FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1175750
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTGGCCAGGGGACCAAGGTCGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCT54430.4629385498617904No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54140.4604720391239634No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA48350.4112268764618328No Hit
ACTTTAGACAATGGGGTCCCTACAAGATTCAACGGCAGTGGCTCTGGGACAGAGTTCACTCTCACCATCAGCAGC42740.36351265149904316No Hit
GGCCAAAACTGTGCACATACCCATCGTATTCTTGGCAGTAGTAAGTGGCAAAATCATCAGGCTGCAGGCTGCT39350.33467999149479055No Hit
GTAATAGACAGCCGTGTCCGCGGCGGTCACAGAGGTCAGCCTCAGGGAGG38090.32396342759940466No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCCACTTACTACTGCCAAGAATACGATGGGTAT34010.28926217308101215No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT33330.28347863066128004No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32170.2736125877099723No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC31460.26757388900701684No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC30810.2620455028705082No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC29310.2492876887093345No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29130.24775675100999361No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28500.24239846906230064No Hit
GTCTAAAGTAGACGCCTTATGGATCAGGAGTTTAGGGGCTTTTCCTGGCT27430.23329789496066342No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC26620.22640867531362963No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA25600.21773336168403146No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25500.21688284073995323No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG23990.20403997448437167No Hit
GTCTATTACTGTGCGAGTCGCCTCTTTACAACTGATCTGCCGTATGACTACTGGGGCCAGGGAATCCTAGTCACC23930.2035296619179247No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23450.19944716138634916No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA23140.19681054645970655No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGT23030.1958749734212205No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22590.1921326812672762No Hit
GAGTGAACTCTGTCCCAGAGCCACTGCCGTTGAATCTTGTAGGGACCCCATTGTCTAAAGTAGACGCCTTATG21880.18609398256432064No Hit
GAATACGATGGGTATGTGCACAGTTTTGGCCAGGGGACCAAGGTCGAGATCAAACGAACTGTGGCTGCACCAT21730.1848182011482033No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT21460.18252179459919202No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA20530.17461194981926428No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20290.1725706995534765No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT20200.17180523070380607No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCGACCTTGGTCC19890.16916861577716352No Hit
CTACAAGATTCAACGGCAGTGGCTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTT19600.1667021050393366No Hit
GTCTCTGGTGACTCCATCACTTATCCCACTTTTTACTGGGCCTGGATCCG19410.16508611524558792No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAATCTCCTTCCACCTT19210.1633850733574314No Hit
GTATCAGCACAAGCCAGGAAAAGCCCCTAAACTCCTGATCCATAAGGCGT19040.1619391877524984No Hit
GTGCAGCCACAGTTCGTTTGATCTCGACCTTGGTCCCCTGGCCAAAACTG18730.15930257282585583No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18410.15658090580480544No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA18390.1564108016159898No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18100.1539442908781629No Hit
GTGGGGGACAGAGTCTCCATCTCTTGCCGGGCCAGTCAGAGTGTTGATATGTGGTTGGCCTGGTATCAGCACAA18010.15317882202849245No Hit
GTCCCAGAGCCACTGCCGTTGAATCTTGTAGGGACCCCATTGTCTAAAGTAGACGCCTTATGGATCAGGAGTTTA17910.1523283010844142No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17850.15181798851796727No Hit
GTGTTGATATGTGGTTGGCCTGGTATCAGCACAAGCCAGGAAAAGCCCCTAAACTCCTGATCCATAAGGCGTCTA17700.15054220710184987No Hit
ATCGTATTCTTGGCAGTAGTAAGTGGCAAAATCATCAGGCTGCAGGCTGC17390.1479055921752073No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16730.14229215394429087No Hit
ATCTTGTAGGGACCCCATTGTCTAAAGTAGACGCCTTATGGATCAGGAGTTTAGGGGCTTTTCCTGGCTTGT16710.14212204975547524No Hit
GTGCACATACCCATCGTATTCTTGGCAGTAGTAAGTGGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGT16220.13795449712949182No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16100.1369338719965979No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16090.13684881990219008No Hit
CTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCCACTTACTACTGCCAAGAATA16000.13608335105251967No Hit
GTATGTGCACAGTTTTGGCCAGGGGACCAAGGTCGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTT15840.13472251754199446No Hit
GTTGATATGTGGTTGGCCTGGTATCAGCACAAGCCAGGAAAAGCCCCTAA15730.13378694450350842No Hit
AGCCAGGAAAAGCCCCTAAACTCCTGATCCATAAGGCGTCTACTTTAGACAATGGGGTCCCTACAAGATTCAACG15630.13293642355943014No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15560.13234105889857536No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15290.1300446523495641No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGAT15260.12978949606634063No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT15160.12893897512226238No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15160.12893897512226238No Hit
CTCCTGATCCATAAGGCGTCTACTTTAGACAATGGGGTCCCTACAAGATT14980.12740803742292153No Hit
GTCCTGGGACGTGTCAACGGAAATGGCGACTCGACTCTTGAGGGACAAGTTGTAGTAGCTGATCCCGTTATAATA14320.12179459919200511No Hit
TCCATAAGGCGTCTACTTTAGACAATGGGGTCCCTACAAGATTCAACGGCAGTGGCTCTGGGACAGAGTTCACT14250.12119923453115032No Hit
GAGTGTTGATATGTGGTTGGCCTGGTATCAGCACAAGCCAGGAAAAGCCCCTAAACTCCTGATCCATAAGGCGT14160.12043376568147991No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG14150.12034871358707208No Hit
TCCCAGGACCAGTCCTCCCTGAGGCTGACCTCTGTGACCGCCGCGGACACGGCTGTCTATTACTGTGCGAGTCGC13660.11618116096108867No Hit
GTAGTAAGTGGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGAGCCACTGCCGTT13560.11533064001701043No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA13540.11516053582819477No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13440.11431001488411653No Hit
CCTTATGGATCAGGAGTTTAGGGGCTTTTCCTGGCTTGTGCTGATACCAGGCCAACCACATATCAACACTCT13310.11320433765681481No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13250.11269402509036786No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA13210.11235381671273655No Hit
GTCAGAGTGTTGATATGTGGTTGGCCTGGTATCAGCACAAGCCAGGAAAAGCCCCTAAACTCCTGATCCATAAG13210.11235381671273655No Hit
CCTTCCACCTTGTCTGCGTCTGTGGGGGACAGAGTCTCCATCTCTTGCCG13070.11116308739102701No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC13060.1110780352966192No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13030.11082287901339571No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12980.11039761854135657No Hit
GTCTCCATCTCTTGCCGGGCCAGTCAGAGTGTTGATATGTGGTTGGCCTGGTATCAGCACAAGCCAGGAAAAGCC12970.11031256644694876No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12810.10895173293642357No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAG12690.10793110780352967No Hit
CACCTGCACTGTCTCTGGTGACTCCATCACTTATCCCACTTTTTACTGGGCCTGGATCCGCCAATCCCCCGGGAA12370.10520944078247928No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT12270.10435891983840101No Hit
GTGCTGATACCAGGCCAACCACATATCAACACTCTGACTGGCCCGGCAAGAGATGGAGACTCTGTCCCCCACA12120.10308313842228366No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11950.10163725281735062No Hit
GTCTGCGTCTGTGGGGGACAGAGTCTCCATCTCTTGCCGGGCCAGTCAGA11910.10129704443971933No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC11880.10104188815649585No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGAT11870.10095683606208804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGAA14050.094.1463770
ATGGCAA1900.083.2398370
GTTATTA859.369687E-567.6601870
AATGGTA4150.058.89696570
AAACGAC200.006920257652.56081458
GTGGTAT3250.046.8117521
GGTCCAA1307.639245E-444.23934670
CTGGAGT12700.044.15226470
CCACATA17650.043.98869770
GAGCAAA24550.039.2387770
GTATCAA28350.038.280511
CGGGATA451.127813E-437.56818417
GGGATAA6900.037.5072770
ATACGTA1001.0913936E-1137.1624411
CAACGGA14100.036.49439269
GAGGGTT2051.6878248E-435.06777670
ATCAACG31550.034.4712683
TCAACGC31600.034.204414
CAACGCA31850.033.831325
GTGCTAG1201.8189894E-1233.8084871
Produced by FastQC (version 0.11.5)