAAATA12020.16014282288363665No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG11780.1569452956380399No Hit
GAATAACAGTAATAGTCACCCTCATCCTCAACTTGGGCCCCGCTGATGGTCAAGGTGCTTGTGGTTCCTGAGT11250.14988408963734712No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11140.1484185563164486No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11110.14801886541074902No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT11110.14801886541074902No Hit
GTCTGGGGCTGAGGTGATGAAGCCTGGGGCCTCAGTGAGGGTCTCCTGCACAGCCTCTGGATACACCTATAGCGG11100.14788563510884917No Hit
GGCCAGGCCCCTGTATTGGTGATTTATAAAGACACTGTTCGGGCCTCAGG10970.14615364118415092No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGC10830.14428841695755282No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10820.14415518665565297No Hit
GTCTCTGTGGCCTCTTATGACCTGACACAGCCATCTTCTGTGTCAGTGTTTCCGGGACAGACAGCCACCATCAC10800.14388872605185324No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10640.1417570412214554No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC10390.13842628367395882No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10390.13842628367395882No Hit
GTGCTAGTGGACCTGTCCCTGGTCAAGGAGATCCTGCCGTGAAATTTCTG10330.1376269018625596No Hit
CCCCTGTATTGGTGATTTATAAAGACACTGTTCGGGCCTCAGGGATCCCT10200.13589490793786138No Hit
CACCTATAGCGGCTACTACATACACTGGGTGCGACGGGCCCCTGGACAAGGCCTTGAGTGGGTGGGCCGCATCAA10080.13429614431506304No Hit
ATACAGGGGCCTGGCCTGACTTCTGCTGGAACCACCGAACATATTTTCTTTCTAATATATTTCCTGAGCAGGTGA10030.1336299928055637No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC10020.13349676250366385No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG9830.13096538676756642No Hit
CAATTGGATGTTCGGCGGAGGCACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGT9790.13043246555996696No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAG9570.12750139891816994No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9350.12457033227637293No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9270.12350448986117402No Hit
GTTTGGTGCCTCCGCCGAACATCCAATTGTTGTCAGCCGCAGAATAACAGTAATAGTCACCCTCATCCTCAACT9170.12217218684217539No Hit
GGATGAGGGTGACTATTACTGTTATTCTGCGGCTGACAACAATTGGATGTTCGGCGGAGGCACCAAACTGACCG9160.12203895654027551No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT9090.12110634442697647No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8990.11977404140797784No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA8640.1151109808414826No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT8620.11484452023768285No Hit
ATAAAGACACTGTTCGGGCCTCAGGGATCCCTGAGCGATTCTCCGGCTCCAACTCAGGAACCACAAGCACCTTGA8570.11417836872818353No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC8550.11391190812438381No Hit
CCATGTAGACTGTGCTAGTGGACCTGTCCCTGGTCAAGGAGATCCTGCCGTGAAATTTCTGAGCGACGTTTGAGC8460.11271283540728502No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA8350.11124730208638652No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTGCCTCCGCCGAACATCCAATTGTTG8270.11018145967118761No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT8220.1095153081616883No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8210.10938207785978842No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTGCCTCCGCCGAACATCCAAT8190.1091156172559887No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT8140.10844946574648938No Hit
GTCCTCTAGAGAATCCCCTGAGAGCTCCGTTCCTCACCATGGACTGGACCTGGAGGATCCTCTTCTTGCTTACAG8110.10804977484078979No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8070.10751685363319034No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAAGTCAAAGTCATCGGACG8070.10751685363319034No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACT8060.10738362333129046No Hit
GGGTTCCCTGGCCCCAGAAGTCAAAGTCATCGGACGTACTCCCAGAATTA8060.10738362333129046No Hit
CCACAAGCACCTTGACCATCAGCGGGGCCCAAGTTGAGGATGAGGGTGAC8050.1072503930293906No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8000.10658424151989128No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC7940.1057848597084921No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC7790.10378640517999414No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG7740.10312025367049482No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG7690.1024541021609955No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG7630.10165472034959631No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCT7610.1013882597457966No Hit
CTACACAGTCTCTGTGGCCTCTTATGACCTGACACAGCCATCTTCTGTGTCAGTGTTTCCGGGACAGACAGCCAC7590.10112179914199684No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG7530.10032241733059769No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG7520.10018918702869781No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1800.0112.69421470
AATGGTA2950.057.9053270
TAATCCA200.00783716553.38146670
CGTTGTT200.00706038252.29316755
TACTCCG905.407446E-447.45019570
TCTTATA652.4920155E-1047.0130352
GGTATCA4800.046.6991
CGCGTAT451.983164E-645.5754839
CTACTAG4750.042.70517770
TACGCTG2200.041.7039929
TATACAC2450.041.5761533
CTACGCT2250.040.76907728
TTATACA2500.040.744632
CGTATGC1100.040.4942441
CCCACGA2450.040.18224719
TGGTATC1100.040.127292
GGGAGTA9350.039.96473770
CTTATAC2550.039.9563641
CGAGACT2400.039.6049223
GTATCAA12900.039.4917561
Produced by FastQC (version 0.11.5)