FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1121105
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTGTACTGATCATCTATGAACATGTCAAGCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAAATCTG34020.30345061345725866No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33530.29907992560910884No Hit
GTACAGGGGACTGGCCTGGCCTCTGCTGATACCAGCAAGCAGATTTATGCCCCAGTTTATCTCCAGAGCAGTT31640.2822215581948167No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT28200.2515375455465813No Hit
AACATATACTATGCAGACTCTGTGAAGGGCCGATTCACCGTCTCCAGAGACAACACCAAGAAGTCACTGTATCT28100.250645568434714No Hit
GTATATGTTAGTACTACTACTGGTAATACATGAAAGCCATTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAATT27830.24823723023267222No Hit
GTAATAAATAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTGACTTCTTGGTGTTGTCTCTGGA25160.22442144134581504No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG22580.20140843185963847No Hit
CCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA22190.197929721123356No Hit
ATTTATTACTGTGTGAGAGATGGGGGGGGTTGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTC22100.1971269417226754No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACAGC20870.1861556232467075No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20110.17937659719651594No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG19510.17402473452531209No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19410.17313275741344475No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19390.1729543619910713No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19140.17072441921140302No Hit
GGATTTGTGGCCTCCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCAA18410.16421298629477168No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACAGCCGTGCCGCTGTCCCACGCCTGACAATAATATTCA18210.16242903207103707No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC17900.1596639030242484No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17480.1559175991544057No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16640.1484249914147203No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16070.14334072187707664No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG15510.1383456500506197No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC15370.13709688209400547No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG15140.13504533473671065No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15030.1340641599136566No Hit
TCTATGAACATGTCAAGCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAAATCTGGGAACACAGCCACT15010.13388576449128317No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA14990.13370736906890968No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14850.13245860111229546No Hit
GGCCAGGCCAGTCCCCTGTACTGATCATCTATGAACATGTCAAGCGGCCCTCAGGGATCCCTGAGCGATTCTCTG14580.13005026291025373No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC14570.129961065199067No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCCGGCCCGAGGTCCACCTGCTGCCGCC14510.1294258789319466No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14490.12924748350957316No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14390.12835550639770582No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG14280.1273743315746518No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGATGCCCAAGTCAGGTACTTCTCGC13850.12353882999362237No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATA13630.12157648034751428No Hit
CTCCTGTGCAGCCTCTGGATTCAGCTTCAGTAATTATGAAATGAATTGGGTCCGCCAGGCTCCAGGGAAGGG12950.11551103598681658No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12790.11408387260782887No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCT12780.11399467489664217No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAACTGGTGGAGT12750.11372708176308195No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12620.11256751151765446No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAGCCGGCAAGTCCGTGACATGCCACGT12580.11221072067290752No Hit
GTACTGATCATCTATGAACATGTCAAGCGGCCCTCAGGGATCCCTGAGCG12580.11221072067290752No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG12550.11194312753934733No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12520.11167553440578715No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG12520.11167553440578715No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCGT12180.1086428122254383No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGGT11990.1069480557128904No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACAGCCAGCATCAACTGCTCTGG11850.10569928775627617No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG11830.10552089233390272No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11760.1048965083555956No Hit
GTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAACTGGTGGAGTCTGGGGGAGGCTT11670.104093728954915No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT11510.10266656557592732No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTG11430.10195298388643347No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA11360.10132859990812636No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC11340.1011502044857529No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGTA3550.070.1861170
TACCGGT150.00251075567.89831520
GATGTAT700.002685566466.7394770
TTTACCG200.00784937850.91237618
TTACCGG200.00784937850.91237619
AATCTCA950.00899445749.1764570
TGACAAT12500.047.3405370
CTGTTTA1458.826014E-442.9587470
ACTCAGT13800.041.7524770
AAGCGGT13250.039.95973270
GAGATAA17450.039.2660170
GTGGTAT2700.038.9856221
GTATCAA20450.038.6873661
GGTAGAA16500.035.86403770
GAGCCAT19650.034.86981270
CCTGCTT1800.002553768534.6056570
CCACCTT12650.034.4688770
TTCACAT2302.0754985E-433.85335570
AAGGGCA2503.3855843E-431.14508670
CGGCAAA1500.006372694431.14508470
Produced by FastQC (version 0.11.5)