FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1366879
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55690.4074245050220246No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT35890.26256896184665945No Hit
CTCCTGTACAGCCTCTGGATTCATTGTCAGTAATGTCTGGATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGGACC34450.2520340132520874No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC33350.24398648307567822No Hit
TTACTGGACTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCAAA31880.23323205638538597No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGAGTTTGGTCCCA29900.21874650206784946No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC27490.20111509504498934No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGT26910.19687185186106454No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGG26310.1924822899466595No Hit
GGTCATTACTGGACTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT25590.1872148156493735No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC23110.16907129306983282No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAG22880.1673886276693109No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGATCACCATCTCCT22530.16482804988590796No Hit
CCCTTAGACTCTCCTGTACAGCCTCTGGATTCATTGTCAGTAATGTCTGGATGAGTTGGGTCCGCCAGGCTCCAG22270.1629259063896658No Hit
GATTATTACTGCCAGTCCAAAGACAACATCCTGAGTGGTCTTTATGTCTTCGGAAGTGGGACCAAACTCACCGTC21960.1606579660672232No Hit
GTCTTTATGTCTTCGGAAGTGGGACCAAACTCACCGTCCTAGGTCAGCCC21830.15970689431910212No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC21290.15575628859613763No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20910.15297623271701447No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC20870.1526835952560541No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT20310.14858667080260946No Hit
CTCCTGCACTGGGAGTAGCTCCAACATCGGGGCAAATTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGC19960.14602609301920652No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC19740.1444165869839247No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTA19690.14405079015772426No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19340.14149021237432136No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18760.13724696919039653No Hit
GCCTTGGGCTGACCTAGGACGGTGAGTTTGGTCCCACTTCCGAAGACATAAAGACCACTCAGGATGTTGTCTTTG18370.13439375394603326No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC18320.13402795711983284No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18270.13366216029363243No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGT18250.13351584156315227No Hit
GGATGAGGCTGATTATTACTGCCAGTCCAAAGACAACATCCTGAGTGGTC18220.133296363467432No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG18060.13212581362359066No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG17960.13139421997118986No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGAGTTTGGTCCCACTTCCGAAGACATAAAGACCA17890.13088210441450926No Hit
GTACAGGAGAGTCTAAGGGACCCCCCAGGCTTTACCAAGGCTCCCCCAGACTCCACCAGCTGCACCTCACACTG17720.12963839520542783No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17320.1267120205958245No Hit
GTCCAAAGACAACATCCTGAGTGGTCTTTATGTCTTCGGAAGTGGGACCAAACTCACCGTCCTAGGTCAGCCCAA17120.12524883329102285No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG16720.12232245868141949No Hit
GTCTAAGGGACCCCCCAGGCTTTACCAAGGCTCCCCCAGACTCCACCAGC16450.12034715581993724No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGG16440.12027399645469716No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16240.11881080914989549No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG16160.11822553422797483No Hit
GGATGTTGTCTTTGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGTCCAGTAATGACCAGGGAGGCT15880.1161770720012525No Hit
GACAACATCCTGAGTGGTCTTTATGTCTTCGGAAGTGGGACCAAACTCACCGTCCTAGGTCAGCCCAAGGCCAAC15850.11595759390553223No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTG15550.11376281294832973No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTT15520.11354333485260948No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGA15320.11208014754780782No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGATCAC15300.11193382881732766No Hit
CATAAAGACCACTCAGGATGTTGTCTTTGGACTGGCAGTAATAATCAGCC15010.10981220722536522No Hit
GTCTGGCACCTCAGCCTCCCTGGTCATTACTGGACTCCAGGCTGAGGATG14810.10834901992056356No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCT14800.10827586055532348No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCT14770.10805638245960322No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14670.10732478880720239No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC14270.10439841419759906No Hit
GCCTTGGGATTCCCAAGTGTTTTCATTCAGTGATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGA14210.10395945800615856No Hit
ACCTAGGACGGTGAGTTTGGTCCCACTTCCGAAGACATAAAGACCACTCA14190.1038131392756784No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC14000.10242311133611681No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC13930.10191099577943623No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGATCAC13900.10169151768371597No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAG13800.10095992403131515No Hit
GTCCAGTAATGACCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAAT13680.10008201164843414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGTA3600.061.7503170
TCGAGTC200.00822543450.3137635
AACAGTT10400.045.60022770
GAGCCAT18300.044.54120670
GGTATCA10000.040.2878531
ATAATAC504.6634214E-640.2510153
GTGGTAT3100.040.071251
GTATCAA24350.036.675461
AGGTACG558.956722E-636.599862
CTCGTAT2051.4127856E-436.14652670
TGGTATC3350.035.0521052
GACAGGT2750.034.183631
TTTATGT30850.033.627470
ATGGCAA1800.003256788232.933570
TATCAAC27850.032.044042
CCCGTCA22700.031.9904770
AAAAGGT17450.031.42365370
CCCTACA13750.031.25688670
TTATTAT15250.031.0978670
GTATTTT10100.030.81401670
Produced by FastQC (version 0.11.5)