FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences877760
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36870.4200464819540649No Hit
CTATAGTAGAGCAAGCAGTAATATTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCC32530.3706024425811155No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28220.3215001822821728No Hit
CTCCTGGACCCCTGCCCGATTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTGCGCAGC27070.3083986511119212No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT26610.3031580386438206No Hit
CCACTATAGTAGAGCAAGCAGTAATATTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTT25210.2872083485235144No Hit
CTACTATAGTGGTGACCGAGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTC24890.2835627050674444No Hit
GAATATTACTGCTTGCTCTACTATAGTGGTGACCGAGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCA23040.2624863288370397No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACTCG22530.2566760845789282No Hit
GTAATATTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGA19820.22580204156033543No Hit
GCCTGAGGATGAGGCTGAATATTACTGCTTGCTCTACTATAGTGGTGACCGAGTCTTCGGCGGAGGGACCAA19590.2231817353262851No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCCCAGGCTGT19110.2177132701421801No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCCCAGGCTGTGGTGACTCAGGA18820.21440940576011666No Hit
GTCCAGGAGTGTTTGTTGTTTGTATCATAAATCAGTGTCCTGGGGGCTTGGCCAGGCTTCTGCTGGAACCAGTAG18410.20973842508202697No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT18250.207915603353992No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACTCGGTCACCACTATAGTAGAGCAAGCAGTAATATTCA17870.20358640174990886No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17670.20130787458986513No Hit
TCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAATCGGGCAGGGGTC17590.2003964637258476No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG17470.19902934742982137No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA17460.1989154210718192No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA17080.19458621946773608No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTA16980.19344695588771418No Hit
GTCCAATTCCCAGGCTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCAC16540.18843419613561793No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15140.1724845060153117No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC14800.16861100984323735No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA14780.16838315712723295No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14400.16405395552314983No Hit
CTATAGTGGTGACCGAGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT13630.15528162595698142No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAG13190.15026886620488517No Hit
CCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCCCAGGCTGTGGTGACTCAGGAGCCCTCACT13120.14947138169886984No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAA12990.1479903390448414No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG12580.14331935836675172No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12500.14240794750273422No Hit
CCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAATCG12360.1408129784907036No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC12330.14047119941669706No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT12240.13944586219467736No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCA12220.139218009478673No Hit
GGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAATCGGGCAGGGGTCCAG12200.13899015676266863No Hit
CAACAAACACTCCTGGACCCCTGCCCGATTCTCAGGCTCCCTCCTTGGGG11830.13477488151658767No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11790.1343191760845789No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCACTTTCACTAACGCCTGGATGAGCTGGGTCCGCCAGGCTCCAG11760.13397739701057237No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGG11740.13374954429456798No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTG11650.13272420707254828No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCT11570.1318127962085308No Hit
ATAGTAGAGCAAGCAGTAATATTCAGCCTCATCCTCAGGCTGCGCACCCG11430.13021782719650019No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAG11300.12873678454247173No Hit
CACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCAGC11090.12634433102442583No Hit
ACTATAGTGGTGACCGAGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTA10960.12486328837039737No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTCACTAACGCCTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGG10920.12440758293838863No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGAT10820.12326831935836675No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGG10790.12292654028436018No Hit
GCACTGGAGCTGTCACCAGTGGTCATTATCCCTACTGGTTCCAGCAGAAGCCTGGCCAAGCCCCCAGGACACT10730.12224298213634706No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCAGCACTGGA10550.1201923076923077No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10550.1201923076923077No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACG10360.11802770689026613No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC10160.11574917973022239No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAG10070.1147238425082027No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT10050.11449598979219831No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGTCTGGGCACTGTGTGGCC10030.11426813707619396No Hit
AAGCTGCCCTGACCCTTTCGGGTGCGCAGCCTGAGGATGAGGCTGAATATTACTGCTTGCTCTACTATAGTGGT9910.11290102078016771No Hit
CAGTAATATTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGC9860.11233138899015677No Hit
CCCTTTCGGGTGCGCAGCCTGAGGATGAGGCTGAATATTACTGCTTGCTCTACTATAGTGGTGACCGAGTCTTCG9700.11050856726212176No Hit
GATTTATGATACAAACAACAAACACTCCTGGACCCCTGCCCGATTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGC9690.11039464090411959No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC9640.10982500911410865No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTTCAGGGCCAGCTCCTCCGACGGCGGC9610.10948323004010208No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9430.1074325555960627No Hit
CTCTCACCTGTGGCTCCAGCACTGGAGCTGTCACCAGTGGTCATTATCCC9170.10447047028800582No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAACGTGGTCGTCGCATGCCTGGTCCAGG9150.10424261757200147No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9090.10355905942398833No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA9070.10333120670798394No Hit
ATATTACTGCTTGCTCTACTATAGTGGTGACCGAGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC9050.10310335399197959No Hit
GGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAATCGGGCAGGGG9030.10287550127597521No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9020.10276157491797301No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTG8960.1020780167699599No Hit
CTCTACTATAGTGGTGACCGAGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC8950.10196409041195773No Hit
GTGTTTGTTGTTTGTATCATAAATCAGTGTCCTGGGGGCTTGGCCAGGCTTCTGCTGGAACCAGTAGGGATAAT8850.10082482683193583No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACTCGGTCACCA8800.10025519504192489No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC8790.10014126868392272No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTCGT255.769654E-5175.2836970
CAAAGTA750.004547441458.427970
GGTAGAA19450.051.0680970
TATGGTT900.00935264548.68991570
GATGAGT900.00935264548.68991570
AAATCCA3850.045.52823370
CAGCCAT9650.045.41028670
GACAGGT1550.043.846131
GGTATCA7400.042.246341
GTGGTAT2150.041.0930021
GTATCAA18800.039.0417181
ATCTGGT1909.825531E-538.43940470
TGGTATC2350.037.555032
AATGGTA1700.002640013634.36935470
TATCAAC21150.034.0242162
AAAGGCA7300.034.01624370
ACGGGTA1800.033.9633216
ATCAACG21950.033.2442933
GCACCGT8000.032.86569270
CATAAAT13350.032.8246670
Produced by FastQC (version 0.11.5)