FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences835982
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACATAACTACCACTGCTGTCTGCTGAT44430.5314707732941618No Hit
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC38760.4636463464524356No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG37850.45276094461363997No Hit
GTCTTTATATATCACCAACACAGGGGCCTGGCCTGGCTTCTGCTGGTACC28720.3435480668244053No Hit
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGG26760.3201025859408456No Hit
CTCTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGG25000.2990495010658124No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24610.29438432884918575No Hit
ATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGCGCGATTCTCTGGCTCCAGCTCAGGGACAACAGTCACGT24230.2898387764329854No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC22990.2750059211801211No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG21980.2629243213370623No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGCAGATGCATTGTCAAAG20420.2442636324705556No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC18600.22249282879296442No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17910.21423906256354802No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGCAGA17280.20670301513668957No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT17130.20490871813029468No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG17100.20454985872901568No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16000.19139168068211995No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15430.18457335205781944No Hit
GTGTTGGTGATATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGCGCG15320.18325753425312985No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGC15130.18098475804502967No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACATAACTACCA15090.18050627884332437No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT15030.17978856004076643No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14850.17763540363309258No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14440.17273099181561324No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG13920.16651076219344435No Hit
GTGTATTACTGTGCGAGGGGTCCCGACTTTGGAGTGGCGAAGCAACACTA13890.16615190279216538No Hit
CTATTATTGTCAATCAGCAGACAGCAGTGGTAGTTATGTGATATTCGGCGGAGGGACCAAGCTGACCGTCCTGGG13830.16543418398960744No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13700.16387912658406523No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13640.16316140778150726No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13540.16196520977724402No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13540.16196520977724402No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGCAGATGCATTGTCAAAGCAATATGCTTATTGG13490.1613671107751124No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13170.15753927716146998No Hit
CAGTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTA13140.157180417760191No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC13130.1570607979597647No Hit
TCCTCACTCTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCA13030.15586459995550142No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACATAACTACCAC13020.15574498015507512No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG12410.1484481723290693No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12160.14545767731841117No Hit
GCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTA12110.14485957831627955No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTG11790.14103174470263713No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG11470.13720391108899474No Hit
GTATTACTGTGCGAGGGGTCCCGACTTTGGAGTGGCGAAGCAACACTACTACATGGACGTCTGGGGCAAAGGGAC11430.13672543188728944No Hit
CAATAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGT11350.13576847348387883No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11220.13421341607833662No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11180.13373493687663132No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGC11020.1318210200698101No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTGTCTTTGGC10900.1303855824646942No Hit
GGCCAGGCCCCTGTGTTGGTGATATATAAAGACAGTGAGAGGCCCTCAGG10840.12966786366213628No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10800.12918938446043096No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG10770.128830525059152No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAG10440.12488307164508328No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT10260.1227299152374094No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10230.12237105583613045No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10170.12165333703357249No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10010.1197394202267513No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9980.11938056082547231No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACATAACTAC9850.11782550341993009No Hit
ACACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATATTGCTTTGACAATGCATCTGCAGAGCAGGTGA9770.11686854501651948No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC9490.11351919060458239No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9450.1130407114028771No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9440.11292109160245076No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA9330.1116052737977612No Hit
GTCCAGGCAGAAGACGAGGCTGACTATTATTGTCAATCAGCAGACAGCAG9170.10969135699094No Hit
GCTTGGTCCCTCCGCCGAATATCACATAACTACCACTGCTGTCTGCTGATTGACAATAATAGTCAGCCTCGTCT8980.10741858078283982No Hit
TCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATTATTGTCAATCAGCAGACAGCAGTGGTAGTTATGTGATAT8930.10682048178070819No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT8790.10514580457473964No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC8720.10430846597175537No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG8720.10430846597175537No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8660.10359074716919743No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC8630.10323188776791845No Hit
GGGATCCCTGAGGGCCTCTCA8630.10323188776791845No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG8620.10311226796749212No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8590.10275340856621315No Hit
GACTATTATTGTCAATCAGCAGACAGCAGTGGTAGTTATGTGATATTCGGCGGAGGGACCAAGCTGACCGTCC8520.10191606996322888No Hit
CACCAACACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATATT8510.10179645016280255No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG8500.10167683036237624No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8460.10119835116067091No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG8450.1010787313602446No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT8420.10071987195896563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1550.087.1455570
TCGTATG1100.059.24498440
CGTATGC1150.056.7136941
GTCTTTA3500.056.271391
CGCGGTA11250.054.49203570
ACGAGAC1200.053.8294922
CGAGACA1250.051.6794123
ATAGATC409.264295E-750.8987545
ATCTCCG1350.050.2703710
GTATGCC1300.050.2062642
CTTTATA3800.050.0057953
CACGAGA1300.049.68280821
TTATATA3850.049.3563655
TATATCA4000.047.5055058
TTTATAT4000.047.5055054
TCTTTAT4050.046.9190142
TATACAC1450.046.8034483
ATGCCGT1400.046.67945544
ATATATC4100.046.346837
TACACAT1400.046.051255
Produced by FastQC (version 0.11.5)