FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences965658
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATAATCAGCCTCATCCTCAGACTGGAGGCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACCTGGAGCCAGA24940.2582694908549404No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24340.2520561109626804No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTC22720.23527998525357838No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG22390.2318626263128354No Hit
GATTATTCCTGTGCAGCATGGGATGACAGCCTGAATGGTCCGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTA21850.2262705844098014No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT20620.2135331556306684No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20400.21125491633683974No Hit
GAAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGCATGTTCTTGGAATTGTCTCTGGA19910.2061806560914941No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG19530.20224551549306277No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT18660.19323611464928575No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGGACCATTCAGGCTGTCATCCCAT17730.18360537581628278No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC17150.17759910858709813No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT16810.17407819331481747No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT16770.1736639679886668No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCA16020.1658972431233418No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA15940.16506879247104048No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15860.16424034181873914No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTGACTACTATGCCATGACCTGGGTCCGCCAGGCTCCAGGGAAGGG15710.16268699684567414No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA15550.16103009554107148No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC15370.1591660815733935No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG15200.15740562393725316No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14600.15119224404499315No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTA14580.15098513138191783No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC14480.14994956806654117No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG14350.1486033357565515No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14130.14632509646272282No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13820.14311485018505518No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAC13820.14311485018505518No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13780.1427006248589045No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCA13580.14062949822815118No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGGACCATTCAGG13500.13980104757584985No Hit
GTCTGAGGATGAGGCTGATTATTCCTGTGCAGCATGGGATGACAGCCTGAATGGTCCGGTATTCGGCGGAGGGAC13500.13980104757584985No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13300.1377299209450965No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG13160.13628013230356917No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGG12730.13182721004744952No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12640.13089520306361052No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAGGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGCCTCCAGT12550.12996319607977153No Hit
GTATATTTCTGTGCGAAAGGGTCTTACGATTTTTGGAGTGGTTATCCCTTTGACTACTGGGGCCAGGGAACCCGG12550.12996319607977153No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCT12410.1285134074382442No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12400.12840985110670652No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC12080.1250960484975012No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA12050.12478537950288819No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTG11970.12395692885058686No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC11910.12333559086136087No Hit
CCCCTGAGGGCCGCTGATTATTAGTATAAATGAGGAGTTTGGGGGCCGTT11890.12312847819828553No Hit
TCCTCAGACTGGAGGCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACCTGGAGCCAGAGAATCGGTCAGGGACC11660.12074668257291919No Hit
GTCCGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC11650.12064312624138153No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11470.11877911227370352No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG11430.11836488694755284No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11370.11774354895832685No Hit
GCATGGGATGACAGCCTGAATGGTCCGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAG11300.1170186546375632No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAG11260.11660442931141254No Hit
GAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTTTTCATTTGGTGATCAGGACTGAACA11240.1163973166483372No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT11150.1154653096644982No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC10950.11339418303374486No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAGCCGGCAAGTCCGTGACATGCCACGT10770.11153016906606686No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC10710.11090883107684087No Hit
GGGATAACCACTCCAAAAATCGTAAGACCCTTTCGCACAGAAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTT10600.10976971142992653No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC10590.10966615509838888No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT10580.1095625987668512No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCCGGGTTCCCTGGCCCCAGTAGTCAAAGGGATAACCA10570.10945904243531354No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGG10550.1092519297722382No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGGTCATGGCATAGTAGTCAAAGGTGAATCCAGAGGCTGCACA10520.1089412607776252No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAG10450.10821636645686153No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCT10370.1073879158045602No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACG10350.10718080314148487No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGAT10290.10655946515225886No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG10280.1064559088207212No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCCGGCCCGAGGTCCACCTGCTGCCGCC10220.10583457083149521No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10210.10573101449995753No Hit
CATTTATACTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAGGTCTGGCACCTCAGCCTC10100.1045918948530432No Hit
ATACCGGACCATTCAGGCTGTCATCCCATGCTGCACAGGAATAATCAGCCTCATCCTCAGACTGGAGGCCACT10060.10417766952689254No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT10050.10407411319535488No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA10030.10386700053227954No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAG9880.10231365555921454No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAGGCTCAGCGGGAAGACCTTGGG9800.1014852049069132No Hit
GTATGTGCTACGACCACTACCACTAATAGCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGGT9690.10034608525999887No Hit
GGCCATCAGTGGCCTCCAGTCTGAGGATGAGGCTGATTATTCCTGTGCAGCATGGGATGACAGCCTGAATGGTCC9680.10024252892846122No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)