FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences756596
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA31160.41184463042363434No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30140.39836319515302754No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23870.3154920195190035No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC20760.27438685903705545No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT19450.2570724666797075No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19330.25548641547140083No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA17980.2376433393779507No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG17660.2334138694891329No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17380.22971308333641732No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA17210.22746617745798287No Hit
GTAATAAACAGCCGTGTCCCCGGCTCTCAGGCTGTCCGTTTGAAGAAACACTGTGTTGTTGGAGTTGTCTCTGGA16450.21742118647204056No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16380.21649598993386168No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA15590.20605448614584268No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15040.1987850847744371No Hit
GTTTATTACTGTGCCAGAGATGGGGGCATTTCTATGGTTCGGGGAGTTATAACAACTTATGGTTTGGACGTCTGG15010.1983885719723604No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC14820.19587732422587484No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14610.19310173461133817No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAAAACATTGCCACTTATTTAAACTGGTATCAACAGAAA14210.1878148972503159No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC14190.18755055538226476No Hit
GATTTCACTCTCACCATCAACAGCCTGCAACCTGAAGATTTTGCAACTTTCTACTGTCAACAGAATTTCTATCT12880.17023616302491687No Hit
GTCTCCATCCTCCCTGTCTGCCTCGGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAAAACATTGCCA12750.16851794088258462No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12730.16825359901453352No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA12340.16309893258753683No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT11920.15754775335846344No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG11800.15596170215015676No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGT11780.15569736028210565No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11740.15516867654600341No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11390.15054269385510893No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGAGCTCCACCTTGGTCC11350.1500140101190067No Hit
CACCATCAACAGCCTGCAACCTGAAGATTTTGCAACTTTCTACTGTCAACAGAATTTCTATCTGATTACTTTCG11190.1478992751745978No Hit
GATCTACGCTGCATCCAATTTACAAACTGGGGCCCCATCGAGGTTCAGTGGCAGTGGATCTGGGACGGATTTCA11180.14776710424057224No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGATATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCCTCGGTA11150.1473705914384956No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10910.1441984890218822No Hit
GCGTAGATCAGGAGCTGAGGGGCTTTACCTGGTTTCTGTTGATACCAGTTTAAATAAGTGGCAATGTTTTGACTT10720.14168724127539664No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGATAT10660.1408942156712433No Hit
GTTGATGGTGAGAGTGAAATCCGTCCCAGATCCACTGCCACTGAACCTCGATGGGGCCCCAGTTTGTAAATTG10520.1390438225948855No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10370.13706125858450216No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10250.1354752073761955No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10140.13402132710191436No Hit
GAGTGAAATCCGTCCCAGATCCACTGCCACTGAACCTCGATGGGGCCCCAGTTTGTAAATTGGATGCAGCGTA9920.13111356655335213No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9870.13045271188322433No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9710.12833797693881543No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG9710.12833797693881543No Hit
AGTCAAAACATTGCCACTTATTTAAACTGGTATCAACAGAAACCAGGTAAAGCCCCTCAGCTCCTGATCTAC9440.12476936172012541No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9380.12397633611597207No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC9370.1238441651819465No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9300.12291896864376761No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9260.12239028490766539No Hit
CTCCTGTGCAGCGTCTGGATTCACCTTCAGTGATTATAGGATGCACTGGGTCCGCCAGGCTCCAGGCAAGGG9190.1214650883694865No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9070.11987903716117981No Hit
GGACCAAGGTGGAGCTCAAGCGAACTGTGGCTGCACCATCTGTCTTCATC9010.11908601155702647No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8790.11617825100846424No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT8770.11591390914041312No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA8680.1147243707341831No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8680.1147243707341831No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8650.11432785793210644No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8630.11406351606405532No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8400.11102358458146752No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA8310.10983404617523752No Hit
CCATCAACAGCCTGCAACCTGAAGATTTTGCAACTTTCTACTGTCAACAGAATTTCTATCTGATTACTTTCGGCG8280.10943753337316084No Hit
TATTTAAACTGGTATCAACAGAAACCAGGTAAAGCCCCTCAGCTCCTGATCTACGCTGCATCCAATTTACAAA8240.10890884963705862No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8100.10705845656070082No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC8090.10692628562667526No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGATATCCAGAT8060.1065297728245986No Hit
CCTTGGTCCCGCCGCCGAAAGTAATCAGATAGAAATTCTGTTGACAGTAGAAAGTTGCAAAATCTTCAGGTTGCA8050.10639760189057304No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCA7940.10494372161629192No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC7850.1037541832100619No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG7820.10335767040798524No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT7760.1025646448038319No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7710.10190379013370413No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7690.101639448265653No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA150.0029476334194.4118270
TTACGCT150.002219551270.0399761
GTAGTTT901.1587956E-464.8039470
CCGCTAT700.00348399462.4895170
AACCTTT1701.2412784E-860.0389470
AATGGTA2004.4732587E-851.033170
ACGGTAC200.00785705250.8981828
CGAGAGT200.00785910450.894824
AAGTTGT4600.050.71612570
CGATTCA4700.049.6370670
TAGGCAA2555.846232E-945.74395870
CCTACTT1307.1222027E-444.8642770
AAAGTCA2452.2071981E-741.65967670
GCCATTT1500.00144015138.88236270
GAGCCAT7900.038.75931570
AAACTCA1550.001691897337.62809470
GAGCAAA18650.037.52721470
GGAGGAA3305.6837962E-835.34760770
ATCAACG21150.034.972643
AGCGGGT400.002467483734.858358
Produced by FastQC (version 0.11.5)