FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences835982
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCAC39930.4776418631023156No Hit
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC38910.46544064345883046No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT37440.4478565327961607No Hit
GTCTTTATATATCACCAACACAGGGGCCTGGCCTGGCTTCTGCTGGTACC28220.33756707680308906No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25950.3104133821063133No Hit
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGG25520.30526973068798136No Hit
ATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGCGCGATTCTCTGGCTCCAGCTCAGGGACAACAGTCACGTT24860.2973748238598439No Hit
CTCTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACG23730.28385778641166914No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20460.2447421116722609No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC19910.238163022648813No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGCAGATGCATTGTCAAAG19670.23529214743858123No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC17310.20706187453796854No Hit
GTGTATTACTGTGCGAGGGGTCCCGACTTTGGAGTGGCGAAGCAACACTACTACATGGACGTCTGGGGCAAAGG17270.20658339533626324No Hit
GTGTTGGTGATATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGCGCG16600.19856886870769944No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCA16340.19545875389661502No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGCAGAT15980.1911524410812673No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15900.19019548267785671No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15760.18852080547188815No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15580.18636764906421432No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG15300.1830182946522772No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATA15210.18194171644844026No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14310.17117593441007103No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGCAGATGC14070.16830505919983924No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTG13970.16710886119557597No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13380.1600512929704228No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGG13370.15993167316999649No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA13300.15909433456701222No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13090.1565823187580594No Hit
CTATTATTGTCAATCAGCAGACAGCAGTGGTAGTTATGTGATATTCGGCG12710.15203676634185903No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG12350.14773045352651132No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACATAACTACCACTGCTGTCTGCTGATTGACAATAATA12310.14725197432480602No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT12270.14677349512310076No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGG12230.14629501592139543No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT12180.1456969169192638No Hit
CAGTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGGCTGTGCTCGTGGATTCGTCCGCG12160.14545767731841117No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12120.14497919811670587No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG12110.14485957831627955No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAGCTACAGGTGTCCAGTCCCAGGTGCA12110.14485957831627955No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACATAACTACCA11680.13971592689794757No Hit
TCCTCACTCTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCA11630.13911782789581595No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCT11400.13636657248601047No Hit
GTATTACTGTGCGAGGGGTCCCGACTTTGGAGTGGCGAAGCAACACTACTACATGGACGTCTGGGGCAAAGGGAC11360.13588809328430518No Hit
GCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGGGGTCCCGACTTTGGA11270.13481151508046824No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11260.1346918952800419No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA11100.13277797847322073No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC10860.12990710326298893No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10780.12895014485957831No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10670.12763432705488875No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTGTCTTTGGC10370.124045733042099No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTT10110.12093561823101455No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGC10010.1197394202267513No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG9900.11842360242206172No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT9860.11794512322035643No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9730.11639006581481419No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACG9720.11627044601438787No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9550.11423690940714035No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9530.1139976698062877No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG9290.1111267945960559No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT9280.11100717479562956No Hit
GGCCAGGCCCCTGTGTTGGTGATATATAAAGACAGTGAGAGGCCCTCAGG9110.10897363818838203No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAA9060.10837553918625042No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACATAACTAC8990.10753820058326616No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8940.10694010158113453No Hit
TCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATTATTGTCAATCAGCA8900.10646162237942922No Hit
ACACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATATTGCTTTGACAATGCATCTGCAGAGCAGGT8710.10418884617132906No Hit
CTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTT8520.10191606996322888No Hit
GCTTGGTCCCTCCGCCGAATATCACATAACTACCACTGCTGTCTGCTGATTGACAATAATAGTCAGCCTCGTCTT8490.1015572105619499No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC8450.1010787313602446No Hit
CCATGTAGGCTGTGCTCGTGGATTCGTCCGCGGTAAGCGTGACTCTGCCCTGGAACTTCTGTGCGTAGTTT8400.10048063235811298No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG8380.10024139275726034No Hit
CAATAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGACTGTTGTCCCTGAGCTGGAGCCA8380.10024139275726034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTA11600.069.437270
AATGGTA1855.456968E-1268.745870
CAATGCA14900.067.3360170
GCTGATT18600.063.8177870
CGTTGGT1002.2788743E-456.52432370
GTCTTTA4750.054.984471
AAGCGTC200.007728751351.1110632
TTATATA5050.051.0006035
TCTTTAT5150.050.6804542
CTTTATA5150.050.007313
TATATAT5250.049.0547836
TTTATAT5350.048.1378754
ATATATC5550.046.403177
AAGCGGT6400.046.36760770
TATATCA5600.045.3837438
GGTATCA4500.045.225321
ATACTCG306.893708E-445.1820374
GAGCCAT8800.044.9625370
ACCTCTA1604.335971E-544.15962270
GACTTTA1308.3133945E-443.48024770
Produced by FastQC (version 0.11.5)