FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences742621
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTGAGTTCTTGGCGTTGTCTCTGGA33150.44639190111779764No Hit
GTATTACTGTCAGGAGTATATTATCCCACCGTTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGT29240.3937405486782625No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23650.31846662025447703No Hit
GTATTAACACCAACTACTTCGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGGTCCTCATCTATGGTGCAT21730.29261224770104804No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA21540.29005374208378165No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA20360.2741640756186534No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19620.2641993695303526No Hit
GTGTTAATACTCTGACTGGCCCTGCAGGAGAGGCTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGGA18740.2523494487766977No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18260.24588585563834042No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA18230.24548188106719307No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT16560.22299396327332516No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16340.2200314830849114No Hit
GTGTATTACTGTCAGGAGTATATTATCCCACCGTTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA15970.21504913004076104No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC15700.21141335890043508No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA15050.2026605765255763No Hit
ATATTATCCCACCGTTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA15020.20225660195442896No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTTCATCCAATACGTACTAATCGTGAATCCAGAGCCTACACA14840.199832754527545No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC14830.19969809633716257No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA14730.19835151443333815No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14410.19404245234109996No Hit
CCATAGATGAGGACCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCGAAGTAGTTGGTGTTAATACTCT14020.1887907829161847No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTGAGTTCTTGGCGTTGTCTCTG12910.17384372378373356No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12870.1733050910222038No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA12170.16387901769543278No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC12050.16226311941084348No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGATGCACCATAGATGAGGACCCTG11910.1603779047454893No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA11690.15741542455707555No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11630.15660747541478087No Hit
GGGCTGGAGTGGGTGGCCAACATAAAGCAAGATGGAAGTGAGAAAAATTA11620.15647281722439846No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11530.15526089351095645No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCAGCCTCTCCTGCAGGGCCAGTCAGAGTATTAACACCAACTACTTCGCCTG11370.1531063624648374No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11300.1521637551321603No Hit
GTGTATTACTGTGCGAGATATAGTGGCTACGATTCCCCGTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCA11170.15041319865718852No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT11120.14973990770527631No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA11050.1487973003725992No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGATGCACCATA10720.14435358008997862No Hit
CTCCTGTGTAGGCTCTGGATTCACGATTAGTACGTATTGGATGAACTGGGTCCGCCAGGCTCCAGGGAAGGG10470.14098712533041755No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT10430.14044849256888778No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA10360.13950588523621066No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10180.1370820378093267No Hit
GTATATTATCCCACCGTTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT9860.13277297571708854No Hit
ATATACTCCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCA9840.13250365933632366No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCAGCCTCTCCTGC9700.13061844467096945No Hit
GGAGTATATTATCCCACCGTTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT9560.12873323000561523No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9270.1248281424845244No Hit
ACATAAAGCAAGATGGAAGTGAGAAAAATTATGTGGACACCGTGAAGGGCCGATTCACCATCTCCAGAGACAAC9070.12213497867687555No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8600.11580604372890073No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCAGCCTCTCCTGCAGGGCCAGTCAGAGTATTAACAC8430.11351685449239922No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8400.1131128799212519No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8390.11297822173086944No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8160.10988108335207326No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC7950.10705326135404197No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7900.10637997040212975No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC7900.10637997040212975No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG7900.10637997040212975No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA7870.10597599583098243No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC7720.10395612297524577No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGG7630.1027441992618038No Hit
GAATTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT7620.10260954107142135No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC7510.10112830097721448No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG7510.10112830097721448No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7490.1008589845964496No Hit
GGTTTCTGCTGGTACCAGGCGAAGTAGTTGGTGTTAATACTCTGACTGGCCCTGCAGGAGAGGCTGGCTCTTTCC7450.10032035183491983No Hit
GTGTAGGCTCTGGATTCACGATTAGTACGTATTGGATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGT7440.10018569364453739No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAACACCAACTACTTCGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAG7430.10005103545415493No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAA106.759041E-4317.9337870
TCGGTTT559.4973034E-486.7092170
ATGGCAA959.870862E-566.93342670
TTGTAAT150.002658761666.922163
TATGAAT750.00325113563.58675870
TGAGCTT1001.2723367E-463.58675470
GCTTGTA453.8187864E-852.106711
GCTGGCA950.00828565150.20006670
CACTCGT950.00828565150.20006670
CGGGATC307.2970026E-444.6628917
GAGCCAT6200.041.02371670
GTATCAA13250.036.9100881
GGTATCA5900.036.903661
TTGGTTT1750.002008073736.3352970
ACCAGGT2659.988735E-635.99250470
AATGGTA1800.00230578335.32597770
AAGGACA16300.035.10925370
GTGTATA400.002999608633.497171
ACGTACC400.0030115233.47009313
GAATACG400.003016494233.4588249
Produced by FastQC (version 0.11.5)