FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences742621
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA37520.5052375303149251No Hit
GTATTACTGTCAGGAGTATATTATCCCACCGTTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTG29510.3973763198185885No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT24960.33610684319457707No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24020.32344897329862743No Hit
GTATTAACACCAACTACTTCGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGGTCCTCATCTATGGTGCA22200.29894118264902286No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21900.29490143693754955No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA19520.2628527876265282No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18870.2541000052516694No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18080.24346200821145644No Hit
GTGTTAATACTCTGACTGGCCCTGCAGGAGAGGCTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGGAGA17380.23403593488468544No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT17180.2313427710770366No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16310.2196275085137641No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA15760.2122213080427297No Hit
GTGTATTACTGTCAGGAGTATATTATCCCACCGTTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA15470.2083162205216389No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15330.2064310058562847No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA15220.2049497657620778No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTGAGTTCTTGGCGTTGTCTC14750.19862083081410303No Hit
ATATTATCCCACCGTTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCT14630.1970049325295137No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14210.19134928853345112No Hit
CCATAGATGAGGACCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCGAAGTAGTTGGTGTTAATACTCTGA13710.18461637901432898No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13680.18421240444318165No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT13360.1799033423509435No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA13310.17923005139903128No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTTCATCCAATACGTAC13300.17909539320864884No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC13230.17815278587597172No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGATGCACCATAGATGAGGACCC12170.16387901769543278No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12100.1629364103627557No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11890.1601085883647244No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCAGCCTCTCCTGCAGGGCCAGTCAGAGTATTAACACCAACTACTTCGCC11790.15876200646089997No Hit
CTCCTGTGTAGGCTCTGGATTCACGATTAGTACGTATTGGATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG11670.15714610817631067No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGATGCACCATAGA11470.1544529443686618No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC11450.15418362798789692No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11270.15175978056101294No Hit
GTGTATTACTGTGCGAGATATAGTGGCTACGATTCCCCGTACTGGGGCCA11110.14960524951489387No Hit
GGGCTGGAGTGGGTGGCCAACATAAAGCAAGATGGAAGTGAGAAAAATTA10790.1452961874226557No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10790.1452961874226557No Hit
GTATATTATCCCACCGTTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA10460.14085246714003508No Hit
GGAGTATATTATCCCACCGTTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA10260.13815930333238624No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGGAGTA10230.13775532876123892No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9990.13452353219206029No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC9800.13196502657479386No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCAGCCTCTCCTGC9650.12994515371905724No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9480.1276559644825557No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9470.12752130629217326No Hit
GAATTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAG9170.12348156058069998No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT9060.1220003204864931No Hit
ATATACTCCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCT8910.11998044763075646No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8900.11984578944037402No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCAGCCTCTCCTGCAGGGCCAGTCAGAGTATTAACAC8720.11742194201349006No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC8710.1172872838231076No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8690.11701796744234272No Hit
ACATAAAGCAAGATGGAAGTGAGAAAAATTATGTGGACACCGTGAAGGGCCGATTCACCATCTCCAGAGACAACG8680.11688330925196029No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8650.11647933468081297No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAACACCAACTACTTCGCCTGGTACC8610.11594070191928318No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC8600.11580604372890073No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG8540.11499809458660609No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC8480.11419014544431143No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8360.11257424715972213No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGG8270.11136232344628014No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA8240.1109583488751328No Hit
GAGTATTAACACCAACTACTTCGCCTGGTACCAGCAGAAACCTGGCCAGG8110.10920779240016106No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8080.10880381782901372No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8050.10839984325786639No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG8050.10839984325786639No Hit
GTGTAGGCTCTGGATTCACGATTAGTACGTATTGGATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAG7860.1058413376406No Hit
GCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGGTTTTC7850.10570667945021754No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7810.10516804668868776No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7790.10489873030792288No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7720.10395612297524577No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7700.10368680659448089No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT7580.10207090830989159No Hit
CTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGGTTT7530.10139761735797938No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGGAGTATATTATCCCA7430.10005103545415493No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAG150.008356943137.2249870
TCGTGCA358.8749506E-488.2160670
GTACGAG150.002627126467.1245351
GTCAGAA500.003653601761.7512470
CGTTTCG200.00537315856.03019366
GAGTCTT200.007081071452.2543749
TCTGGAG12850.052.0600670
ATCGGAA200.00811348550.48620627
TATCGGA200.00813733950.44872726
AACGGAG854.8880326E-448.43234370
GGAAACG1507.2283E-848.0287470
TGTTAGA11000.044.90999270
ATAAGAG607.1668182E-944.7406463
CGGCAAA958.455743E-443.334270
TCGTATG1750.043.03009840
GTATAAG551.8239007E-742.715611
GTATCAA13000.042.3400881
GGTATCA4650.041.140841
CGTATGC1850.040.7685541
GTGATTT350.001542937438.35687319
Produced by FastQC (version 0.11.5)