FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258934
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATATTGCTTTGGCAATGCATCTGCAGAGCAGGTGA67140.5333083386420575No Hit
CCCCTATACTGGTGATATATAAAGACAGTAAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTC54490.43282650242189025No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG37770.3000157275917562No Hit
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGG33400.26530382053388024No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG33370.2650655236890893No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC33340.26482722684429844No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATAAGTATCACTGCTGTCTGCTGAT31740.2521180617887832No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATAAGTATCA29250.23233942367113766No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGCAGATGCATTGCCAAAG29220.23210112682634673No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC26660.2117664627375224No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGCAGA26470.21025724938717993No Hit
GTCTTTATATATCACCAGTATAGGGGCCTGGCCTGGCTTCTGCTGGTACC26430.2099395202607921No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG26190.20803314550246477No Hit
GTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGA26190.20803314550246477No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT25790.204855854238586No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGG24980.1984218394292314No Hit
GACTATTACTGTCAATCAGCAGACAGCAGTGATACTTATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTA24270.1927821474358465No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24000.19063747583272833No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC23760.18873110107440103No Hit
ATATAAAGACAGTAAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCTGGGACAACAGTCACGT23670.1880162105400283No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23290.18499778383934343No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAACGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG22370.1776900139324222No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22230.17657796199006462No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21970.1745127226685434No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG21740.17268578019181308No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21550.17117656684147065No Hit
GTGTATTACTGTGCGAGGGGTCCCGACTTTGGAGTGGCGAGGCAATACTA20560.1633127709633706No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20350.16164469304983423No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20230.1606915056706706No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT20100.15965888600990996No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19960.15854683406755238No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19800.15727591756200088No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGCAGATGCATTGCCAAAGCAATATGCTTATTGG19590.1556078396484645No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGC19560.1553695428036736No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19520.1550518136772857No Hit
CTATTACTGTCAATCAGCAGACAGCAGTGATACTTATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTACG19480.15473408455089782No Hit
CAGTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTA19110.15179509013180992No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCA18690.1484589343047372No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG18300.14536107532245535No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18230.14480504935127655No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAG17870.14194548721378564No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTG17850.1417866226505917No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17510.1390859250762947No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC17370.1379738731339371No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG17050.13543204012283408No Hit
CAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGT16600.13185758745097043No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16540.1313809937613886No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16530.13130156147979163No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16250.12907745759507647No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16200.12868029618709162No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGC16040.1274093796815401No Hit
GTATTACTGTGCGAGGGGTCCCGACTTTGGAGTGGCGAGGCAATACTACTACATGGACGTCTGGGGCAAAGGGAC16030.1273299473999431No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT15770.1252647080784219No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15740.125026411233631No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15650.12431152069925826No Hit
GCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTA15630.12415265613606433No Hit
GTAAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCTGGGACAACAGTCACGTTGACCATCAG15490.12304060419370674No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT15400.122325713659334No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATAAGTA15240.12105479715378248No Hit
GTCCTGGGGACACTGACACCGAGGGTGGCTGTGTCAGCTCATAGGAGGCC15100.11994274521142491No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG14820.11771864132670974No Hit
ACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATAAGTATCAC14680.11660658938435216No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAGGAATCATCCCTATCTTTGGT14650.11636829253956124No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC14470.11493851147081578No Hit
GCAATATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCCCTATACTGGTGATATATAAAGACAGTAAGAGGCC14380.11422362093644306No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14210.11287327214929456No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14040.11152292336214606No Hit
GCATATTGCTTTGGCAATGCATCTGCAGAGCAGGTGATCCTGGCCGTCTGTCCTGGGGACACTGACACCGAGGG13910.11049030370138546No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG13640.10834563209826728No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13600.10802790297187938No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA13510.10731301243750666No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC13490.1071541478743127No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13440.10675698646632786No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13210.10493004398959756No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG13210.10493004398959756No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12970.10302366923127028No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12940.10278537238647936No Hit
CAGTAAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCTGGGACAACAGTCACGTTGACCATCAG12880.10230877869689754No Hit
CCAGTATAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATATTGCTTTGGCAATGCATCTGCAGAGCAGG12830.10191161728891268No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCT12710.10095842990974904No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG12670.10064070078336115No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG12650.10048183622016722No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)