FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1175750
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTGGCCAGGGGACCAAGGTCGAGATCAAACGAACTGTGGCTGCACCA64020.5445035083988944No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT56300.47884329151605354No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49340.4196470338082075No Hit
ACTTTAGACAATGGGGTCCCTACAAGATTCAACGGCAGTGGCTCTGGGACAGAGTTCACTCTCACCATCAGCAGC44920.382054008079949No Hit
GGCCAAAACTGTGCACATACCCATCGTATTCTTGGCAGTAGTAAGTGGCAAAATCATCAGGCTGCAGGCTGCTGA43410.36921114182436743No Hit
GTAATAGACAGCCGTGTCCGCGGCGGTCACAGAGGTCAGCCTCAGGGAGG42630.3625770784605571No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCCACTTACTACTGCCAAGAATACGATGGGTA36810.31307675951520303No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT36330.3089942589836275No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC33190.2822879013395705No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32860.2794811822241123No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32520.2765894110142462No Hit
GTCTAAAGTAGACGCCTTATGGATCAGGAGTTTAGGGGCTTTTCCTGGCT31920.27148628534977676No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30890.2627259196257708No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC29460.2505634701254518No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28170.23959174994684246No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28070.23874122900276418No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG27640.23508398894322774No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27060.2301509674675739No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26560.22589836274718267No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25730.2188390389113332No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC25710.21866893472251756No Hit
GTCTATTACTGTGCGAGTCGCCTCTTTACAACTGATCTGCCGTATGACTA25530.2171379970231767No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25170.214076121624495No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACA23990.20403997448437167No Hit
GAATACGATGGGTATGTGCACAGTTTTGGCCAGGGGACCAAGGTCGAGATCAAACGAACTGTGGCTGCACCA23490.19978736976398043No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT22370.19026153519030406No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCGACCTTGGTCC21800.18541356580905805No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATG21620.1838826281097172No Hit
GAGTGAACTCTGTCCCAGAGCCACTGCCGTTGAATCTTGTAGGGACCCCATTGTCTAAAGTAGACGCCTTATGGA20480.17418668934722517No Hit
CTACAAGATTCAACGGCAGTGGCTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTT20370.1732511163087391No Hit
GTGCAGCCACAGTTCGTTTGATCTCGACCTTGGTCCCCTGGCCAAAACTG20280.17248564745906866No Hit
GTCTCTGGTGACTCCATCACTTATCCCACTTTTTACTGGGCCTGGATCCGCCAATCCCCCGGGAAGGGGCTGGAG20270.17240059536466085No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAATCTCCTTCCACCT20270.17240059536466085No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGG19510.16593663618966617No Hit
GTGTTGATATGTGGTTGGCCTGGTATCAGCACAAGCCAGGAAAAGCCCCTAAACTCCTGATCCATAAGGCGTCTA19150.16287476079098448No Hit
GTATCAGCACAAGCCAGGAAAAGCCCCTAAACTCCTGATCCATAAGGCGT19090.16236444822453752No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18770.15964278120348713No Hit
ATCGTATTCTTGGCAGTAGTAAGTGGCAAAATCATCAGGCTGCAGGCTGC18750.1594726770146715No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18440.15683606208802892No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18110.1540293429725707No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC18010.15317882202849245No Hit
AGCCAGGAAAAGCCCCTAAACTCCTGATCCATAAGGCGTCTACTTTAGAC17740.1508824154794812No Hit
CTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCCACTTACTACTGCCAAGAATA17710.15062725919625772No Hit
ATCTTGTAGGGACCCCATTGTCTAAAGTAGACGCCTTATGGATCAGGAGTTTAGGGGCTTTTCCTGGCTTG17550.1492664256857325No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17140.14577928981501168No Hit
GAGTGTTGATATGTGGTTGGCCTGGTATCAGCACAAGCCAGGAAAAGCCCCTAAACTCCTGATCCATAAGGCG17090.14535402934297256No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16970.14433340421007868No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16860.1433978311715926No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA16620.1413565809058048No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16540.1406761641505422No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16460.13999574739527962No Hit
GTATGTGCACAGTTTTGGCCAGGGGACCAAGGTCGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT16370.13923027854560918No Hit
GTGCACATACCCATCGTATTCTTGGCAGTAGTAAGTGGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAG16310.13871996597916222No Hit
GTCCCAGAGCCACTGCCGTTGAATCTTGTAGGGACCCCATTGTCTAAAGT16180.13761428875186052No Hit
GTTGATATGTGGTTGGCCTGGTATCAGCACAAGCCAGGAAAAGCCCCTAAACTCCTGATCCATAAGGCGTCTACT16070.13667871571337442No Hit
TCCATAAGGCGTCTACTTTAGACAATGGGGTCCCTACAAGATTCAACGGCAGTGGCTCTGGGACAGAGTTCAC16040.13642355943015097No Hit
GTGGGGGACAGAGTCTCCATCTCTTGCCGGGCCAGTCAGAGTGTTGATATGTGGTTGGCCTGGTATCAGCACAAG15740.13387199659791624No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15690.1334467361258771No Hit
TCCCAGGACCAGTCCTCCCTGAGGCTGACCTCTGTGACCGCCGCGGACACGGCTGTCTATTACTGTGCGAGTCGC15340.13046991282160322No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15320.1302998086327876No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15240.129619391877525No Hit
GTAGTAAGTGGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGAGCCACTGCCGT14460.12298532851371465No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT14410.12256006804167553No Hit
CTCCTGATCCATAAGGCGTCTACTTTAGACAATGGGGTCCCTACAAGATTCAACGGCAGTGGCTCTGGGACAGAG14380.12230491175845207No Hit
GTCAGAGTGTTGATATGTGGTTGGCCTGGTATCAGCACAAGCCAGGAAAAGCCCCTAAACTCCTGATCCATAAGG14360.1221348075696364No Hit
CCTTCCACCTTGTCTGCGTCTGTGGGGGACAGAGTCTCCATCTCTTGCCGGGCCAGTCAGAGTGTTGATATGTGG14170.12051881777588773No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT14070.11966829683180949No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13920.11839251541569211No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT13920.11839251541569211No Hit
GTCTCCATCTCTTGCCGGGCCAGTCAGAGTGTTGATATGTGGTTGGCCTGGTATCAGCACAAGCCAGGAAAAGCC13730.11677652562194345No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13600.11567084839464173No Hit
CACCTGCACTGTCTCTGGTGACTCCATCACTTATCCCACTTTTTACTGGG13450.11439506697852435No Hit
CCCAATCTCCTTCCACCTTGTCTGCGTCTGTGGGGGACAGAGTCTCCATC13300.11311928556240697No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG13220.11243886880714438No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13220.11243886880714438No Hit
GTCCTGGGACGTGTCAACGGAAATGGCGACTCGACTCTTGAGGGACAAGT13090.11133319157984266No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13060.1110780352966192No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG13020.11073782691898787No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12990.11048267063576439No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12860.10937699340846267No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12740.10835636827556877No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGA12660.10767595152030619No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT12490.10623006591537315No Hit
CCTTATGGATCAGGAGTTTAGGGGCTTTTCCTGGCTTGTGCTGATACCAGGCCAACCACATATCAACACTCTGA12380.1052944928768871No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12380.1052944928768871No Hit
GTCTGCGTCTGTGGGGGACAGAGTCTCCATCTCTTGCCGGGCCAGTCAGAGTGTTGATATGTGGTTGGCCTGGTA12330.10486923240484797No Hit
TTCCTGGCTTGTGCTGATACCAGGCCAACCACATATCAACACTCTGACTGGCCCGGCAAGAGATGGAGAC12290.10452902402721667No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12280.10444397193280885No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12270.10435891983840101No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCGACCT12270.10435891983840101No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12230.10401871146076971No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12200.10376355517754624No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11960.10172230491175846No Hit
ACTTACTACTGCCAAGAATACGATGGGTATGTGCACAGTTTTGGCCAGGGGACCAAGGTCGAGATCAAACGAA11960.10172230491175846No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGG11890.10112694025090367No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11880.10104188815649585No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCCACTTACTACTGCCAAGAATACGATGGGTATGTGCACAGTTT11860.10087178396768019No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGT11840.10070167977886456No Hit
GATCAGGAGTTTAGGGGCTTTTCCTGGCTTGTGCTGATACCAGGCCAACC11840.10070167977886456No Hit
ACCTTGGTCCCCTGGCCAAAACTGTGCACATACCCATCGTATTCTTGGCAGTAGTAAGTGGCAAAATCATCAGG11800.10036147140123326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATTA553.6379788E-11125.6278670
AACGGAA13250.074.4963170
AACGACA450.002841281565.8050770
CATCTGA25450.056.23812570
ATAAGCG200.00787045850.87800222
TGTTAGA13150.049.5414670
GTGGTAT2300.048.6783371
GTCACCG9600.047.29739470
AATGGTA4800.045.24098670
ATAATAG5700.045.02451770
TAGGCAA1551.3023782E-744.57762570
CGTATGC1400.041.7013941
CAACGGA13400.041.21592369
GACAGGT2000.040.712791
TCGTATG1350.040.6599840
CGAGACT2050.039.7164223
GTATCAA25000.039.6271171
AGACTAA2100.038.7872925
GACTAAG2050.038.08749426
ACTAAGG2100.037.19648427
Produced by FastQC (version 0.11.5)