FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258934
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATATTGCTTTGGCAATGCATCTGCAGAGCAGGT64750.5143240233403816No Hit
CCCCTATACTGGTGATATATAAAGACAGTAAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCTG54420.4322704764507115No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG38470.3055759873035441No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT31940.2537067074207226No Hit
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGG31890.25330954601273775No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC30000.2382968447909104No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC29310.23281601736071947No Hit
GTCTTTATATATCACCAGTATAGGGGCCTGGCCTGGCTTCTGCTGGTACC27790.22074231055798002No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC26800.21287851467987995No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGCAGATGCATTGCCAAAG26790.21279908239828302No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT25330.20120196928512538No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATAAGTATCA25330.20120196928512538No Hit
GTGTATTACTGTGCGAGGGGTCCCGACTTTGGAGTGGCGAGGCAATACTACTACATGGACGTCTGGGGCAAAGG25090.1992955945267981No Hit
ATATAAAGACAGTAAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCTGGGACAACAGTCACGTT24980.1984218394292314No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGCAGAT24950.1981835425844405No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACG24400.1938147670966071No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC23740.1885722365112071No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23310.18515664840253737No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23020.18285311223622525No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22490.17864320131158584No Hit
GACTATTACTGTCAATCAGCAGACAGCAGTGATACTTATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTA22310.17721342024284037No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA22120.17570420689249794No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21670.17212975422063428No Hit
CTATTACTGTCAATCAGCAGACAGCAGTGATACTTATGTGGTATTCGGCG20940.16633119766405546No Hit
GTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGACTGTTGTCCCAGAGCTGGAGCCAGA20770.16498084887690698No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATA20630.1638687969345494No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAACGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCA20590.1635510678081615No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20040.15918229232032816No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGG19950.15846740178595542No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGCAGATGC19920.15822910494116452No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19850.1576730789699857No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG19700.15648159474603116No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT18260.14504334619606746No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG18190.1444873202248887No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTG17990.14289867459294928No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCA17880.1420249194953826No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17830.14162775808739775No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT17780.1412305966794129No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGG17760.14107173211621896No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17470.13876819594990683No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA17370.1379738731339371No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16900.1342405558988795No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC16440.13058667094541893No Hit
GTATTACTGTGCGAGGGGTCCCGACTTTGGAGTGGCGAGGCAATACTACTACATGGACGTCTGGGGCAAAGGGAC16410.130348374100628No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG16400.130268941819031No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCT16370.1300306449742401No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG16310.1295540512846583No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAGCTACAGGTGTCCAGTCCCAGGTGCA16070.12764767652633102No Hit
CAGTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGGCTGTGCTCGTGGATTCGTCCGCG16030.1273299473999431No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT15520.12327890103849765No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG15500.1231200364753037No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTT15210.12081650030899156No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14790.11748034448191882No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14640.11628886025796428No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14570.11573283428678548No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATAAGTAT14570.11573283428678548No Hit
GCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGGGGTCCCGACTTTGGA14440.11470021462602488No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC14430.11462078234442791No Hit
CAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGACTGTTGTCCCAGAGCTGGAGCCA14390.11430305321804002No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14150.11239667845971274No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14140.11231724617811578No Hit
GCAATATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCCCTATACTGGTGATATATAAAGACAGTAAGAGGCC14090.11192008477013092No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAG14080.11184065248853396No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAGGAATCATCCCTATCTTTGGT13970.11096689739096728No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACG13930.1106491682645794No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT13910.11049030370138546No Hit
GTAAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAGCTCTGGGACAACAGTCACGTTGACCATCAGTG13830.1098548454486097No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC13810.10969598088541575No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG13710.10890165806944606No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13470.10699528331111878No Hit
GTCCTGGGGACACTGACACCGAGGGTGGCTGTGTCAGCTCATAGGAGGCCTCAGAGCCTGTGCAGAGAGTGAGGA13450.10683641874792484No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG13320.10580379908716422No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGC13200.10485061170800057No Hit
ACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATAAGTATCACTGCTGTCTGCTGATTGACAGTAATA13120.10421515345522482No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC13060.103738559765643No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAATT702.6634638E-587.2060370
AATGGTA3600.084.78364670
CGCGGTA14700.063.32819770
ACTTTCA1001.5590424E-461.04422870
CTGTTTA1051.9843654E-458.13735670
TGTCTTT1706.253904E-753.8625570
AAAGTCA2104.6002242E-850.87018670
TGGTATC2800.045.6551632
GTCTTTA3700.045.4892461
GGTATCA10650.044.5666471
AGCAGAA22300.042.42984470
CTTTATA4050.040.6929473
TCTTTAT4200.040.048392
GCTGATT23400.039.78309670
AAACTCA1958.854289E-539.13091370
GGTAGAA30300.038.27855770
AAGCGGT9650.037.9549670
TATAGGG16050.037.9374282
AGTTCCA13300.037.8657870
TTATATA4400.037.461965
Produced by FastQC (version 0.11.5)