FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences884135
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGTTATAGTGCTGGCAGTAATAAGTTGCAAGATCATCAGGCTGCAGGCTGGCGATGGTGAGAGTGAATTCTGTC41400.46825428243424366No Hit
CTATAACGGTTACCCCATCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCA41080.4646349256618051No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT34680.39224779021303313No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34010.38466976197073977No Hit
CTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTG25740.29113201038302977No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT23220.2626295758000758No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22960.2596888484224694No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22010.24894388300429232No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC21570.24396726744218925No Hit
GTCTTAGAAACTGAGGTCCCGTCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCGCCAGC21100.23865133718267006No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20750.23469266571281536No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19700.2228166513032512No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19680.22259044150497379No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC19580.2214593925135867No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18500.20924406340660645No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18470.20890474870919035No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17880.2022315596600067No Hit
GAATTCACTCTCACCATCGCCAGCCTGCAGCCTGATGATCTTGCAACTTATTACTGCCAGCACTATAACGGTTAC17810.20143982536603572No Hit
CCTTCCACCCTGTCTGCATCTATTGGAGACAGAGTCATCATCACTTGCCGGGCCAGTCAGAGTCTTAGTGGCTGG17620.1992908322824003No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG16840.19046865014958123No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16670.18854586686422323No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT16640.1882065521668071No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC15020.1698835585063367No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGGGCCAATAGTGACA15010.169770453607198No Hit
GTCAGAGTCTTAGTGGCTGGTTGGCCTGGTATCAGCAGAAACCCGGGAAAGCCCCTAAGCTCCTAATCTATAAGG14940.16897871931322705No Hit
TCTATAAGGCGTCTGTCTTAGAAACTGAGGTCCCGTCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCAC14890.16841319481753353No Hit
GTCTGCATCTATTGGAGACAGAGTCATCATCACTTGCCGGGCCAGTCAGAGTCTTAGTGGCTGGTTGGCCTGGTA14410.1629841596588756No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGGGCCAATAG14190.1604958518778241No Hit
GGCCAATAGTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTATTGGAGACAGAGTCATCATCA13980.15812064899591124No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13730.15529302651744362No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13620.15404887262691783No Hit
GTAATACACGGCCGTGTCCTCGGCTTTAAGGCTGTTCATTTGCAGATACA13520.15291782363553078No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13420.1517867746441437No Hit
GAGTCTTAGTGGCTGGTTGGCCTGGTATCAGCAGAAACCCGGGAAAGCCCCTAAGCTCCTAATCTATAAGGCG13270.15009020115706312No Hit
ATTGGAGACAGAGTCATCATCACTTGCCGGGCCAGTCAGAGTCTTAGTGGCTGGTTGGCCTGGTATCAGCAGAAA13110.14828052277084383No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCC12960.14658394928376323No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGGGCCAA12770.1444349562001278No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12620.1427383827130472No Hit
GTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG12350.13968455043630215No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12210.13810108184836026No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12180.13776176715094415No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11890.13448172507592165No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11820.1336899907819507No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11780.1332375711853959No Hit
CCTTATAGATTAGGAGCTTAGGGGCTTTCCCGGGTTTCTGCTGATACCAGGCCAACCAGCCACTAAGACTCTGA11750.13289825648797976No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGACGGGACCTCAGTTTCTAAGACAGACGCCTTATAGA11660.13188031239573142No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11640.131654102597454No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGGGCCAATAGTGACATCCAGATGACCCAGTCTCCTTCCACC11530.13040994870692824No Hit
GTCTCCTTCCACCCTGTCTGCATCTATTGGAGACAGAGTCATCATCACTTGCCGGGCCAGTCAGAGTCTTAG11340.12826095562329282No Hit
GTCTTAGTGGCTGGTTGGCCTGGTATCAGCAGAAACCCGGGAAAGCCCCTAAGCTCCTAATCTATAAGGCG11290.1276954311275993No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA11070.12520712334654777No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10830.1224926057672188No Hit
GGTATCAGCAGAAACCCGGGAAAGCCCCTAAGCTCCTAATCTATAAGGCGTCTGTCTTAGAAACTGAGGTCCCG10730.12136155677583176No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10730.12136155677583176No Hit
GATTAGGAGCTTAGGGGCTTTCCCGGGTTTCTGCTGATACCAGGCCAACC10620.12011740288530598No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTG10560.11943877349047374No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10450.11819461959994798No Hit
ACTTATTACTGCCAGCACTATAACGGTTACCCCATCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAA10410.11774220000339314No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10330.1168373608102835No Hit
GTCCATGTAGTGGTCACTGAAGCTGAATCCAGACGCTACACAGGAGAGTC10230.11570631181889643No Hit
CTCCTGTGTAGCGTCTGGATTCAGCTTCAGTGACCACTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGG10190.11525389222234161No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT10100.11423594813009326No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT9750.11027727666023854No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9740.11016417176109984No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC9560.10812828357660313No Hit
GTGATGGGGTAACCGTTATAGTGCTGGCAGTAATAAGTTGCAAGATCATC9540.1079020737783257No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9470.10711033948435478No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC9440.10677102478693865No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG9430.10665791988779993No Hit
GTAATAAGTTGCAAGATCATCAGGCTGCAGGCTGGCGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG9380.10609239539210642No Hit
CCTCAGAATACGCCGCGTCTGTGAAAGGCAGATTTACCGTCTCAAGAGAT9370.1059792904929677No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9300.10518755619899677No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9280.10496134640071933No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9180.10383029740933228No Hit
GGGTTGTAGTAACGAGTAGTTGCTGTCCCCCCCCTGGTACAGTAATACACGGCCGTGTCCTCGGCTTTAAGG9080.10269924841794521No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9010.10190751412397427No Hit
CGGTTACCCCATCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCATCTGTCTTCA8950.10122888472914204No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8900.1006633602334485No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)