FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004685510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences756596
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33210.43893967189887334No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT30630.4048395709202798No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24130.318928463803668No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20960.2770302777175666No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT20740.2741225171690043No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20120.26592791925941983No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20050.2650027227212409No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19530.258129834151912No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGG19340.2556185864054264No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18500.24451622794727967No Hit
GTAATAAACAGCCGTGTCCCCGGCTCTCAGGCTGTCCGTTTGAAGAAACA18450.24385537327715187No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17530.23169564734680068No Hit
GTTTATTACTGTGCCAGAGATGGGGGCATTTCTATGGTTCGGGGAGTTAT17100.22601229718370175No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAAAACATTGCCACTTATTTAAACTGGTATCAACAGAAA17050.22535144251357395No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC15960.2109448107047883No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT15400.20354323839935712No Hit
GATTTCACTCTCACCATCAACAGCCTGCAACCTGAAGATTTTGCAACTTT14940.19746337543418152No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14940.19746337543418152No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG14180.18741838444823922No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13800.18239588895526807No Hit
GTCTCCATCCTCCCTGTCTGCCTCGGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAAAACATTGCCA13610.1798846412087825No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC13260.17525865851788802No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12960.17129353049712132No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12590.16640320593817573No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12480.16494932566389459No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12300.1625702488514346No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGATATCCAGATGACCCAGTC12170.16085202670910237No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGATA11950.1579442661605401No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11890.15715124055638677No Hit
GCGTAGATCAGGAGCTGAGGGGCTTTACCTGGTTTCTGTTGATACCAGTTTAAATAAGTGGCAATGTTTTGACT11650.1539791381397734No Hit
CACCATCAACAGCCTGCAACCTGAAGATTTTGCAACTTTCTACTGTCAACAGAATTTCTATCTGATTACTTTCGG11410.15080703572316004No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATG11300.14935315544887892No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11120.1469740786364189No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT11080.14644539490031666No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGAGCTCCACCTTGGTCC11060.14618105303226556No Hit
GTTGATGGTGAGAGTGAAATCCGTCCCAGATCCACTGCCACTGAACCTCGATGGGGCCCCAGTTTGTAAATTGGA11050.14604888209824002No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10840.14327329248370332No Hit
AGTCAAAACATTGCCACTTATTTAAACTGGTATCAACAGAAACCAGGTAAAGCCCCTCAGCTCCTGATCTACG10830.14314112154967776No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG10510.13891165166085995No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10370.13706125858450216No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10240.13534303644216994No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10230.13521086550814437No Hit
GATCTACGCTGCATCCAATTTACAAACTGGGGCCCCATCGAGGTTCAGTGGCAGTGGATCTGGGACGGATTTCA10190.13468218177204216No Hit
GAGTGAAATCCGTCCCAGATCCACTGCCACTGAACCTCGATGGGGCCCCAGTTTGTAAATTGGATGCAGCGTAGA10130.13388915616788882No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA10100.13349264336581212No Hit
GGACCAAGGTGGAGCTCAAGCGAACTGTGGCTGCACCATCTGTCTTCATC9980.13190659215750547No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9890.13071705375127543No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9870.13045271188322433No Hit
TATTTAAACTGGTATCAACAGAAACCAGGTAAAGCCCCTCAGCTCCTGATCTACGCTGCATCCAATTTACAAA9800.12952751534504545No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9800.12952751534504545No Hit
CTCCTGTGCAGCGTCTGGATTCACCTTCAGTGATTATAGGATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG9670.1278092932027132No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9450.12490153265415095No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG9300.12291896864376761No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT9250.12225811397363984No Hit
GTGATATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCCTCGGTAGGAGACAGAGTCACCATCACTTGCCGGG9240.12212594303961427No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG9230.12199377210558872No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA9130.12067206276533315No Hit
CCATCAACAGCCTGCAACCTGAAGATTTTGCAACTTTCTACTGTCAACAG9130.12067206276533315No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9130.12067206276533315No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9100.12027554996325647No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9080.12001120809520535No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8800.11631042194248978No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC8710.11512088353625978No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8530.11274180672379976No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8530.11274180672379976No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8490.11221312298769753No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC8490.11221312298769753No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGATATCCAGATGACCCAGTCTCCATCCTCC8450.1116844392515953No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8400.11102358458146752No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8400.11102358458146752No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8360.1104949008453653No Hit
GATCAGGAGCTGAGGGGCTTTACCTGGTTTCTGTTGATACCAGTTTAAAT8340.11023055897731418No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8310.10983404617523752No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8260.10917319150510972No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8200.10838016590095638No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8200.10838016590095638No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT8080.1067941146926497No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT7950.10507589255031746No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT7840.10362201227603635No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT7790.10296115760590858No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCG7720.10203596106772966No Hit
CCATCCTCCCTGTCTGCCTCGGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAAAACATTGCCACTTAT7690.101639448265653No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7680.10150727733162744No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)