FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences818905
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT41070.5015233757273432No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40720.497249375690709No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGG32450.3962608605393788No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT29040.3546198887538847No Hit
GTAGGAGACAGCGTCACCATCACTTGCCGGACAAGTCAGAGCATTAGCAACTATTTGAGTTGGTATCAGCAGAAA27130.33129605998253764No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26490.323480745629835No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25140.30699531691710275No Hit
GATCTATGGTGCATCCACCTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTAGATCTGGGACAGATTTCA24940.30455303118188315No Hit
GACTTACAGTCTTCCGTGCATTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCA24870.30369823117455624No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24760.3023549740201855No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC23590.28806760246915086No Hit
CTGTAAGTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGTTGCAGACCGCTGATGGTGAGAGTGAAATCTGTC23370.2853810881604093No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG22810.27854268810179444No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22770.27805423095475057No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21990.26852931658739415No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21940.26791874515358927No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21910.2675524022933063No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21110.2577832593524279No Hit
GTGCATCCACCTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTAGATCTGGGACAGATTTCACTCTCACCA20450.2497237164262033No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGCGTCACCATCACTTGCCGGACAAGTCAGAGCATTAGCAA20130.24581605924985195No Hit
CCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAACTCAAATAGTTGCTAATGCTCTGACT19920.24325165922787134No Hit
GTGGATGCACCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAACTCAAATAGTTGCTAA19390.23677960202953943No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACA19290.23555845916192966No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19240.23494788772812475No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATG19240.23494788772812475No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC18690.22823160195627085No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC18380.22444605906668053No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT18200.22224800190498287No Hit
GTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCCCCTGGCCAAAAATG18160.22175954475793896No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCCCCTGGCCAAAAATGCACGGAAGA16270.1986799445601138No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15640.1909867444941721No Hit
GATTTCACTCTCACCATCAGCGGTCTGCAACCTGAGGATTTTGCAACTTACTACTGTCAACAGACTTACAGTCT15620.19074251592065014No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15120.18463680158260115No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15010.18329354442823037No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14860.1814618301268157No Hit
GTCTGCATCTGTAGGAGACAGCGTCACCATCACTTGCCGGACAAGTCAGAGCATTAGCAACTATTTGAGTTGGTA14370.17547823007552768No Hit
GTAGTAAGTTGCAAAATCCTCAGGTTGCAGACCGCTGATGGTGAGAGTGAAATCTGTCCCAGATCTACTGCCA14270.1742570872079179No Hit
GAGTGAAATCTGTCCCAGATCTACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAGGTGGATGCACCATAGA14160.1729138300535471No Hit
ACTTACTACTGTCAACAGACTTACAGTCTTCCGTGCATTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAA14070.1718148014726983No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14030.17132634432565436No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC13770.1681513728698689No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT13690.16717445857578106No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13610.16619754428169325No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13470.16448794426703953No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13450.16424371569351756No Hit
CTGTAGGAGACAGCGTCACCATCACTTGCCGGACAAGTCAGAGCATTAGC13390.16351102997295167No Hit
AGTCAGAGCATTAGCAACTATTTGAGTTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGG13230.16155720138477603No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13020.15899280136279545No Hit
GTCTGCAACCTGAGGATTTTGCAACTTACTACTGTCAACAGACTTACAGTCTTCCGTGCATTTTTGGCCAGGGGA12780.15606205848053195No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA12760.15581782990700999No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11710.1429958297971071No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGTCACATCTGGCACCTCGGAGCCAGAG11680.14262948693682417No Hit
CACCATCAGCGGTCTGCAACCTGAGGATTTTGCAACTTACTACTGTCAACAGACTTACAGTCTTCCGTGCATTTT11600.14165257264273634No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11360.13872182976047284No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG11340.13847760118695088No Hit
GCCACAGTTCGTCTGATCTCCAGCTTGGTCCCCTGGCCAAAAATGCACGGAAGACTGTAAGTCTGTTGACAGTAG11080.1353026297311654No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10910.13322668685622877No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10900.13310457256946776No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGA10760.13139497255481405No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTG10750.13127285826805307No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT10730.1310286296945311No Hit
CTGATGGTGAGAGTGAAATCTGTCCCAGATCTACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAGGTGGA10600.12944114396663836No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10510.12834211538578957No Hit
CTATTTGAGTTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGGTGCATCCACCTTGCAAAG10490.1280978868122676No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT10130.12370177248887235No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGCGTCACCATCA10120.12357965820211135No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCG10110.12345754391535038No Hit
GGTTGCAGACCGCTGATGGTGAGAGTGAAATCTGTCCCAGATCTACTGCCACTGAACCTTGATGGGACCCCACTT10010.12223640104774057No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9980.12187005818745764No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG9890.12077102960660882No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTAGATCTGGGACAGATTTCACT9840.12016045817280392No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGTTCCACCGCAAATAGG9830.12003834388604295No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGCGTCACCATCACTTGCCGGACAAGTCAGAGCATTAGCAACTAT9820.11991622959928196No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9750.1190614295919551No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC9720.11869508673167217No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9700.11845085815815021No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9660.1179624010111063No Hit
ACCTTGATGGGACCCCACTTTGCAAGGTGGATGCACCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTC9620.11747394386406237No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9620.11747394386406237No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9610.1173518295773014No Hit
CATCAAGGTTCAGTGGCAGTAGATCTGGGACAGATTTCACTCTCACCATCAGCGGTCTGCAACCTGAGGATTT9450.11539800098912573No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGT9410.1149095438420818No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9400.11478742955532083No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9260.11307782954066711No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9240.11283360096714515No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9230.11271148668038417No Hit
GCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAACTCAAATAGTTGCTAATGCTCTGACTTGTCCGGCAAGTGA9220.1125893723936232No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGG9220.1125893723936232No Hit
ACAGTCTTCCGTGCATTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCATCTGTCTTCA9150.11173457238629633No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9110.1112461152392524No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT9080.11087977237896948No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC8970.10953651522459869No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8940.10917017236431575No Hit
GAAGACTGTAAGTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGTTGCAGACCGCTGATGGTGAGAGTGAAA8510.10391925803359364No Hit
GTCCCAGATCTACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAGGT8460.10330868659978874No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCGGTCTGCAACCTGAGGATTTTGCAACTTACTACTGTCAACAGA8390.10245388659246189No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8270.10098851515133013No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC8260.10086640086456915No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCTA358.140479E-490.15719670
TCGGACG252.8714622E-453.9603613
TCTAGCG200.007705428651.1500337
TGTTAGA8450.047.3014170
CGTATGC900.045.6697141
TCGTATG751.8189894E-1145.61595540
ACATCGA1009.780009E-442.0733670
CTTATAC1850.041.9465831
GTGGTAT1850.041.9465831
CACGAGA1850.041.9414621
CGAGACT2000.038.8124523
TCTCCGA2000.038.7840111
GAGCAAA16000.038.12898370
CCCACGA2050.037.84267419
ATCTCGT1001.0913936E-1137.5100237
CATCTCG1001.0913936E-1137.43375436
GTATCAA17350.037.3372461
TATACAC2100.036.937153
ACGAGAC2150.036.0913722
TCTTATA754.9076334E-835.9735762
Produced by FastQC (version 0.11.5)