FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708478
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCACTATGCTAGCTCACCTCTGTTCACTTTCGGCCCTGGGACCAAAATGGATATCAAACGAA37890.5348084203038062No Hit
GTAATATACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCCTCAGGGAGAACTGGTTCTTGGACGTGTCTACGGA37520.5295859575032676No Hit
GTATATTACTGTGCGAGACACGAGGGTGGGAGCTACCCATATAGGGCTTTTGATATCTGGGGCCAAGGGACAA25410.3586561615180711No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT25040.35343369871753255No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22980.3243572842064256No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA21590.30473776179359136No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC18320.2585824824482906No Hit
GTGTATTACTGTCAGCACTATGCTAGCTCACCTCTGTTCACTTTCGGCCC18240.25745330130222815No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT16540.23345820194840208No Hit
CATCTATGGTGCGTCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA14980.2114391696001852No Hit
ATGCTAGCTCACCTCTGTTCACTTTCGGCCCTGGGACCAAAATGGATATCAAACGAACTGTGGCTGCACCA14500.20466408272381076No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14390.20311145864797495No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13640.19252538540363992No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13590.1918196471873509No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGTTAAGTAGTTGCCGCTAACACTCTGA13180.1860325938137811No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC13170.1858914461705233No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12980.18320964094862507No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12810.18081013101324248No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12610.17798717814808646No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12520.17671684935876628No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA12500.17643455407225064No Hit
GTCTCTGGTGGCTCCATCAACAATAATAATTACTACTGGGACTGGATCCGCCAGCCCCCAGGGAAGGAGCTGGAG12420.17530537292618825No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC11900.16796569547678264No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11730.1655661855414No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCGGCAACTACTTAACCTGGTACC11710.1652838902548844No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCACTA11500.1623197897464706No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11450.1616140515301816No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11450.1616140515301816No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11250.1587910986650256No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCGGCAACTACTTAACC11240.1586499510217678No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10620.14989879713978416No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10240.14453518669598775No Hit
TCTATGGTGCGTCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCAC10220.14425289140947214No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT9970.14072420032802713No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT9970.14072420032802713No Hit
CTACTTAACCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCGTCCAGCAGGGCCA9730.1373366568898399No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCATTTTGGTCC9710.13705436160332432No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCC9600.1355017375274885No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGACGCACCATAGATGAGGAGCC9550.13479599931119948No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT9190.12971468415391868No Hit
GTCAGAGTGTTAGCGGCAACTACTTAACCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTA9110.12858550300785626No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG8800.12420992606686446No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGACGCACCATAGA8690.12265730199102866No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC8660.12223385906125525No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAG8640.12195156377473966No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8540.12054008734216164No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA8530.12039893969890386No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8470.11955205383935705No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCTGGCTGTGCCCAGCCA8370.11814057740677902No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCGG8360.11799942976352125No Hit
GTGCAGCCACAGTTCGTTTGATATCCATTTTGGTCCCAGGGCCGAAAGTGAACAGAGGTGAGCTAGCATAG8290.11701139626071663No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCACTATGCTAGCTCA8240.11630565804442763No Hit
GTCTCAGATGCCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACCCT8230.11616451040116983No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8150.11503532925510743No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8000.11291811460624043No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7780.10981286645456881No Hit
CTATTATAGTGGGGTCACCTACTACAACCCGTCCCTCAAGAGGCGAGTTACCATATCCGTAGACACGTCCAAGAA7680.1084013900219908No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC7540.10642532301638159No Hit
CAGTAATATACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCCTCAGGGA7490.1057195848000926No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGTCTCAGAT7260.10247318900516318No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)