FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004685499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences818905
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41630.5083617757859581No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA37410.45682954677282467No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG29080.35510834590092866No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT27830.33984406005580625No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27580.3367912028867817No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC25770.31468851698304445No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC23610.28831183104267283No Hit
GTAGGAGACAGCGTCACCATCACTTGCCGGACAAGTCAGAGCATTAGCAACTATTTGAGTTGGTATCAGCAGAAA23270.28415994529279953No Hit
GACTTACAGTCTTCCGTGCATTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCAT22910.27976383096940427No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22770.27805423095475057No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA21780.2659649165654136No Hit
GATCTATGGTGCATCCACCTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTAGATCTGGGACAGATTTCA21720.26523223084484765No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21360.26083611652145244No Hit
CTGTAAGTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGTTGCAGACCGCTGATGGTGAGAGTGAAATCTGTC21300.26010343080088655No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC20760.25350925931579366No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20540.2508227450070521No Hit
GTGGATGCACCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAACTCAAATAGTTGCTAAT20010.24435068780872016No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGCGTCACCATCACTTGCCGGACAAGTCAGAGCATTAGCAA19600.23934400205152004No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG19370.2365353734560175No Hit
CCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAACTCAAATAGTTGCTAATGCTCTGACTT19130.233604630573754No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGT18900.23079600197825145No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA18690.22823160195627085No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18640.22762103052246596No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18600.22713257337542203No Hit
GTGCATCCACCTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTAGA18560.22664411622837816No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18120.22127108761089503No Hit
GTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCCCCTGGCCAAAAATG17060.20832697321423121No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT16780.20490777318492379No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTA16660.203442401743792No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT16160.19733668740574303No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA15990.19526074453080638No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA15790.1928184587955868No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCAGCTTGGTCCCCTGGCCAAAAATGCACGGAA15320.18707908731782075No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14800.18072914440624982No Hit
GATTTCACTCTCACCATCAGCGGTCTGCAACCTGAGGATTTTGCAACTTACTACTGTCAACAGACTTACAGTCTT14780.18048491583272785No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14720.179752230112162No Hit
GAGTGAAATCTGTCCCAGATCTACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAGGTGGATGCACCATA14440.17633303008285456No Hit
GTAGTAAGTTGCAAAATCCTCAGGTTGCAGACCGCTGATGGTGAGAGTGAAATCTGTCCCAGATCTACTGCCA14150.17279171576678615No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCA13930.17010520145804459No Hit
GTCTGCAACCTGAGGATTTTGCAACTTACTACTGTCAACAGACTTACAGTCTTCCGTGCATTTTTGGCCAGGG13660.16680811571549814No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13560.16558697284788834No Hit
ACTTACTACTGTCAACAGACTTACAGTCTTCCGTGCATTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAA13550.16546485856112736No Hit
GTCTGCATCTGTAGGAGACAGCGTCACCATCACTTGCCGGACAAGTCAGA13390.16351102997295167No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGAT12620.15410822989235626No Hit
AGTCAGAGCATTAGCAACTATTTGAGTTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGGT12390.15129960129685371No Hit
CTGTAGGAGACAGCGTCACCATCACTTGCCGGACAAGTCAGAGCATTAGC12370.15105537272333175No Hit
CACCATCAGCGGTCTGCAACCTGAGGATTTTGCAACTTACTACTGTCAAC12350.15081114414980978No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC12320.15044480128952686No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11980.14629291553965357No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11900.1453160012455657No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT11650.14226314407654123No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11540.14091988692217045No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT11460.13994297262808264No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTAGATCTGGGACAGATTTCACTCTCACCATCAGCGGTCTGCAACCT11330.1383554869001899No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG11240.13725645831934105No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT11080.1353026297311654No Hit
GGTTGCAGACCGCTGATGGTGAGAGTGAAATCTGTCCCAGATCTACTGCC11080.1353026297311654No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10990.13420360115031657No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGT10710.13078440112100914No Hit
GCCACAGTTCGTCTGATCTCCAGCTTGGTCCCCTGGCCAAAAATGCACGG10490.1280978868122676No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10460.12773154395198466No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC10420.12724308680494076No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10400.1269988582314188No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10260.12528925821676506No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10190.12443445820943821No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA10040.12260274390802353No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9950.1215037153271747No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9930.12125948675365274No Hit
CTATTTGAGTTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGA9910.12101525818013079No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9680.11820662958462824No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCA9640.11771817243758434No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA9560.11674125814349649No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9500.11600857242293063No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCA9470.11564222956264769No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGCGTCACCATCACTTGCCG9440.11527588670236473No Hit
GTCCCAGATCTACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAGGTGGATGCACCATAGATCAGGAGCTTA9280.11332205811418908No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGAT9150.11173457238629633No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9100.11112400095249143No Hit
GCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAACTCAAATAGTTGCTAATGCTCTGACTTGTCCGGCAAGT9080.11087977237896948No Hit
CTGATGGTGAGAGTGAAATCTGTCCCAGATCTACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAGGTGGAT9070.1107576580922085No Hit
ACCTTGATGGGACCCCACTTTGCAAGGTGGATGCACCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCT8820.107704800923184No Hit
CATCAAGGTTCAGTGGCAGTAGATCTGGGACAGATTTCACTCTCACCATCAGCGGTCTGCAACCTGAGGATTTT8750.10685000091585714No Hit
GAAGACTGTAAGTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGTTGCAGACCGCTGATGGTGAGAGTGAAAT8750.10685000091585714No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGCGTCACCATCA8750.10685000091585714No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGAT8690.10611731519529129No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8680.10599520090853029No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT8650.10562885804824736No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8360.10208754373217896No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTT8340.101843315158657No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8340.101843315158657No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTG8220.10037794371752523No Hit
CCCCTAAGCTCCTGATCTATGGTGCATCCACCTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTAGATCTG8200.10013371514400328No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGG8200.10013371514400328No Hit
ACAGTCTTCCGTGCATTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGA8200.10013371514400328No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)