FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1390792
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT59340.4266633687855553No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC56780.4082565904894478No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGG41450.29803162514596No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT41010.2948679601263165No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40220.2891877433865021No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG37480.2694867384914495No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC36280.2608585611651491No Hit
GATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTAGATCTGGGACAGATTTCA35870.2579106005786631No Hit
GACTTACAGTACCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCT34370.24712537892078756No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA34070.24496833458921247No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG33670.24209227548044565No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC32530.23389550702046027No Hit
CACCATCACCGGTCTGCAACCGGAGGATTGTGGATTTTACTTCTGTCAACAGACTTACAGTACCTGGACGTTCGG32280.23209797007748106No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG30780.22131274841960552No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30680.22059373364241383No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30030.21592013759066778No Hit
GTACCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC29690.21347548734821598No Hit
GCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGTTTAAATGATTGCTAATGCTCTGACT29640.21311597995962014No Hit
GTCTCCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAA29580.21268457109330513No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT28550.2052787188882306No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG28510.20499111297735392No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC27090.19478110314123176No Hit
CTGTAAGTCTGTTGACAGAAGTAAAATCCACAATCCTCCGGTTGCAGACCGGTGATGGTGAGAGTGAAATCTGTC26980.1939901868863209No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC26450.19017940856720486No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26330.18931659083457483No Hit
GTGATGGTGAGAGTGAAATCTGTCCCAGATCTACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAATTGGA25810.185577713993178No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGCGACATCCAGATGACCCAGTC25410.18270165488441117No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24850.17867517213213766No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC24820.17845946769898013No Hit
GATTTCACTCTCACCATCACCGGTCTGCAACCGGAGGATTGTGGATTTTACTTCTGTCAACAGACTTACAGTAC23620.16983129037267974No Hit
ATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAATCATTTAAACTGGTATCAGCAGAAA23400.168249457862858No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23080.16594861057584456No Hit
CTCTTATACCGACTACAACCCGTCCTTCCAAGGCCACGTCACCATCTCAACTGACAAGTCCATCAATACTGCCTA22780.16379156624426944No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGCGACA22690.16314445294479693No Hit
GATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGTTTAAAT22420.16120311304637933No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT21860.1571766302941058No Hit
CATTTAAACTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATGCTGCATCCAATTTGCAAAG21450.15422866970761984No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21020.15113690616569553No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20990.150921201732538No Hit
GAGTGAAATCTGTCCCAGATCTACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAATTGGATGCAGCATAGA20940.15056169434394218No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20480.14725422636886035No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20450.14703852193570283No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGTTTAAATGATTGCTAATGCTCTGACTTGCCCGGCAAGTGA20420.1468228175025453No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19830.1425806303171143No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19490.1401359800746625No Hit
GTATAAGAGTAACTAGGATCAATCCTCCCCATCCACTCCAGGCCTTTCCCGGGCATCTGGCGCACCCAGTTGATC19450.13984837416378582No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA19120.1374756253990532No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17920.12884744807275278No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT17380.1249647682759176No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17320.12453335940960258No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGCGACATCCAGATGACCCAGTCTCCATCCTCC17310.12446145793188343No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT17050.122592019511185No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17030.12244821655574664No Hit
GTCCAGGTACTGTAAGTCTGTTGACAGAAGTAAAATCCACAATCCTCCGG16940.12180110325627413No Hit
TCCAGGTACTGTAAGTCTGTTGACAGAAGTAAAATCCACAATCCTCCGGT16470.11842173380347312No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT16340.11748701459312393No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG16310.11727131015996642No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16260.11691180277137055No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16120.11590518208330217No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTAGATCTGGGACAGATTTCACT16110.11583328060558301No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15890.11425144809576125No Hit
GTTTAAATGATTGCTAATGCTCTGACTTGCCCGGCAAGTGATGGTGAC15870.11410764514032293No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15830.11382003922944624No Hit
GTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAATCATTTAAACTGGTA15700.11288532001909703No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15620.11231010819734367No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15410.1108001771652411No Hit
GTCTGCAACCGGAGGATTGTGGATTTTACTTCTGTCAACAGACTTACAGT15140.10885883726682352No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15120.10871503431138516No Hit
CCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAATCAT14960.10756461066787845No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14950.10749270919015927No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT14940.10742080771244011No Hit
CTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGC14930.10734890623472093No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14680.10555136929174169No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC14570.1047604530368308No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG14540.10454474860367331No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14480.10411333973735828No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGG14380.10339432496016658No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14370.1033224234824474No Hit
CATCAAGGTTCAGTGGCAGTAGATCTGGGACAGATTTCACTCTCACCATCACCGGTCTGCAACCGGAGGATTG14360.10325052200472824No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT14300.10281911313841322No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14300.10281911313841322No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14230.10231580279437902No Hit
ACCTTGATGGGACCCCACTTTGCAAATTGGATGCAGCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTC14220.10224390131665986No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14200.10210009836122151No Hit
GTCCCAGATCTACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAATT14160.10181249245034485No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14150.10174059097262567No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14060.10109347767315313No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14060.10109347767315313No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13970.1004463643736806No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTA200.00795441850.74208527
AATGGTA4300.048.17777670
TGTTAGA15600.043.00116770
TATTCTA2002.1693268E-839.45989670
TCGTATG3250.038.9861840
CGTATGC3350.037.87639241
GTATCAA25850.037.687421
GTGGTAT2850.036.667051
ATGCCGT3600.035.43279644
TATACAC3750.035.0483443
CACGAGA3850.035.04231621
TCTTCGG3102.3646862E-1135.00474570
TATCTCG3350.034.58135636
TCTCCGA3800.034.57972311
ATCTCGT3400.034.1112537
ACTCCTA3650.033.46007531
TCCTATC3500.033.00689333
GACGGAC3700.032.9007226
ACGAGAC3900.032.8681322
CCGTCTT3900.032.86800447
Produced by FastQC (version 0.11.5)