FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1390792
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT57660.4145839205287347No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA56050.4030077826159483No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG40820.2935018320496523No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT40170.28882823599790625No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39420.2834356251689685No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC37390.26883962519197696No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37230.2676892015484702No Hit
GATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTAGATCTGGGACAGATTTCA35850.25776679762322474No Hit
GACTTACAGTACCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT33990.24439312276745914No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA33650.24194847252500734No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC33430.24036664001518557No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG32340.23252937894379608No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32010.23015663017906343No Hit
GTCTCCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTT29590.21275647257102429No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29530.21232506370470927No Hit
GTACCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC29480.21196555631611344No Hit
CACCATCACCGGTCTGCAACCGGAGGATTGTGGATTTTACTTCTGTCAACAGACTTACAGTACCTGGACGTTCG29260.21038372380629164No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA29020.2086580883410316No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC28730.20657294548717567No Hit
GCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGTTTAAATGATTGCTAATGCTCTGACTT27470.19751335929456024No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG26520.19068271891123909No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC25420.18277355636213036No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24800.17831566474354182No Hit
CTGTAAGTCTGTTGACAGAAGTAAAATCCACAATCCTCCGGTTGCAGACCGGTGATGGTGAGAGTGAAATCTGTC24620.17702143814459675No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT24610.17694953666687757No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24560.1765900292782817No Hit
GTGATGGTGAGAGTGAAATCTGTCCCAGATCTACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAATTGGAT24430.17565531006793253No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGCGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTATA24400.175439605634775No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA23730.17062220662759062No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC23730.17062220662759062No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23460.16868086672917304No Hit
ATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAATCATTTAAACTGGTATCAGCAGAAA22660.1629287485116394No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGCGACAT22370.1608436056577835No Hit
GATTTCACTCTCACCATCACCGGTCTGCAACCGGAGGATTGTGGATTTTACTTCTGTCAACAGACTTACAGTAC22210.15969318201427676No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA22000.1581832509821742No Hit
CTCTTATACCGACTACAACCCGTCCTTCCAAGGCCACGTCACCATCTCAA21540.15487578300709237No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGC21500.15458817709621567No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21120.15185592094288722No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21000.1509931032102572No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTAGATCTGGGACAGATTTCACTCTCACCATCACCGGTCTGCAACCG20530.14761373375745618No Hit
GAGTGAAATCTGTCCCAGATCTACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAATTGGATGCAGCATA20260.1456723938590386No Hit
GATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGTTTAAATGATTGCTAATGCTCTGACTTGCCCG20180.14509718203728522No Hit
CATTTAAACTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATGCTGCATCCAATTTGCAAAGT19360.13920126086431328No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGTTTAAATGATTGCTAATGCTCTGACTTGCCCGGCAAGT18910.13596569436695063No Hit
GTATAAGAGTAACTAGGATCAATCCTCCCCATCCACTCCAGGCCTTTCCCGGGCATCTGGCGCACCCAGTT18740.13474336924572475No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18330.13179540865923878No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17830.12820033477328024No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGCGACATCCAGATGACCCAGTCTCCATCCTCC17820.1281284332955611No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17720.12740941851836937No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT17440.1253961771422326No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17080.12280772394434251No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCA17010.1223044136003083No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG16870.12129779291223994No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC16660.11978786188013735No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16280.1170556057268089No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA16210.1165522953827747No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16190.11640849242733636No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15870.11410764514032293No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA15860.11403574366260374No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15510.11151919194243282No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15500.11144729046471363No Hit
GTCCAGGTACTGTAAGTCTGTTGACAGAAGTAAAATCCACAATCCTCCGGTTGCAGACCGGTGATGGTGAGAGT15470.11123158603155613No Hit
GTTTAAATGATTGCTAATGCTCTGACTTGCCCGGCAAGTGATGGTGACT15460.11115968455383694No Hit
TCCAGGTACTGTAAGTCTGTTGACAGAAGTAAAATCCACAATCCTCCGGT15440.1110158815983986No Hit
GTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA15390.11065637420980276No Hit
GTCTGCAACCGGAGGATTGTGGATTTTACTTCTGTCAACAGACTTACAGT15260.10972165499945354No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGCGACATCCAGAT15240.10957785204401521No Hit
CCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCG15210.10936214761085769No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15190.10921834465541937No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14920.10727700475700176No Hit
CTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGC14390.10346622643788575No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14340.1031067190492899No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGAT14340.1031067190492899No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT14230.10231580279437902No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14080.10123728062859148No Hit
GCCCCTAACCTCCTGATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTA14050.10102157619543398No Hit
CTCCTGATCTATGCTGCATCCAATTTGCAAAGTGGGGTCCCATCAAGGTT14050.10102157619543398No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCTAT9900.068.3585570
AATGGTA4050.060.43995770
CGGCAAA2007.6215656E-1057.59572270
GTTATTA800.006216882353.99598770
CTACGAA200.0064594253.4851961
GAGCAAA32900.043.32808770
TATTCTA2351.7622006E-742.89042770
GTAGTTT1859.149643E-538.91602370
GTATCAA29900.038.4871521
ATATCAT8800.037.63356870
GAGCCAT13450.036.39878570
AAGGACA34500.035.89298670
GAGGGTT4052.746674E-1035.55291470
AGGTACG502.1680252E-433.6166652
ATCAACG35050.032.6984563
TTGGTTT4855.2750693E-1132.65736870
TCAACGC34900.032.6487354
TATCAAC35200.032.470642
GTGGTAT3750.032.3021971
AACGCAG35800.032.015736
Produced by FastQC (version 0.11.5)