FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1366879
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAG40400.29556383556993704No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGAGTTTGGTCCCA35570.26022786215897675No Hit
CTCCTGTACAGCCTCTGGATTCATTGTCAGTAATGTCTGGATGAGTTGGG34070.24925395737296427No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC33320.243767004979958No Hit
TTACTGGACTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCAAAGACAACATCCTGAGTGGTCTTTATG31970.23389049067254675No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAG31420.22986672558434215No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG29740.21757595222400813No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC28080.20543149759415427No Hit
GGTCATTACTGGACTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCAAAGACAACATCCTGAGTGGTCT24980.18275209436972842No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC24910.18223997881304785No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24870.18194734135208748No Hit
CCCTTAGACTCTCCTGTACAGCCTCTGGATTCATTGTCAGTAATGTCTGG24450.178874648012004No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC23960.1752898391152399No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT23630.17287558006231715No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT22820.16694967147787038No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC22540.16490120925114804No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC22540.16490120925114804No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21920.1603653286062629No Hit
GATTATTACTGCCAGTCCAAAGACAACATCCTGAGTGGTCTTTATGTCTTCGGAAGTGGGACCAAACTCACCGTC21370.15634156351805828No Hit
GCCTTGGGCTGACCTAGGACGGTGAGTTTGGTCCCACTTCCGAAGACATAAAGACCACTCAGGATGTTGTCTT21360.1562684041528182No Hit
GTCTTTATGTCTTCGGAAGTGGGACCAAACTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGT21200.15509785430897685No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT21090.15429310129133597No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20950.1532688701779748No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGAGTTTGGTCCCACTTCCGAAGACATAAAGACCA20680.15129356731649252No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAG20300.14851351143736938No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG19990.14624557111492678No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG19960.14602609301920652No Hit
GGATGTTGTCTTTGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGG19640.14368499333152385No Hit
CTCCTGCACTGGGAGTAGCTCCAACATCGGGGCAAATTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGC19320.1413438936438412No Hit
ACCTAGGACGGTGAGTTTGGTCCCACTTCCGAAGACATAAAGACCACTCA18800.13753960665135687No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18780.13739328792087668No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18460.135052188233194No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG18450.13497902886795393No Hit
GACCTAGGACGGTGAGTTTGGTCCCACTTCCGAAGACATAAAGACCACTC18330.13410111648507292No Hit
GGATGAGGCTGATTATTACTGCCAGTCCAAAGACAACATCCTGAGTGGTC18220.133296363467432No Hit
GTACAGGAGAGTCTAAGGGACCCCCCAGGCTTTACCAAGGCTCCCCCAGACTCCACCAGCTGCACCTCACAC18130.13263792918027126No Hit
GTCCAAAGACAACATCCTGAGTGGTCTTTATGTCTTCGGAAGTGGGACCA18030.13190633552787043No Hit
CATAAAGACCACTCAGGATGTTGTCTTTGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGTCCAGTAA18020.13183317616263032No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17760.12993103266638817No Hit
GTCTAAGGGACCCCCCAGGCTTTACCAAGGCTCCCCCAGACTCCACCAGC17500.128028889170146No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT17310.12663886123058443No Hit
GTCCAGTAATGACCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGAT17170.12561463011722324No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA17150.12546831138674308No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG17130.12532199265626293No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTCCTTGTTGCTATTTTAAAAGG16740.12246877741189967No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16670.12195666185521908No Hit
GCCTTGGGATTCCCAAGTGTTTTCATTCAGTGATCAGGACTGAACACAGA16520.12085927137661782No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG16460.12042031518517732No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGC16350.11961556216753641No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG16140.11807921549749467No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACT15880.1161770720012525No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC15670.11464072533121072No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC15650.11449440660073057No Hit
GTGCAGGAGATGGTGATCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG15330.1121533069130479No Hit
GTAATGACCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGATTATTA15310.11200698818256774No Hit
GACAACATCCTGAGTGGTCTTTATGTCTTCGGAAGTGGGACCAAACTCACCGTCCTAGGTCAGCCCAAGGCCAAC15230.11142171326064708No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15180.11105591643444665No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG14680.10739794817244247No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT14670.10732478880720239No Hit
GGGCAGAGGATCACCATCTCCTGCACTGGGAGTAGCTCCAACATCGGGGCAAATTATGATGTACACTGGTACCAG14590.10673951388528173No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA14400.10534948594572015No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC14230.10410577673663872No Hit
GTCTGGCACCTCAGCCTCCCTGGTCATTACTGGACTCCAGGCTGAGGATG14220.10403261737139864No Hit
GTGTCTGGGGCCCCAGGGCAGAGGATCACCATCTCCTGCACTGGGAGTAGCTCCAACATCGGGGCAAATTATGA14100.10315470498851763No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC14080.10300838625803747No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT14070.1029352268927974No Hit
GGGTTCCCTGGCCCCAGGAGTCAAAGATAGGACGAACTGTTATTCCCCGATCTGTGGTACAAAAATACACGG13930.10191099577943623No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13760.1006672865703548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3700.0113.2007470
ATCGTAT252.7281736E-454.5251339
TATCGTA252.7497433E-454.43826338
ATATCGT252.7679055E-454.36575737
CGCGTAT200.007726050451.11731339
TCGTATG4000.047.79817640
CGTATGC4200.047.26207441
TATACAC4650.046.9600643
GCCGTCT4250.046.33244746
TCTCGTA3750.045.3652238
TATTCTA956.862074E-445.21918570
ATGCCGT4600.044.90138244
GGGACCG14450.043.8500970
TTATACA5050.043.2404562
CCGTCTT4650.043.16773247
CGAGACA5150.042.4070423
ACGAGAC5150.042.4039422
CACGAGA5150.042.40083721
GATCTCG3950.042.10859736
CTTATAC5200.042.005431
Produced by FastQC (version 0.11.5)