FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences877760
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGTAGAGCAAGCAGTAATATTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCC36110.4113880787458986No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28700.3269686474662778No Hit
CTACTATAGTGGTGACCGAGTCTTCGGCGGAGGGACCAAGCTGACCGTCC28700.3269686474662778No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG27980.3187659496901203No Hit
CCACTATAGTAGAGCAAGCAGTAATATTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTT26850.30589227123587315No Hit
CTCCTGGACCCCTGCCCGATTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTGCGCAGC25820.2941578563616478No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACTCGGTCACCACTATAGTAGAGCAAGCAG23860.2718282901932191No Hit
GAATATTACTGCTTGCTCTACTATAGTGGTGACCGAGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG23210.2644230769230769No Hit
GTAATATTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAG22540.2567900109369304No Hit
GTCCAGGAGTGTTTGTTGTTTGTATCATAAATCAGTGTCCTGGGGGCTTG19840.22602989427633977No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA19800.225574188844331No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG19780.22534633612832666No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT19640.22375136711629603No Hit
TCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAATCGGGCAGGGGTC19140.21805504921618668No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCCCAGGCTG18990.21634615384615385No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTT18710.2131562158220926No Hit
GCCTGAGGATGAGGCTGAATATTACTGCTTGCTCTACTATAGTGGTGACCGAGTCTTCGGCGGAGGGACCAAG18160.2068902661319723No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACTCGGTCACCACTATAGTAGAGCAAGCAGTAATATTCAG18030.20540922347794385No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG17310.19720652570178637No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16910.19264947138169888No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC16760.19094057601166606No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT15900.18114290922347795No Hit
GTCCAATTCCCAGGCTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCAC15660.17840867663142546No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15630.1780668975574189No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15330.17464910681735327No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14710.16758567262121765No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG14600.1663324826831936No Hit
CTATAGTGGTGACCGAGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGG14470.16485144002916513No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT14400.16405395552314983No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14330.16325647101713453No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14310.16302861830113016No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14140.16109187021509297No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14040.1599526066350711No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14030.1598386802770689No Hit
CCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCCCAGGC13680.15585125774699235No Hit
GGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAATCGGGCAGGGGTCCAGGAGTGTTTGTTGTTTGTATCATAAA13610.15505377324097702No Hit
CCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAATCGGGCAGGGGTCCAGGAGTGTTTGTTG13330.1518638352169158No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC13070.1489017499088589No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13030.14844604447685017No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC12960.14764855997083487No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCACTTTCACTAACGCCTGG12890.14685107546481954No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGC12840.1462814436748086No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12830.14616751731680644No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCAGCACTGGAGCTGTCACCAGTGGTCA12830.14616751731680644No Hit
CAACAAACACTCCTGGACCCCTGCCCGATTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGG12750.1452561064527889No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT12540.14286365293474299No Hit
ATAGTAGAGCAAGCAGTAATATTCAGCCTCATCCTCAGGCTGCGCACCCG12160.13853445133065986No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11650.13272420707254828No Hit
ACTATAGTGGTGACCGAGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGG11640.1326102807145461No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC11520.13124316441851985No Hit
GCACTGGAGCTGTCACCAGTGGTCATTATCCCTACTGGTTCCAGCAGAAGCCTGGCCAAGCCCCCAGGACACTGA11500.13101531170251549No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC11410.12998997448049582No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTCACTAACGCCTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG11380.12964819540648923No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGA10910.12429365658038644No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10690.12178727670433831No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT10630.1211037185563252No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC10590.12064801312431643No Hit
CAGTAATATTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGC10490.11950874954429457No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAG10430.11882519139628145No Hit
CACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCAGC10410.11859733868027707No Hit
CCCTTTCGGGTGCGCAGCCTGAGGATGAGGCTGAATATTACTGCTTGCTC10350.11791378053226395No Hit
GGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAATCGGGCAGGGG10320.11757200145825737No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10290.11723022238425082No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCTGGCTGTGCCCAGCCA10250.11677451695224206No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10220.1164327378782355No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTG10160.11574917973022239No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG9910.11290102078016771No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC9620.10959715639810427No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9550.10879967189208896No Hit
GATTTATGATACAAACAACAAACACTCCTGGACCCCTGCCCGATTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGC9340.10640721837404302No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9300.10595151294203427No Hit
CTCTACTATAGTGGTGACCGAGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC9290.10583758658403207No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG9200.1048122493620124No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9110.1037869121399927No Hit
GCCTTGGGATTCCCAAGTGTTTTCATTCAGTGATCAGGACTGAACACAGA9060.10321728034998177No Hit
CTCTCACCTGTGGCTCCAGCACTGGAGCTGTCACCAGTGGTCATTATCCC9040.10298942763397739No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACTCGGTCACCA9010.10264764855997084No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC8950.10196409041195773No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC8890.10128053226394458No Hit
GTGTTTGTTGTTTGTATCATAAATCAGTGTCCTGGGGGCTTGGCCAGGCT8880.10116660590594241No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGCGACAGTCGGGGG8830.10059697411593146No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)