FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences750580
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT27480.36611686962082657No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24680.3288123850888646No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21590.2876442218018066No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19980.2661941431959285No Hit
CTATTACTGTTATTCTGCGGCTGACAACAATTGGATGTTCGGCGGAGGCACCAAACTGACCGTCCTAGGTCAGCC18520.24674251911854833No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTGCCTCCGCCGAACATCCA17930.23888193130645632No Hit
GTCTACATGGAGTTGACCAGCCTGAAATCAGACGACTCGGCCGTCTATTA15620.20810573156758772No Hit
GTGTATGTAGTAGCCGCTATAGGTGTATCCAGAGGCTGTGCAGGAGACCCTCACTGAGGCCCCAGGCTTCATCAC15260.20330944069919263No Hit
GTTCGGGCCTCAGGGATCCCTGAGCGATTCTCCGGCTCCAACTCAGGAACCACAAGCACCTTGACCATCAGCGGG15120.2014442164725945No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15120.2014442164725945No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA15120.2014442164725945No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14530.19358362866050255No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG14340.19105225292440514No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14160.18865410749020756No Hit
CTACATACACTGGGTGCGACGGGCCCCTGGACAAGGCCTTGAGTGGGTGGGCCGCATCAATCCTAGTAGTGGCG14120.18812118628260813No Hit
GACCTAGGACGGTCAGTTTGGTGCCTCCGCCGAACATCCAATTGTTGTCAGCCGCAGAATAACAGTAATAGTCAC13710.1826587439047137No Hit
GTATTGGTGATTTATAAAGACACTGTTCGGGCCTCAGGGATCCCTGAGCG13500.17986090756481654No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTA13480.1795944469610168No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTGCCTC13340.1777292227344187No Hit
CAGTAATAGTCACCCTCATCCTCAACTTGGGCCCCGCTGATGGTCAAGGTGCTTGTGGTTCCTGAGTTGGAGCCG13270.17679661062111968No Hit
CTTATGACCTGACACAGCCATCTTCTGTGTCAGTGTTTCCGGGACAGACA12580.16760371979002903No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG12480.1662714167710304No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12450.1658717258653308No Hit
GCCATCTTCTGTGTCAGTGTTTCCGGGACAGACAGCCACCATCACCTGCT12230.1629406592235338No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA12190.16240773801593436No Hit
CTGTTATTCTGCGGCTGACAACAATTGGATGTTCGGCGGAGGCACCAAACTGACCGTCCTAGGTCAGCCCAAG12040.16040928348743638No Hit
GTGCTAGTGGACCTGTCCCTGGTCAAGGAGATCCTGCCGTGAAATTTCTGAGCGACGTTTGAGCCGCCACTACTA11460.15268192597724428No Hit
GTCTCTGTGGCCTCTTATGACCTGACACAGCCATCTTCTGTGTCAGTGTTTCCGGGACAGACAGCCACCATCAC11110.14801886541074902No Hit
GTCTGGGGCTGAGGTGATGAAGCCTGGGGCCTCAGTGAGGGTCTCCTGCACAGCCTCTGGATACACCTATAGCG10950.1458871805803512No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG10850.14455487756135255No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG10820.14415518665565297No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG10710.14268965333475445No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10430.13895920488155827No Hit
CCCCTGTATTGGTGATTTATAAAGACACTGTTCGGGCCTCAGGGATCCCTGAGCGATTCTCCGGCTCCAACTCAG10270.13682752005116045No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10170.1354952170321618No Hit
GAATAACAGTAATAGTCACCCTCATCCTCAACTTGGGCCCCGCTGATGGTCAAGGTGCTTGTGGTTCCTGAGTTG10070.13416291401316316No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10000.1332303018998641No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCT10000.1332303018998641No Hit
GGCCAGGCCCCTGTATTGGTGATTTATAAAGACACTGTTCGGGCCTCAGG9940.13243092008846494No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC9880.13163153827706575No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTT9790.13043246555996696No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9780.1302992352580671No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9780.1302992352580671No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTG9580.12763462922006982No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC9230.12297156865357457No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT9150.12190572623837566No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG9080.1209731141250766No Hit
ATACAGGGGCCTGGCCTGACTTCTGCTGGAACCACCGAACATATTTTCTTTCTAATATATTTCCTGAGCAGGT9060.12070665352127688No Hit
ATAAAGACACTGTTCGGGCCTCAGGGATCCCTGAGCGATTCTCCGGCTCCAACTCAGGAACCACAAGCACCTT9050.120573423219377No Hit
CACCTATAGCGGCTACTACATACACTGGGTGCGACGGGCCCCTGGACAAG9020.12017373231367744No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACG9010.12004050201177756No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC8950.11924112020037837No Hit
GTTTGGTGCCTCCGCCGAACATCCAATTGTTGTCAGCCGCAGAATAACAGTAATAGTCACCCTCATCCTCAACTT8920.11884142929467878No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8900.11857496869087905No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8890.11844173838897919No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGG8730.11631005355858136No Hit
GGATGAGGGTGACTATTACTGTTATTCTGCGGCTGACAACAATTGGATGTTCGGCGGAGGCACCAAACTGACCGT8610.114711289935783No Hit
GTCCTCTAGAGAATCCCCTGAGAGCTCCGTTCCTCACCATGGACTGGACCTGGAGGATCCTCTTCTTGCTTACA8600.11457805963388312No Hit
GGGTTCCCTGGCCCCAGAAGTCAAAGTCATCGGACGTACTCCCAGAATTAGGACTCGCACAATAATAGACGGCC8550.11391190812438381No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGG8540.11377867782248394No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG8360.1113805323882864No Hit
CAATTGGATGTTCGGCGGAGGCACCAAACTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTT8250.10991499906738789No Hit
ATAATAGACGGCCGAGTCGTCTGATTTCAGGCTGGTCAACTCCATGTAGACTGTGCTAGTGGACCTGTCCCTGGT8030.10698393242559087No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGTTTGGTGCCTCCGCCGAACATCCAATTGTTGT8020.10685070212369101No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG7980.10631778091609156No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA7870.10485224759519306No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT7830.10431932638759359No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC7800.10391963548189401No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC7800.10391963548189401No Hit
CCATGTAGACTGTGCTAGTGGACCTGTCCCTGGTCAAGGAGATCCTGCCGTGAAATTTCTGAGCGACGTTTGAGC7720.10285379306669509No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGCAA200.007177968144.3754970
GTAGTTT1002.0489263E-457.750270
GGGAGTA8800.052.50018370
AAAATAT11150.050.49905470
TAACAGT11850.047.51598770
GCGGTAT306.8555766E-445.2323231
GTGGTAT1650.045.2323231
GAGCCAT8700.044.8061970
ACCAGGT1307.477348E-444.4232370
AATGGTA3107.6397555E-1041.91546670
GGTAGAA13100.041.87991370
GGTATCA6250.041.794671
TGGTATC1750.040.6602672
CCATAGA350.001463719338.7705651
TCTAAGC350.001474308538.713743
GTATCAA14850.035.637591
CGGTGGT7850.034.94438670
AACGATT501.9749692E-434.15209239
AAAACAT1700.00279439333.97070370
TACAAAA1700.00279439333.97070370
Produced by FastQC (version 0.11.5)