FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences826938
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGTCAGCAGTATGCTATCTCCCCGCTCACTTTCGGCGGAGGGACCAAGGTTGAGATCAAAGGAACTG70510.852663682162387No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT42610.515274422024384No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36520.44162923943512095No Hit
CAATAATACACTGCAAAATCTTCAGGCTCCAGGGTGCTGATGGCTAGAGTGAAGTCTGTCCCAGACCCACTGCCA36080.43630840522505915No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA30260.36592827999197036No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT24320.2940970181561375No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23590.28526927048944417No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC22450.27148347276337526No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC21890.26471150195056947No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTACTTAGCCTGGTACC20450.24729786271764026No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20300.24548394196421014No Hit
GTATGCTATCTCCCCGCTCACTTTCGGCGGAGGGACCAAGGTTGAGATCAAAGGAACTGTGGCTGCACCA19780.23919568335231903No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19720.23847011505094698No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19620.23726083454866023No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19510.2359306259961448No Hit
GTCTGGGACAGACTTCACTCTAGCCATCAGCACCCTGGAGCCTGAAGATTTTGCAGTGTATTATTGTCAGCAGTA19160.2316981442381412No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19120.2312144320372265No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC18750.22674009417876553No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18400.2225076124207619No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18100.21887977091390162No Hit
GTCCCAGACCCACTGCCACTGAACCTCTCTGGGGTGCCAGTGGCCCTGCTGGATGCACCGTAGATGAGGAGCC17780.21501007330658406No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG17620.21307522450292526No Hit
GTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTACTTAGCC17530.2119868720508672No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGC17200.2079962463933209No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC16030.19384766451656593No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT15990.19336395231565123No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATCTCAACCTTGGTCC15280.1847780607494153No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA15050.18199671559415578No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGAGAACTGGTTCTTGGACGCGTCTAATGA14700.17776423383615217No Hit
GTGTATTATTGTCAGCAGTATGCTATCTCCCCGCTCACTTTCGGCGGAGGGACCAAGGTTGAGATCAAAGGAA14530.1757084569822647No Hit
GTCAGAGTGTTAGCAGCAACTACTTAGCCTGGTACCAGCAGAAACCTGGC14290.1728061837767765No Hit
CCCCAGAGAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTAGCCATCAGCACCCTGGAGCCTGAAGATT14200.17171783132471843No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGCGGGGAGATAGCATACTGCTGACAATAATACACTGCAAAATCTTCAGG14200.17171783132471843No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT13930.1684527739685442No Hit
GTGTTAGCAGCAACTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTACGGTGCA13810.1670016373658001No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13310.16095523485436636No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12990.15708553724704874No Hit
GTGCAGCCACAGTTCCTTTGATCTCAACCTTGGTCCCTCCGCCGAAAGTG12910.15611811284521937No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12840.15527161649361862No Hit
GACTTCACTCTAGCCATCAGCACCCTGGAGCCTGAAGATTTTGCAGTGTATTATTGTCAGCAGTATGCTATCTCC12800.15478790429270395No Hit
GATAGCATACTGCTGACAATAATACACTGCAAAATCTTCAGGCTCCAGGGTGCTGATGGCTAGAGTGAAG12800.15478790429270395No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC12540.15164377498675838No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12260.14825778958035546No Hit
CATCTACGGTGCATCCAGCAGGGCCACTGGCACCCCAGAGAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA12170.14716943712829741No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA12160.14704850907806874No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11990.14499273222418127No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG11670.14112303461686365No Hit
GACCAAGGTTGAGATCAAAGGAACTGTGGCTGCACCATCTGTCTTCATCT11460.13858354556206148No Hit
CCTCATCTACGGTGCATCCAGCAGGGCCACTGGCACCCCAGAGAGGTTCAGTGGCAGTGGGTCTGGGACAGACT11380.13761612116023209No Hit
ATAATACACTGCAAAATCTTCAGGCTCCAGGGTGCTGATGGCTAGAGTGAAGTCTGTCCCAGACCCACTGCCA11170.1350766321054299No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11170.1350766321054299No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTAGCCATCAGCACCCTGGAGCCTGAAGATTTTGCAGTGTA11090.13410920770360052No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC10870.13144879059856968No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10660.12890930154376748No Hit
CCGTAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGTTGCTGCTAACACTCTGA10650.12878837349353883No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTCTCTGGGGTGCCAGTGGCCCTGCTGGATGCACCGTAGA10610.1283046612926241No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10590.12806280519216678No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10490.12685352468988No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10450.12636981248896534No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10250.12395125148439183No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT10200.12334661123324844No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9920.11996062582684554No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9660.1168164965209No Hit
TCTCTGGGGTGCCAGTGGCCCTGCTGGATGCACCGTAGATGAGGAGCCTG9660.1168164965209No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9600.11609092821952795No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT9520.11512350381769855No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG9480.11463979161678384No Hit
CTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTACGGTGCATCCAGCAGGGCCA9440.11415607941586915No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9380.1134305111144971No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9220.1114956623108383No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTACGGTGCATCCAGCAGGGCCACTGGCACCCCAGAGAGGT9190.11113287816015226No Hit
GAGTGTTAGCAGCAACTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG9180.1110119501099236No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTACTTA9140.1105282379090089No Hit
GCAGTATGCTATCTCCCCGCTCACTTTCGGCGGAGGGACCAAGGTTGAGATCAAAGGAACTGTGGCTGCACCA9080.10980266960763684No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9050.10943988545695081No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC8970.10847246105512143No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACC8940.10810967690443539No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG8790.10629575615100527No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8780.10617482810077661No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8740.1056911158998619No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8700.10520740369894721No Hit
CCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCAC8610.10411905124688914No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGT8590.10387719514643176No Hit
CTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGC8520.10303069879483105No Hit
ATGCTATCTCCCCGCTCACTTTCGGCGGAGGGACCAAGGTTGAGATCAAAGGAACTGTGGCTGCACCATCTGTCT8500.10278884269437372No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT8470.10242605854368769No Hit
GGTTTCTGCTGGTACCAGGCTAAGTAGTTGCTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTCTCC8460.102305130493459No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC8360.10109584999117224No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8310.10049120974002887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTAGA10000.053.3487670
CCGATTC200.006694379353.0022359
AAAACCG1301.5206188E-647.3509770
ACAGACG2650.046.45755470
AGCTGCG4250.045.8654570
GGTATCA6650.045.1706921
GCGTAGA21500.041.5146970
GTATCAA17850.038.7618061
CTCTTAT1550.037.0173721
GCTTGAT502.1170432E-433.7511331
AAAGGGG3350.033.68750870
GAGCAAA14250.033.1179970
TATCAAC20750.033.00922
ATCAACG20900.032.933733
TCTTATA1650.032.7184752
TCAACGC21150.032.5444454
CAACGCA21600.031.8664345
TCTAATG3950.031.78003368
ACCGGTA2601.5405385E-731.56731270
AACGCAG21700.031.5640966
Produced by FastQC (version 0.11.5)