FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1222959
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCACCCTCATCTTCAGGCTGGAGTCCACTGATGGCCAGGGAGG63910.5225849762747566No Hit
GATTATTACTGTGCAGCTTGGGATGCCGACCTGAATGGTCCCTTCTTCGG54560.4461310640830969No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAG50490.4128511258349626No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA47790.39077352552293243No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC45840.37482859196424406No Hit
GTCCTGGGCCCAGTCTGTTCTGTCTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGACAGTCACGATTTCTTG45060.36845061854076877No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAG41820.34195749816633264No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG39730.3248678001470205No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC39720.3247860312569759No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTTCTGTCTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGACAGTCACGA34990.286109346265901No Hit
GTTCTGTCTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGACAGTCAC32880.2688561104664997No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC29940.24481605679340027No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT29680.2426900656522418No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACTGAGGACGGTGACCTTGGTC29290.23950107894050413No Hit
CATCTATACTTATAATCAGCGACCCTCAGGGGTCCCTGACCGATTTTCTGGATCTACGTCTGGCACCTCAGCCTC28480.2328777988468951No Hit
GTATAGATGAGGAGTTTGGGGGCCGTTCCTGGAAGATGCTGGAACCAGTT27980.22878935434466732No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT27000.22077600312030085No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC26550.21709640306829583No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTTCTGTCTCAGCCAC26270.21480687414704827No Hit
GCCTTGGGCTGACTGAGGACGGTGACCTTGGTCCCAGGTCCGAAGAAGGGACCATTCAGGTCGGCATCCCAAG25880.21161788743531057No Hit
CTTATAATCAGCGACCCTCAGGGGTCCCTGACCGATTTTCTGGATCTACGTCTGGCACCTCAGCCTCCCTGGCCA25580.20916482073397388No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG25270.20662998514259268No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG25140.20556698957201344No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG23590.1928928116151073No Hit
CCTCAGGGGTCCCTGACCGATTTTCTGGATCTACGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGACTCCAGC23200.18970382490336962No Hit
CCCCTGAGGGTCGCTGATTATAAGTATAGATGAGGAGTTTGGGGGCCGTTCCTGGAAGATGCTGGAACCAGTTGA22650.18520653595091904No Hit
AGTCTGTTCTGTCTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGACAGTCACGATTTCTTGTTCTGGGAACA21950.17948271364780014No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAG21330.17441304246503767No Hit
GTATCAACGCAGAGTACGGGAACTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG21260.17384066023472577No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC20930.17114228686325544No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGACAGTCACGATTTCTTGTTCTGGGAACATCTCCAACGTCGGACGTTT20780.1699157535125871No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC20700.16926160239223065No Hit
GATTATAAGTATAGATGAGGAGTTTGGGGGCCGTTCCTGGAAGATGCTGGAACCAGTTGACAGCAAAACGTCCGA20560.16811683793160687No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA20230.16541846456013654No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACT20130.16460077565969095No Hit
GTCTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGACAGTCACGATTTCTTGTTCTGGGAACATCTCCAACG19930.16296539785879985No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGC19640.16059410004750774No Hit
GAATGGTCCCTTCTTCGGACCTGGGACCAAGGTCACCGTCCTCAGTCAGCCCAAGGCCAACCCCACTGTCAC19590.16018525559728494No Hit
GACTGAGGACGGTGACCTTGGTCCCAGGTCCGAAGAAGGGACCATTCAGG19310.15789572667603738No Hit
GTACGGGAACTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC19140.15650565554527995No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC18540.15159952214260658No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA18110.1480834598706907No Hit
TCAGTGGACTCCAGCCTGAAGATGAGGGTGATTATTACTGTGCAGCTTGGGATGCCGACCTGAATGGTCCCTTCT17320.14162371755717076No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA17250.14105133532685887No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG17160.14031541531645786No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC16960.13868003751556673No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT16870.13794411750516575No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA16490.13483689968347262No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG16420.13426451745316073No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG16200.1324656018721805No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG16100.13164791297173495No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTT15430.1261693973387497No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT15430.1261693973387497No Hit
GTTTGGGGGCCGTTCCTGGAAGATGCTGGAACCAGTTGACAGCAAAACGT15410.1260058595586606No Hit
GATGAGGAGTTTGGGGGCCGTTCCTGGAAGATGCTGGAACCAGTTGACAGCAAAACGTCCGACGTTGGAGATGT15220.12445225064781404No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG14840.1213450328261209No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14610.11946434835509612No Hit
GGACCATTCAGGTCGGCATCCCAAGCTGCACAGTAATAATCACCCTCATC14430.11799250833429412No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGG14430.11799250833429412No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCG14350.11733835721393768No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGT14150.11570297941304655No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAG14100.11529413496282379No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGT14090.11521236607277922No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA13960.11414937050219999No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC13810.11292283715153165No Hit
TCCTCAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG13720.11218691714113066No Hit
GTGGACTCCAGCCTGAAGATGAGGGTGATTATTACTGTGCAGCTTGGGAT13710.1121051482510861No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC13490.11030623267010586No Hit
CTCCTCATCTATACTTATAATCAGCGACCCTCAGGGGTCCCTGACCGATT13450.10997915710992764No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13280.10858908597917019No Hit
GCTTGGGATGCCGACCTGAATGGTCCCTTCTTCGGACCTGGGACCAAGGT13170.10768962818868007No Hit
GGCCATCAGTGGACTCCAGCCTGAAGATGAGGGTGATTATTACTGTGCAGCTTGGGATGCCGACCTGAATGGTCC13130.10736255262850185No Hit
GAGTACGGGAACTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC13100.10711724595836819No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA12930.10572717482761074No Hit
TTATTACTGTGCAGCTTGGGATGCCGACCTGAATGGTCCCTTCTTCGGACCTGGGACCAAGGTCACCGTCCTCAG12870.10523656148734341No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT12710.1039282592466305No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC12330.10082104142493738No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTTCTGTCTCAGCCACCCTCAGCGT12330.10082104142493738No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)