FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences826938
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGTCAGCAGTATGCTATCTCCCCGCTCACTTTCGGCGGAGGGACCAAGGTTGAGATCAAAGGAACTGT65300.7896601679932475No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39440.47694023010189396No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA36570.4422338796862642No Hit
CAATAATACACTGCAAAATCTTCAGGCTCCAGGGTGCTGATGGCTAGAGTGAAGTCTGTCCCAGACCCACTGCCA32690.39531379619753837No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29780.360123733580994No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA28640.34633793585492506No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT24070.2910738169004206No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23810.287929687594475No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC20760.2510466322747292No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC20130.24342816511032264No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19920.2408886760555205No Hit
GTCTGGGACAGACTTCACTCTAGCCATCAGCACCCTGGAGCCTGAAGATT19590.2368980503979742No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC19210.2323027844892846No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19080.23073071983631177No Hit
GTATGCTATCTCCCCGCTCACTTTCGGCGGAGGGACCAAGGTTGAGATCAAAGGAACTGTGGCTGCACCAT18930.22891679908288165No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA18890.22843308688196695No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAG18860.22807030273128093No Hit
GTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTACTTAGCCTG17760.21476821720612668No Hit
GTCCCAGACCCACTGCCACTGAACCTCTCTGGGGTGCCAGTGGCCCTGCTGGATGCACCGTAGATGAGGAGCCTG17690.213921720854526No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17070.20642418174034813No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGC17040.2060613975896621No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCA16850.20376376463531728No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA16820.20340098048463126No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT16510.19965221092754235No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGAGAACTGGTTCTTGGACGCGTCTAAT15700.18985703885901964No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15410.1863501254023881No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCA15310.18514084490010133No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14890.180061866790497No Hit
GTGTATTATTGTCAGCAGTATGCTATCTCCCCGCTCACTTTCGGCGGAGGGACCAAGGTTGAGATCAAAGGAA14630.17691773748455145No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATCTCAACCTTGGTCC14600.17655495333386542No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA14440.1746201045302066No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC14420.17437824842974928No Hit
CCCCAGAGAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTAGCC14330.17328989597769118No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13370.1616808031557384No Hit
GTGTTAGCAGCAACTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTACGGTGCAT13260.16035059460322298No Hit
GACTTCACTCTAGCCATCAGCACCCTGGAGCCTGAAGATTTTGCAGTGTATTATTGTCAGCAGTATGCTATCTCC12980.15696460919682007No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA12750.15418326404156055No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGCGGGGAGATAGCATACTGCTGACAATAATACACTGCAAAATCTTCAG12660.15309491158950247No Hit
GTGCAGCCACAGTTCCTTTGATCTCAACCTTGGTCCCTCCGCCGAAAGTG12450.1505554225347003No Hit
GTCAGAGTGTTAGCAGCAACTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTACG11930.14426716392280922No Hit
GATAGCATACTGCTGACAATAATACACTGCAAAATCTTCAGGCTCCAGGGTGCTGATGGCTAGAGTGAAGT11860.14342066757120847No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGGTGAGGAAGATGCTGGCAAAGGATGG11590.14015561021503425No Hit
CATCTACGGTGCATCCAGCAGGGCCACTGGCACCCCAGAGAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA11430.13822076141137546No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC11370.1374951931100034No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT11320.13689055285886004No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11290.136527768708174No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCA11260.136164984557488No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11190.13531848820588727No Hit
GACCAAGGTTGAGATCAAAGGAACTGTGGCTGCACCATCTGTCTTCATCT11030.13338363940222844No Hit
CCTCATCTACGGTGCATCCAGCAGGGCCACTGGCACCCCAGAGAGGTTCAGTGGCAGTGGGTCTGGGACAGACTT10940.13229528695017037No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10930.13217435889994172No Hit
ATAATACACTGCAAAATCTTCAGGCTCCAGGGTGCTGATGGCTAGAGTGAAGTCTGTCCCAGACCCACTGCCA10870.13144879059856968No Hit
TCTCTGGGGTGCCAGTGGCCCTGCTGGATGCACCGTAGATGAGGAGCCTG10790.13048136619674028No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10550.12757909299125206No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10520.12721630884056603No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTCTCTGGGGTGCCAGTGGCCCTGCTGGATGCACCGTA10340.1250396039364499No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG10330.12491867588622123No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10000.12092805022867494No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10000.12092805022867494No Hit
CCGTAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGTTGCTGCTAACACTCT9990.12080712217844627No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTAGCCATCAGCACCC9910.11983969777661686No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9750.11790484897295807No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA9660.1168164965209No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCA9410.11379329526518313No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9240.11173751841129566No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9130.11040730985878024No Hit
GAGTGTTAGCAGCAACTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG9130.11040730985878024No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGAT8960.10835153300489275No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8930.10798874885420673No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT8910.10774689275374937No Hit
GGTTTCTGCTGGTACCAGGCTAAGTAGTTGCTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTCTCC8720.10544925979940456No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTACGGTGCATCCAGCAGGGCCACTGGCACCCCAGAGAGGTT8630.10436090734734647No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC8590.10387719514643176No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACC8580.1037562670962031No Hit
ATGCTATCTCCCCGCTCACTTTCGGCGGAGGGACCAAGGTTGAGATCAAA8510.10290977074460238No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8490.10266791464414503No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA8420.10182141829254429No Hit
CTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTACGGTGCATCCAGCAGGGCCA8380.1013377060916296No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAG8370.10121677804140093No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCTT352.1444507E-4126.0690870
CCGTTAA650.002509725367.88335470
GCGGCAT700.003366563763.0345470
GGAATAA750.004424476558.83223370
AATGGTA3100.056.9344270
AACGTAA200.00711149352.19832651
GTCTCTT4950.050.51252770
GTGAACG250.00846692249.94394368
TATTCTA900.00910026349.0268670
TAAGTCA2002.4681704E-644.12417670
GAGCAAA18450.041.45360670
GTATCAA17750.041.450611
ACTTCCA4800.039.83432470
AAACACA15700.039.34639770
TTGCGCG350.001503123838.56200818
GAACTGT22250.038.1846369
TATCAAC20150.036.645572
ATCAACG20800.035.5003973
TCAACGC21000.035.3228534
CGTTTAT400.002445225134.9231253
Produced by FastQC (version 0.11.5)