FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708478
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCACTATGCTAGCTCACCTCTGTTCACTTTCGGCCCTGGGACCAAAATGGATATCAAACGAA35390.4995215094893561No Hit
GTAATATACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCCTCAGGGAGAACTGGTTCTTGGACGTGTCTACG34140.48187805408213097No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25380.3582327185882977No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21950.30981907695087213No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA21410.30219710421495094No Hit
GTATATTACTGTGCGAGACACGAGGGTGGGAGCTACCCATATAGGGCTTTTGATATCTGGGGCCAAGGGACAATG21360.30149136599866194No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA19810.27961348129370284No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGTCTCA17730.25025477149608033No Hit
GTGTATTACTGTCAGCACTATGCTAGCTCACCTCTGTTCACTTTCGGCCC16730.2361400071703003No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT15540.219343437622622No Hit
CATCTATGGTGCGTCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA14880.21002769316760717No Hit
ATGCTAGCTCACCTCTGTTCACTTTCGGCCCTGGGACCAAAATGGATATCAAACGAACTGTGGCTGCACCAT14060.1984535864204675No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13650.1926665330468977No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGTTAAGTAGTTGCCGCTAACACTCT13140.18546800324074988No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13030.18391537916491407No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC12970.18306849330536729No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC12930.18250390273233608No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC12390.17488192999641486No Hit
GTCTCTGGTGGCTCCATCAACAATAATAATTACTACTGGGACTGGATCCG11820.16683651433072022No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11580.16344897089253302No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCGGCAACTACTTAACCTG11160.15752076987570537No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT11150.15737962223244759No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA11110.15681503165941638No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA11030.15568585051335398No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA10950.15455666936729157No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCGGCAACTACTTAACCTGGTACCAACAGAAACCTGGCCAGGCTCCCAG10920.15413322643751817No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT10920.15413322643751817No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10030.14157108618757394No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9590.13536058988423072No Hit
TCTATGGTGCGTCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT9570.13507829459771512No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9490.1339491134516527No Hit
CTACTTAACCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCGTCCAGCAGGGCCA9140.12900894593762968No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAG9120.12872665065111408No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9100.1284443553645985No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGTCTGGGCACTGTGTGGCC8970.12660943600224708No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA8900.12562140249944245No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC8890.12548025485618466No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8840.12477451663989567No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCATTTTGGTCCCAGGGCCGAAAGTGAACAGAGGTGA8770.12378648313709106No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC8770.12378648313709106No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGACGCACCATAGATGAGGAGCCTG8620.12166926848822404No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCC8570.12096353027193506No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGACGCACCATA8530.12039893969890386No Hit
GTGCAGCCACAGTTCGTTTGATATCCATTTTGGTCCCAGGGCCGAAAGTGAACAGAGGTGAGCTAGCATAGT8280.11687024861745884No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8000.11291811460624043No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCACTATGCTAGCTCA7880.1112243428871468No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCG7830.11051860467085782No Hit
GTCAGAGTGTTAGCGGCAACTACTTAACCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATG7800.1100951617410844No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7750.10938942352479541No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGT7670.10826024237873301No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTTCAGGGCCAGCTCCTCCGACGGCGGC7280.10275548429167877No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)